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Redox-coupled proton transfer in the respiratory complex I

Research output: Other contributionScientificpeer-review

Details

Original languageEnglish
TypeConference abstract
Media of outputPoster
PublisherEuropean Biophysics Journal
Volume44
Publication statusPublished - 2015

Abstract

Complex I functions as a redox-driven proton pump in the
aerobic respiratory chains of bacteria and eukaryotes. Re-
markably, the electron transfer domain of complex I is sepa-
rated by
ca
. 200
̊
A from the most distant proton pathways.
To understand the molecular mechanism of this biomolecular
machine, we perform here large-scale classical and quantum
molecular simulations, and site-directed mutagenesis experi-
ments, to study how the quinone reduction triggers the func-
tion of the proton pump. We find that coupled electrostatic,
conformational, and hydration changes are central for estab-
lishing the long-range energy coupling in complex I.