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Molecular tools for selective recovery and detection of lignin-derived molecules

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Molecular tools for selective recovery and detection of lignin-derived molecules. / Salmela, Milla; Sanmark, Hanna; Efimova, Elena; Efimov, Alexander; Hytönen, Vesa P.; Lamminmäki, Urpo; Santala, Suvi; Santala, Ville.

In: Green Chemistry, Vol. 20, No. 12, 21.06.2018, p. 2829-2839.

Research output: Contribution to journalArticleScientificpeer-review

Harvard

Salmela, M, Sanmark, H, Efimova, E, Efimov, A, Hytönen, VP, Lamminmäki, U, Santala, S & Santala, V 2018, 'Molecular tools for selective recovery and detection of lignin-derived molecules', Green Chemistry, vol. 20, no. 12, pp. 2829-2839. https://doi.org/10.1039/c8gc00490k

APA

Salmela, M., Sanmark, H., Efimova, E., Efimov, A., Hytönen, V. P., Lamminmäki, U., ... Santala, V. (2018). Molecular tools for selective recovery and detection of lignin-derived molecules. Green Chemistry, 20(12), 2829-2839. https://doi.org/10.1039/c8gc00490k

Vancouver

Salmela M, Sanmark H, Efimova E, Efimov A, Hytönen VP, Lamminmäki U et al. Molecular tools for selective recovery and detection of lignin-derived molecules. Green Chemistry. 2018 Jun 21;20(12):2829-2839. https://doi.org/10.1039/c8gc00490k

Author

Salmela, Milla ; Sanmark, Hanna ; Efimova, Elena ; Efimov, Alexander ; Hytönen, Vesa P. ; Lamminmäki, Urpo ; Santala, Suvi ; Santala, Ville. / Molecular tools for selective recovery and detection of lignin-derived molecules. In: Green Chemistry. 2018 ; Vol. 20, No. 12. pp. 2829-2839.

Bibtex - Download

@article{8ce1259f886c473c89d301a3655e7cbc,
title = "Molecular tools for selective recovery and detection of lignin-derived molecules",
abstract = "The pulp and paper industry together with lignocellulosic biofuel production provides plentiful streams of lignin and lignin-derived molecules (LDMs) that currently remain underutilized. The heterogeneity and complexity of lignin along with the lack of convenient tools significantly hamper its utilization. Selective separation of these LDMs from streams using specific tools would allow the recovery of aromatic compounds, as well as facilitate biological processes aiming at lignin valorization. To this end, here we report the isolation and characterization of single-chain variable fragment (scFv) antibodies against ferulate, coumarate, and caffeate, which are the molecular representatives of LDMs. Binders for the target LDMs were enriched by interrogating a synthetic scFv library with the phage display technique. As a result, scFv binders specific against each of the target molecules were obtained with affinities in the micromolar range. The selectivity of scFvs towards specific LDMs was proved by recovering caffeate from simulated LDM solution, Kraft lignin, and rice straw hydrolysate samples. Further proof of concept studies with model compounds demonstrated the applicability of antibody-based binders as a detection tool for monitoring microbial LDM conversion. Overall, this study demonstrates the potential of scFv binders as a specific toolset for lignin compound recovery and analysis.",
keywords = "FERULIC ACID, ANTIOXIDANT ACTIVITY, CHEMICALS, VALORIZATION, BIOMASS, BIOREFINERY, ANTIBODY, WASTE, PURIFICATION",
author = "Milla Salmela and Hanna Sanmark and Elena Efimova and Alexander Efimov and Hyt{\"o}nen, {Vesa P.} and Urpo Lamminm{\"a}ki and Suvi Santala and Ville Santala",
year = "2018",
month = "6",
day = "21",
doi = "10.1039/c8gc00490k",
language = "English",
volume = "20",
pages = "2829--2839",
journal = "Green Chemistry",
issn = "1463-9262",
publisher = "ROYAL SOC CHEMISTRY",
number = "12",

}

RIS (suitable for import to EndNote) - Download

TY - JOUR

T1 - Molecular tools for selective recovery and detection of lignin-derived molecules

AU - Salmela, Milla

AU - Sanmark, Hanna

AU - Efimova, Elena

AU - Efimov, Alexander

AU - Hytönen, Vesa P.

AU - Lamminmäki, Urpo

AU - Santala, Suvi

AU - Santala, Ville

PY - 2018/6/21

Y1 - 2018/6/21

N2 - The pulp and paper industry together with lignocellulosic biofuel production provides plentiful streams of lignin and lignin-derived molecules (LDMs) that currently remain underutilized. The heterogeneity and complexity of lignin along with the lack of convenient tools significantly hamper its utilization. Selective separation of these LDMs from streams using specific tools would allow the recovery of aromatic compounds, as well as facilitate biological processes aiming at lignin valorization. To this end, here we report the isolation and characterization of single-chain variable fragment (scFv) antibodies against ferulate, coumarate, and caffeate, which are the molecular representatives of LDMs. Binders for the target LDMs were enriched by interrogating a synthetic scFv library with the phage display technique. As a result, scFv binders specific against each of the target molecules were obtained with affinities in the micromolar range. The selectivity of scFvs towards specific LDMs was proved by recovering caffeate from simulated LDM solution, Kraft lignin, and rice straw hydrolysate samples. Further proof of concept studies with model compounds demonstrated the applicability of antibody-based binders as a detection tool for monitoring microbial LDM conversion. Overall, this study demonstrates the potential of scFv binders as a specific toolset for lignin compound recovery and analysis.

AB - The pulp and paper industry together with lignocellulosic biofuel production provides plentiful streams of lignin and lignin-derived molecules (LDMs) that currently remain underutilized. The heterogeneity and complexity of lignin along with the lack of convenient tools significantly hamper its utilization. Selective separation of these LDMs from streams using specific tools would allow the recovery of aromatic compounds, as well as facilitate biological processes aiming at lignin valorization. To this end, here we report the isolation and characterization of single-chain variable fragment (scFv) antibodies against ferulate, coumarate, and caffeate, which are the molecular representatives of LDMs. Binders for the target LDMs were enriched by interrogating a synthetic scFv library with the phage display technique. As a result, scFv binders specific against each of the target molecules were obtained with affinities in the micromolar range. The selectivity of scFvs towards specific LDMs was proved by recovering caffeate from simulated LDM solution, Kraft lignin, and rice straw hydrolysate samples. Further proof of concept studies with model compounds demonstrated the applicability of antibody-based binders as a detection tool for monitoring microbial LDM conversion. Overall, this study demonstrates the potential of scFv binders as a specific toolset for lignin compound recovery and analysis.

KW - FERULIC ACID

KW - ANTIOXIDANT ACTIVITY

KW - CHEMICALS

KW - VALORIZATION

KW - BIOMASS

KW - BIOREFINERY

KW - ANTIBODY

KW - WASTE

KW - PURIFICATION

U2 - 10.1039/c8gc00490k

DO - 10.1039/c8gc00490k

M3 - Article

VL - 20

SP - 2829

EP - 2839

JO - Green Chemistry

JF - Green Chemistry

SN - 1463-9262

IS - 12

ER -