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Microflow-Based Device for In Vitro and Ex Vivo Drug Permeability Studies

Research output: Contribution to journalArticleScientificpeer-review

Details

Original languageEnglish
JournalSLAS Technology
DOIs
Publication statusE-pub ahead of print - 2020
Publication typeA1 Journal article-refereed

Abstract

This paper presents a novel microflow-based concept for studying the permeability of in vitro cell models or ex vivo tissues. Using the proposed concept, we demonstrate how to maintain physiologically relevant test conditions and produce highly reproducible permeability values for a range (31) of drug compounds. The apparent permeability coefficients (Papp) showed excellent correlation (0.89) with the values from experiments performed with a conventional Ussing chamber. Additionally, the microflow-based concept produces notably more concentrated samples than the conventional Ussing chamber-based approach, despite the fact that more than 10 times smaller quantities of test compounds and biological membranes are needed in the microflow-based concept.

Keywords

  • ex vivo, in vitro, microfluidics, permeability

Publication forum classification

Field of science, Statistics Finland