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Optical projection tomography imaging of single cells in 3D gellan gum hydrogel

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Optical projection tomography imaging of single cells in 3D gellan gum hydrogel. / Belay, B.; Koivisto, J. T.; Vuornos, K.; Montonen, T.; Koskela, O.; Lehti-Polojärvi, M.; Miettinen, S.; Kellomäki, M.; Figueiras, E.; Hyttinen, J.

EMBEC and NBC 2017 - Joint Conference of the European Medical and Biological Engineering Conference EMBEC 2017 and the Nordic-Baltic Conference on Biomedical Engineering and Medical Physics, NBC 2017. Springer Verlag, 2018. p. 996-999 (IFMBE Proceedings; Vol. 65).

Research output: Chapter in Book/Report/Conference proceedingConference contributionScientificpeer-review

Harvard

Belay, B, Koivisto, JT, Vuornos, K, Montonen, T, Koskela, O, Lehti-Polojärvi, M, Miettinen, S, Kellomäki, M, Figueiras, E & Hyttinen, J 2018, Optical projection tomography imaging of single cells in 3D gellan gum hydrogel. in EMBEC and NBC 2017 - Joint Conference of the European Medical and Biological Engineering Conference EMBEC 2017 and the Nordic-Baltic Conference on Biomedical Engineering and Medical Physics, NBC 2017. IFMBE Proceedings, vol. 65, Springer Verlag, pp. 996-999, Joint Conference of the European Medical and Biological Engineering Conference (EMBEC) and the Nordic-Baltic Conference on Biomedical Engineering and Medical Physics (NBC), 1/01/00. https://doi.org/10.1007/978-981-10-5122-7_249

APA

Belay, B., Koivisto, J. T., Vuornos, K., Montonen, T., Koskela, O., Lehti-Polojärvi, M., ... Hyttinen, J. (2018). Optical projection tomography imaging of single cells in 3D gellan gum hydrogel. In EMBEC and NBC 2017 - Joint Conference of the European Medical and Biological Engineering Conference EMBEC 2017 and the Nordic-Baltic Conference on Biomedical Engineering and Medical Physics, NBC 2017 (pp. 996-999). (IFMBE Proceedings; Vol. 65). Springer Verlag. https://doi.org/10.1007/978-981-10-5122-7_249

Vancouver

Belay B, Koivisto JT, Vuornos K, Montonen T, Koskela O, Lehti-Polojärvi M et al. Optical projection tomography imaging of single cells in 3D gellan gum hydrogel. In EMBEC and NBC 2017 - Joint Conference of the European Medical and Biological Engineering Conference EMBEC 2017 and the Nordic-Baltic Conference on Biomedical Engineering and Medical Physics, NBC 2017. Springer Verlag. 2018. p. 996-999. (IFMBE Proceedings). https://doi.org/10.1007/978-981-10-5122-7_249

Author

Belay, B. ; Koivisto, J. T. ; Vuornos, K. ; Montonen, T. ; Koskela, O. ; Lehti-Polojärvi, M. ; Miettinen, S. ; Kellomäki, M. ; Figueiras, E. ; Hyttinen, J. / Optical projection tomography imaging of single cells in 3D gellan gum hydrogel. EMBEC and NBC 2017 - Joint Conference of the European Medical and Biological Engineering Conference EMBEC 2017 and the Nordic-Baltic Conference on Biomedical Engineering and Medical Physics, NBC 2017. Springer Verlag, 2018. pp. 996-999 (IFMBE Proceedings).

