Tampere University of Technology

TUTCRIS Research Portal

MIR-25 modulates invasivenessand dissemination of human prostate cancer cells via regulation of αv-and α6-integrin expression

Research output: Contribution to journalArticleScientificpeer-review

Standard

MIR-25 modulates invasivenessand dissemination of human prostate cancer cells via regulation of αv-and α6-integrin expression. / Zoni, E.; Van Der Horst, G.; Van De Merbel, A. F.; Chen, L.; Rane, J. K.; Pelger, R. C M; Collins, A. T.; Visakorpi, T.; Snaar-Jagalska, B. E.; Maitland, N. J.; Van Der Pluijm, G.

In: Cancer Research, Vol. 75, No. 11, 01.06.2015, p. 2326-2336.

Research output: Contribution to journalArticleScientificpeer-review

Harvard

Zoni, E, Van Der Horst, G, Van De Merbel, AF, Chen, L, Rane, JK, Pelger, RCM, Collins, AT, Visakorpi, T, Snaar-Jagalska, BE, Maitland, NJ & Van Der Pluijm, G 2015, 'MIR-25 modulates invasivenessand dissemination of human prostate cancer cells via regulation of αv-and α6-integrin expression', Cancer Research, vol. 75, no. 11, pp. 2326-2336. https://doi.org/10.1158/0008-5472.CAN-14-2155

APA

Zoni, E., Van Der Horst, G., Van De Merbel, A. F., Chen, L., Rane, J. K., Pelger, R. C. M., ... Van Der Pluijm, G. (2015). MIR-25 modulates invasivenessand dissemination of human prostate cancer cells via regulation of αv-and α6-integrin expression. Cancer Research, 75(11), 2326-2336. https://doi.org/10.1158/0008-5472.CAN-14-2155

Vancouver

Zoni E, Van Der Horst G, Van De Merbel AF, Chen L, Rane JK, Pelger RCM et al. MIR-25 modulates invasivenessand dissemination of human prostate cancer cells via regulation of αv-and α6-integrin expression. Cancer Research. 2015 Jun 1;75(11):2326-2336. https://doi.org/10.1158/0008-5472.CAN-14-2155

Author

Zoni, E. ; Van Der Horst, G. ; Van De Merbel, A. F. ; Chen, L. ; Rane, J. K. ; Pelger, R. C M ; Collins, A. T. ; Visakorpi, T. ; Snaar-Jagalska, B. E. ; Maitland, N. J. ; Van Der Pluijm, G. / MIR-25 modulates invasivenessand dissemination of human prostate cancer cells via regulation of αv-and α6-integrin expression. In: Cancer Research. 2015 ; Vol. 75, No. 11. pp. 2326-2336.

Bibtex - Download

@article{b2d43edcea5f4dd7a4f79538d013ed97,
title = "MIR-25 modulates invasivenessand dissemination of human prostate cancer cells via regulation of αv-and α6-integrin expression",
abstract = "Altered microRNA (miRNA; miR) expression is associated with tumor formation and progression of various solid cancers. A major challenge in miRNA expression profiling of bulk tumors is represented by the heterogeneity of the subpopulations of cells that constitute the organ, as well as the tumor tissue. Here, we analyzed the expression of miRNAs in a subpopulation of epithelial stem/progenitor-like cells in human prostate cancer [prostate cancer stem cell (PCSC)] and compared their expression profile to more differentiated cancer cells. In both cell lines and clinical prostate cancer specimens, we identified that miR-25 expression in PCSCs was low/absent and steadily increased during their differentiation into cells with a luminal epithelial phenotype. Functional studies revealed that overexpression of miR-25 in prostate cancer cell lines and selected subpopulation of highly metastatic and tumorigenic cells (ALDHhigh) strongly affected the invasive cytoskeleton, causing reduced migration in vitro and metastasis via attenuation of extravasation in vivo. Here, we show, for the first time, that miR-25 can act as a tumor suppressor in highly metastatic PCSCs by direct functional interaction with the 3′-untranslated regions of proinvasive avand a6-integrins. Taken together, our observations suggest that miR-25 is a key regulator of invasiveness in human prostate cancer through its direct interactions with αv-and α6-integrin expression. Cancer Res; 75(11); 2326-36.",
author = "E. Zoni and {Van Der Horst}, G. and {Van De Merbel}, {A. F.} and L. Chen and Rane, {J. K.} and Pelger, {R. C M} and Collins, {A. T.} and T. Visakorpi and Snaar-Jagalska, {B. E.} and Maitland, {N. J.} and {Van Der Pluijm}, G.",
year = "2015",
month = "6",
day = "1",
doi = "10.1158/0008-5472.CAN-14-2155",
language = "English",
volume = "75",
pages = "2326--2336",
journal = "Cancer Research",
issn = "0008-5472",
publisher = "American Association for Cancer Research",
number = "11",

