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Production of l-xylose from l-xylulose using Escherichia coli l-fucose isomerase

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Details

Original languageEnglish
Pages (from-to)71-76
Number of pages6
JournalENZYME AND MICROBIAL TECHNOLOGY
Volume50
Issue number1
DOIs
Publication statusPublished - 5 Jan 2012
Publication typeA1 Journal article-refereed

Abstract

l-Xylulose was used as a raw material for the production of l-xylose with a recombinantly produced Escherichia coli l-fucose isomerase as the catalyst. The enzyme had a very alkaline pH optimum (over 10.5) and displayed Michaelis-Menten kinetics for l-xylulose with a K m of 41mM and a V max of 0.23μmol/(mgmin). The half-lives determined for the enzyme at 35°C and at 45°C were 6h 50min and 1h 31min, respectively. The reaction equilibrium between l-xylulose and l-xylose was 15:85 at 35°C and thus favored the formation of l-xylose. Contrary to the l-rhamnose isomerase catalyzed reaction described previously [14] l-lyxose was not detected in the reaction mixture with l-fucose isomerase. Although xylitol acted as an inhibitor of the reaction, even at a high ratio of xylitol to l-xylulose the inhibition did not reach 50%.

Keywords

  • Isomerization, L-Fucose isomerase, L-Xylose, L-Xylulose, Rare sugars