Rapid and sensitive detection of norovirus antibodies in human serum with a biolayer interferometry biosensor
Research output: Contribution to journal › Article › Scientific › peer-review
Details
| Original language | English |
|---|---|
| Pages (from-to) | 507-514 |
| Number of pages | 8 |
| Journal | Sensors and Actuators B: Chemical |
| Volume | 221 |
| DOIs | |
| Publication status | Published - 31 Dec 2015 |
| Publication type | A1 Journal article-refereed |
Abstract
Here, we describe the use of a biolayer interferometry biosensor for the fast and sensitive detection of virus-specific antibodies from human serum samples. Norovirus-like particles and norovirus P-particles were used to functionalise the biosensor tip. The detection of antibodies directly from serum samples was challenging, but the addition of a metal chelator (DAB) combined with an anti-human horseradish peroxidase-tagged antibody enabled enhanced detection of virus-specific antibodies in serum dilutions up to 1:100,000. Biolayer interferometry provides results faster than an ELISA, with results in as little as 10-20 min when using pre-functionalised sensors. Therefore, biolayer interferometry combined with DAB enhancement offers an attractive method for quick and sensitive quantification of biomolecules from complicated sample matrices.
ASJC Scopus subject areas
Keywords
- Biolayer interferometry, Fast diagnostics, Non-labelled detection, Norovirus, P-particles, Virus-like particles (VLPs)