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Non-sterile process for biohydrogen and 1,3-propanediol production from raw glycerol

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Original languageEnglish
Pages (from-to)11749-11755
Number of pages7
JournalInternational Journal of Hydrogen Energy
Issue number27
Publication statusPublished - 10 Sep 2013
Publication typeA1 Journal article-refereed


Raw glycerol is a tempting substrate for fermentations, but contains impurities that can be inhibitory for organisms. In this study, raw glycerol tolerance and contamination risk of pure bacterial culture at hypersaline process conditions were evaluated. The inhibitory effect of raw glycerol was similar on a halophilic (Halanaerobium saccharolyticum) and a non-halophilic (Clostridium butyricum) bacterium implying the inhibition originating from methanol or other impurities rather than salt. The hypersaline process conditions decreased efficiently contaminations and no growth of contaminants was observed at and above 125 g/l NaCl. Halophilic H2 and 1,3-PD production from raw glycerol were studied separately as 1-stage processes and jointly as 2-stage process in non-sterile conditions. Non-sterile conditions were successfully applied and the highest production yields obtained were 3.0 mol H2/mol glycerol and 0.66 mol 1,3-PD/mol glycerol (1-stage processes), whereas the highest cumulative production was 74 mmol H 2/l culture and 31 mmol 1,3-PD/l culture (2-stage process). © 2013, Hydrogen Energy Publications, LLC. Published by Elsevier Ltd. All rights.


  • Biodiesel by-product, Clostridium, Contamination risk, Halanaerobium, Halophilic fermentation, Raw glycerol tolerance

Publication forum classification

Field of science, Statistics Finland