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O2-requiring molecular reporters of gene expression for anaerobic microorganisms

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O2-requiring molecular reporters of gene expression for anaerobic microorganisms. / Guglielmetti, Simone; Santala, Ville; Mangayil, Rahul; Ciranna, Alessandro; Karp, Matti T.

In: Biosensors and Bioelectronics, Vol. 123, 2019, p. 1-6.

Research output: Contribution to journalArticleScientificpeer-review

Harvard

Guglielmetti, S, Santala, V, Mangayil, R, Ciranna, A & Karp, MT 2019, 'O2-requiring molecular reporters of gene expression for anaerobic microorganisms', Biosensors and Bioelectronics, vol. 123, pp. 1-6. https://doi.org/10.1016/j.bios.2018.09.066

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Author

Guglielmetti, Simone ; Santala, Ville ; Mangayil, Rahul ; Ciranna, Alessandro ; Karp, Matti T. / O2-requiring molecular reporters of gene expression for anaerobic microorganisms. In: Biosensors and Bioelectronics. 2019 ; Vol. 123. pp. 1-6.

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@article{468c7261829849fbac38f4dbc395a1bd,
title = "O2-requiring molecular reporters of gene expression for anaerobic microorganisms",
abstract = "Many genetic reporter systems require molecular oxygen; therefore, the use of reporter genes to study molecular mechanisms in anaerobic microorganisms has been hampered by the lack of convenient reporting systems. We describe reporter gene whole cell-based biosensor systems based on luciferase genes and the associated oxygen-requiring enzymes. By using two different oxygen-dependent reporters, insect and bacterial luciferases, and two bacterial hosts, Gram (+) Bifidobacterium longum and Gram (-) Escherichia coli, we show that the enzymes can be used in gene expression studies of anaerobic bacteria. E. coli, a facultative anaerobe, was grown both in aerobic and anaerobic conditions with an arabinose-inducible expression system. We show that a short treatment time of few minutes in ambient atmosphere is sufficient to detect light emission from living cells that is directly proportional to the number of cells and to the inducer concentration. The induction levels were the same in both the aerobically and anaerobically cultured cells. Similar results were obtained in the case of B. longum cultured in anaerobic conditions.",
keywords = "Bioluminescence, Gram (+), Gram (-), Luciferase, Arabinose induction",
author = "Simone Guglielmetti and Ville Santala and Rahul Mangayil and Alessandro Ciranna and Karp, {Matti T.}",
year = "2019",
doi = "10.1016/j.bios.2018.09.066",
language = "English",
volume = "123",
pages = "1--6",
journal = "Biosensors and Bioelectronics",
issn = "0956-5663",
publisher = "Elsevier",

}

RIS (suitable for import to EndNote) - Download

TY - JOUR

T1 - O2-requiring molecular reporters of gene expression for anaerobic microorganisms

AU - Guglielmetti, Simone

AU - Santala, Ville

AU - Mangayil, Rahul

AU - Ciranna, Alessandro

AU - Karp, Matti T.

PY - 2019

Y1 - 2019

N2 - Many genetic reporter systems require molecular oxygen; therefore, the use of reporter genes to study molecular mechanisms in anaerobic microorganisms has been hampered by the lack of convenient reporting systems. We describe reporter gene whole cell-based biosensor systems based on luciferase genes and the associated oxygen-requiring enzymes. By using two different oxygen-dependent reporters, insect and bacterial luciferases, and two bacterial hosts, Gram (+) Bifidobacterium longum and Gram (-) Escherichia coli, we show that the enzymes can be used in gene expression studies of anaerobic bacteria. E. coli, a facultative anaerobe, was grown both in aerobic and anaerobic conditions with an arabinose-inducible expression system. We show that a short treatment time of few minutes in ambient atmosphere is sufficient to detect light emission from living cells that is directly proportional to the number of cells and to the inducer concentration. The induction levels were the same in both the aerobically and anaerobically cultured cells. Similar results were obtained in the case of B. longum cultured in anaerobic conditions.

AB - Many genetic reporter systems require molecular oxygen; therefore, the use of reporter genes to study molecular mechanisms in anaerobic microorganisms has been hampered by the lack of convenient reporting systems. We describe reporter gene whole cell-based biosensor systems based on luciferase genes and the associated oxygen-requiring enzymes. By using two different oxygen-dependent reporters, insect and bacterial luciferases, and two bacterial hosts, Gram (+) Bifidobacterium longum and Gram (-) Escherichia coli, we show that the enzymes can be used in gene expression studies of anaerobic bacteria. E. coli, a facultative anaerobe, was grown both in aerobic and anaerobic conditions with an arabinose-inducible expression system. We show that a short treatment time of few minutes in ambient atmosphere is sufficient to detect light emission from living cells that is directly proportional to the number of cells and to the inducer concentration. The induction levels were the same in both the aerobically and anaerobically cultured cells. Similar results were obtained in the case of B. longum cultured in anaerobic conditions.

KW - Bioluminescence, Gram (+), Gram (-), Luciferase, Arabinose induction

U2 - 10.1016/j.bios.2018.09.066

DO - 10.1016/j.bios.2018.09.066

M3 - Article

VL - 123

SP - 1

EP - 6

JO - Biosensors and Bioelectronics

JF - Biosensors and Bioelectronics

SN - 0956-5663

ER -