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Rapid and sensitive detection of norovirus antibodies in human serum with a biolayer interferometry biosensor

Research output: Contribution to journalArticleScientificpeer-review

Details

Original languageEnglish
Pages (from-to)507-514
Number of pages8
JournalSensors and Actuators B: Chemical
Volume221
DOIs
Publication statusPublished - 31 Dec 2015
Publication typeA1 Journal article-refereed

Abstract

Here, we describe the use of a biolayer interferometry biosensor for the fast and sensitive detection of virus-specific antibodies from human serum samples. Norovirus-like particles and norovirus P-particles were used to functionalise the biosensor tip. The detection of antibodies directly from serum samples was challenging, but the addition of a metal chelator (DAB) combined with an anti-human horseradish peroxidase-tagged antibody enabled enhanced detection of virus-specific antibodies in serum dilutions up to 1:100,000. Biolayer interferometry provides results faster than an ELISA, with results in as little as 10-20 min when using pre-functionalised sensors. Therefore, biolayer interferometry combined with DAB enhancement offers an attractive method for quick and sensitive quantification of biomolecules from complicated sample matrices.

Keywords

  • Biolayer interferometry, Fast diagnostics, Non-labelled detection, Norovirus, P-particles, Virus-like particles (VLPs)