}

RIS (suitable for import to EndNote) - Download

TY - JOUR

T1 - MIR-25 modulates invasivenessand dissemination of human prostate cancer cells via regulation of αv-and α6-integrin expression

AU - Zoni, E.

AU - Van Der Horst, G.

AU - Van De Merbel, A. F.

AU - Chen, L.

AU - Rane, J. K.

AU - Pelger, R. C M

AU - Collins, A. T.

AU - Visakorpi, T.

AU - Snaar-Jagalska, B. E.

AU - Maitland, N. J.

AU - Van Der Pluijm, G.

PY - 2015/6/1

Y1 - 2015/6/1

N2 - Altered microRNA (miRNA; miR) expression is associated with tumor formation and progression of various solid cancers. A major challenge in miRNA expression profiling of bulk tumors is represented by the heterogeneity of the subpopulations of cells that constitute the organ, as well as the tumor tissue. Here, we analyzed the expression of miRNAs in a subpopulation of epithelial stem/progenitor-like cells in human prostate cancer [prostate cancer stem cell (PCSC)] and compared their expression profile to more differentiated cancer cells. In both cell lines and clinical prostate cancer specimens, we identified that miR-25 expression in PCSCs was low/absent and steadily increased during their differentiation into cells with a luminal epithelial phenotype. Functional studies revealed that overexpression of miR-25 in prostate cancer cell lines and selected subpopulation of highly metastatic and tumorigenic cells (ALDHhigh) strongly affected the invasive cytoskeleton, causing reduced migration in vitro and metastasis via attenuation of extravasation in vivo. Here, we show, for the first time, that miR-25 can act as a tumor suppressor in highly metastatic PCSCs by direct functional interaction with the 3′-untranslated regions of proinvasive avand a6-integrins. Taken together, our observations suggest that miR-25 is a key regulator of invasiveness in human prostate cancer through its direct interactions with αv-and α6-integrin expression. Cancer Res; 75(11); 2326-36.

AB - Altered microRNA (miRNA; miR) expression is associated with tumor formation and progression of various solid cancers. A major challenge in miRNA expression profiling of bulk tumors is represented by the heterogeneity of the subpopulations of cells that constitute the organ, as well as the tumor tissue. Here, we analyzed the expression of miRNAs in a subpopulation of epithelial stem/progenitor-like cells in human prostate cancer [prostate cancer stem cell (PCSC)] and compared their expression profile to more differentiated cancer cells. In both cell lines and clinical prostate cancer specimens, we identified that miR-25 expression in PCSCs was low/absent and steadily increased during their differentiation into cells with a luminal epithelial phenotype. Functional studies revealed that overexpression of miR-25 in prostate cancer cell lines and selected subpopulation of highly metastatic and tumorigenic cells (ALDHhigh) strongly affected the invasive cytoskeleton, causing reduced migration in vitro and metastasis via attenuation of extravasation in vivo. Here, we show, for the first time, that miR-25 can act as a tumor suppressor in highly metastatic PCSCs by direct functional interaction with the 3′-untranslated regions of proinvasive avand a6-integrins. Taken together, our observations suggest that miR-25 is a key regulator of invasiveness in human prostate cancer through its direct interactions with αv-and α6-integrin expression. Cancer Res; 75(11); 2326-36.

UR - http://www.scopus.com/inward/record.url?scp=84936888406&partnerID=8YFLogxK

U2 - 10.1158/0008-5472.CAN-14-2155

DO - 10.1158/0008-5472.CAN-14-2155

M3 - Article

VL - 75

SP - 2326

EP - 2336

JO - Cancer Research

JF - Cancer Research

SN - 0008-5472

IS - 11

ER -