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Pneumatically actuated elastomeric device for simultaneous mechanobiological studies & live-cell fluorescent microscopy

Research output: Chapter in Book/Report/Conference proceedingConference contributionScientificpeer-review

Details

Original languageEnglish
Title of host publicationInternational Conference on Manipulation, Automation and Robotics at Small Scales, MARSS 2017 - Proceedings
PublisherIEEE
ISBN (Electronic)9781538603468
DOIs
Publication statusPublished - 3 Aug 2017
Publication typeA4 Article in a conference publication
EventInternational Conference on Manipulation, Automation and Robotics at Small Scales (MARSS) -
Duration: 1 Jan 2000 → …

Conference

ConferenceInternational Conference on Manipulation, Automation and Robotics at Small Scales (MARSS)
Period1/01/00 → …

Abstract

In this study, we demonstrate the functionality and usability of a compact, pneumatically actuated, elastomeric stimulation device for mechanobiological studies. The soft mechatronic device enables high-resolution live-cell confocal fluorescent imaging during equiaxial stretching. Several single cells can be tracked and imaged repeatedly after stretching periods. For demonstration, we provide image based analysis of dynamic change of the cell body and the nucleus area and actin fiber orientation during mechanical stimulation of mouse embryonic fibroblast (MEF) cells. Additionally, we present the characteristics of the device utilizing computational simulations and experimental validation using a particle tracking method for strain field analysis.

Keywords

  • High-resolution imaging, Mechanical stimulation, Mouse embryonic fibroblasts, Particle tracking, PDMS, Pneumatic actuation, Strain field analysis

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