Bibtex - Download

@inproceedings{f3b351f4b42c4481aa2c2b714535d344,
title = "Optical projection tomography imaging of single cells in 3D gellan gum hydrogel",
abstract = "3D cell culturing has become attractive in biology and tissue engineering laboratories as it mimics the natural environment for the cells to grow, differentiate and interact in all directions. To study cells and cellular interactions within 3D, cell culture requires a non-invasive, non-toxic, and high resolution imaging technique. The existing imaging techniques face challenges to image cells in 3D macro-scale environment because of the sample size, photo-bleaching or resolution requirements. Optical projection tomography (OPT) is a non-invasive 3D imaging technique for samples in the range of 1-10 mm. It works in both emission and transmission modes for fluorescence and bright-field imaging, respectively. Here, we demonstrate the use of OPT for imaging of cells and cellular materials in 3D gellan gum hydrogel. Fluorescence projection images showed alive and dead human lung fibroblast cells encapsulated in hydrogel. The mineralized extracellular matrix secreted by the human adipose stem cells in the hydrogel was evenly distributed throughout the sample and analyzable in 3D volume.",
keywords = "3D imaging, Fluorescence, Hydrogel, Mesenchymal cell culture, Optical projection tomography",
author = "B. Belay and Koivisto, {J. T.} and K. Vuornos and T. Montonen and O. Koskela and M. Lehti-Poloj{\"a}rvi and S. Miettinen and M. Kellom{\"a}ki and E. Figueiras and J. Hyttinen",
note = "jufoid=58152 EXT={"}Vuornos, K.{"}",
year = "2018",
doi = "10.1007/978-981-10-5122-7_249",
language = "English",
isbn = "9789811051210",
series = "IFMBE Proceedings",
publisher = "Springer Verlag",
pages = "996--999",
booktitle = "EMBEC and NBC 2017 - Joint Conference of the European Medical and Biological Engineering Conference EMBEC 2017 and the Nordic-Baltic Conference on Biomedical Engineering and Medical Physics, NBC 2017",
address = "Germany",

}

RIS (suitable for import to EndNote) - Download

TY - GEN

T1 - Optical projection tomography imaging of single cells in 3D gellan gum hydrogel

AU - Belay, B.

AU - Koivisto, J. T.

AU - Vuornos, K.

AU - Montonen, T.

AU - Koskela, O.

AU - Lehti-Polojärvi, M.

AU - Miettinen, S.

AU - Kellomäki, M.

AU - Figueiras, E.

AU - Hyttinen, J.

N1 - jufoid=58152 EXT="Vuornos, K."

PY - 2018

Y1 - 2018

N2 - 3D cell culturing has become attractive in biology and tissue engineering laboratories as it mimics the natural environment for the cells to grow, differentiate and interact in all directions. To study cells and cellular interactions within 3D, cell culture requires a non-invasive, non-toxic, and high resolution imaging technique. The existing imaging techniques face challenges to image cells in 3D macro-scale environment because of the sample size, photo-bleaching or resolution requirements. Optical projection tomography (OPT) is a non-invasive 3D imaging technique for samples in the range of 1-10 mm. It works in both emission and transmission modes for fluorescence and bright-field imaging, respectively. Here, we demonstrate the use of OPT for imaging of cells and cellular materials in 3D gellan gum hydrogel. Fluorescence projection images showed alive and dead human lung fibroblast cells encapsulated in hydrogel. The mineralized extracellular matrix secreted by the human adipose stem cells in the hydrogel was evenly distributed throughout the sample and analyzable in 3D volume.

AB - 3D cell culturing has become attractive in biology and tissue engineering laboratories as it mimics the natural environment for the cells to grow, differentiate and interact in all directions. To study cells and cellular interactions within 3D, cell culture requires a non-invasive, non-toxic, and high resolution imaging technique. The existing imaging techniques face challenges to image cells in 3D macro-scale environment because of the sample size, photo-bleaching or resolution requirements. Optical projection tomography (OPT) is a non-invasive 3D imaging technique for samples in the range of 1-10 mm. It works in both emission and transmission modes for fluorescence and bright-field imaging, respectively. Here, we demonstrate the use of OPT for imaging of cells and cellular materials in 3D gellan gum hydrogel. Fluorescence projection images showed alive and dead human lung fibroblast cells encapsulated in hydrogel. The mineralized extracellular matrix secreted by the human adipose stem cells in the hydrogel was evenly distributed throughout the sample and analyzable in 3D volume.

KW - 3D imaging

KW - Fluorescence

KW - Hydrogel

KW - Mesenchymal cell culture

KW - Optical projection tomography

U2 - 10.1007/978-981-10-5122-7_249

DO - 10.1007/978-981-10-5122-7_249

M3 - Conference contribution

SN - 9789811051210

T3 - IFMBE Proceedings

SP - 996

EP - 999

BT - EMBEC and NBC 2017 - Joint Conference of the European Medical and Biological Engineering Conference EMBEC 2017 and the Nordic-Baltic Conference on Biomedical Engineering and Medical Physics, NBC 2017

PB - Springer Verlag

ER -