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Regulation of human mesenchymal stem cell osteogenesis by specific surface density of fibronectin: A gradient study

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Regulation of human mesenchymal stem cell osteogenesis by specific surface density of fibronectin : A gradient study. / Faia-Torres, Ana B.; Goren, Tolga; Ihalainen, Teemu O.; Guimond-Lischer, Stefanie; Charnley, Mirren; Rottmar, Markus; Maniura-Weber, Katharina; Spencer, Nicholas D.; Reis, Rui L.; Textor, Marcus; Neves, Nuno M.

In: ACS Applied Materials and Interfaces, Vol. 7, No. 4, 04.02.2015, p. 2367-2375.

Research output: Contribution to journalArticleScientificpeer-review

Harvard

Faia-Torres, AB, Goren, T, Ihalainen, TO, Guimond-Lischer, S, Charnley, M, Rottmar, M, Maniura-Weber, K, Spencer, ND, Reis, RL, Textor, M & Neves, NM 2015, 'Regulation of human mesenchymal stem cell osteogenesis by specific surface density of fibronectin: A gradient study', ACS Applied Materials and Interfaces, vol. 7, no. 4, pp. 2367-2375. https://doi.org/10.1021/am506951c

APA

Faia-Torres, A. B., Goren, T., Ihalainen, T. O., Guimond-Lischer, S., Charnley, M., Rottmar, M., ... Neves, N. M. (2015). Regulation of human mesenchymal stem cell osteogenesis by specific surface density of fibronectin: A gradient study. ACS Applied Materials and Interfaces, 7(4), 2367-2375. https://doi.org/10.1021/am506951c

Vancouver

Faia-Torres AB, Goren T, Ihalainen TO, Guimond-Lischer S, Charnley M, Rottmar M et al. Regulation of human mesenchymal stem cell osteogenesis by specific surface density of fibronectin: A gradient study. ACS Applied Materials and Interfaces. 2015 Feb 4;7(4):2367-2375. https://doi.org/10.1021/am506951c

Author

Faia-Torres, Ana B. ; Goren, Tolga ; Ihalainen, Teemu O. ; Guimond-Lischer, Stefanie ; Charnley, Mirren ; Rottmar, Markus ; Maniura-Weber, Katharina ; Spencer, Nicholas D. ; Reis, Rui L. ; Textor, Marcus ; Neves, Nuno M. / Regulation of human mesenchymal stem cell osteogenesis by specific surface density of fibronectin : A gradient study. In: ACS Applied Materials and Interfaces. 2015 ; Vol. 7, No. 4. pp. 2367-2375.

Bibtex - Download

@article{96668ddc5df44635a37774167421a2aa,
title = "Regulation of human mesenchymal stem cell osteogenesis by specific surface density of fibronectin: A gradient study",
abstract = "The success of synthetic bone implants requires good interface between the material and the host tissue. To study the biological relevance of fibronectin (FN) density on the osteogenic commitment of human bone marrow mesenchymal stem cells (hBM-MSCs), human FN was adsorbed in a linear density gradient on the surface of PCL. The evolution of the osteogenic markers alkaline phosphatase and collagen 1 alpha 1 was monitored by immunohistochemistry, and the cytoskeletal organization and the cell-derived FN were assessed. The functional analysis of the gradient revealed that the lower FN-density elicited stronger osteogenic expression and higher cytoskeleton spreading, hallmarks of the stem cell commitment to the osteoblastic lineage. The identification of the optimal FN density regime for the osteogenic commitment of hBM-MSCs presents a simple and versatile strategy to significantly enhance the surface properties of polycaprolactone as a paradigm for other synthetic polymers intended for bone-related applications.",
keywords = "biomaterials, fibronectin, gradient, mesenchymal stem cells, osteogenesis",
author = "Faia-Torres, {Ana B.} and Tolga Goren and Ihalainen, {Teemu O.} and Stefanie Guimond-Lischer and Mirren Charnley and Markus Rottmar and Katharina Maniura-Weber and Spencer, {Nicholas D.} and Reis, {Rui L.} and Marcus Textor and Neves, {Nuno M.}",
year = "2015",
month = "2",
day = "4",
doi = "10.1021/am506951c",
language = "English",
volume = "7",
pages = "2367--2375",
journal = "ACS Applied Materials & Interfaces",
issn = "1944-8244",
publisher = "American Chemical Society ACS",
number = "4",

}

RIS (suitable for import to EndNote) - Download

TY - JOUR

T1 - Regulation of human mesenchymal stem cell osteogenesis by specific surface density of fibronectin

T2 - A gradient study

AU - Faia-Torres, Ana B.

AU - Goren, Tolga

AU - Ihalainen, Teemu O.

AU - Guimond-Lischer, Stefanie

AU - Charnley, Mirren

AU - Rottmar, Markus

AU - Maniura-Weber, Katharina

AU - Spencer, Nicholas D.

AU - Reis, Rui L.

AU - Textor, Marcus

AU - Neves, Nuno M.

PY - 2015/2/4

Y1 - 2015/2/4

N2 - The success of synthetic bone implants requires good interface between the material and the host tissue. To study the biological relevance of fibronectin (FN) density on the osteogenic commitment of human bone marrow mesenchymal stem cells (hBM-MSCs), human FN was adsorbed in a linear density gradient on the surface of PCL. The evolution of the osteogenic markers alkaline phosphatase and collagen 1 alpha 1 was monitored by immunohistochemistry, and the cytoskeletal organization and the cell-derived FN were assessed. The functional analysis of the gradient revealed that the lower FN-density elicited stronger osteogenic expression and higher cytoskeleton spreading, hallmarks of the stem cell commitment to the osteoblastic lineage. The identification of the optimal FN density regime for the osteogenic commitment of hBM-MSCs presents a simple and versatile strategy to significantly enhance the surface properties of polycaprolactone as a paradigm for other synthetic polymers intended for bone-related applications.

AB - The success of synthetic bone implants requires good interface between the material and the host tissue. To study the biological relevance of fibronectin (FN) density on the osteogenic commitment of human bone marrow mesenchymal stem cells (hBM-MSCs), human FN was adsorbed in a linear density gradient on the surface of PCL. The evolution of the osteogenic markers alkaline phosphatase and collagen 1 alpha 1 was monitored by immunohistochemistry, and the cytoskeletal organization and the cell-derived FN were assessed. The functional analysis of the gradient revealed that the lower FN-density elicited stronger osteogenic expression and higher cytoskeleton spreading, hallmarks of the stem cell commitment to the osteoblastic lineage. The identification of the optimal FN density regime for the osteogenic commitment of hBM-MSCs presents a simple and versatile strategy to significantly enhance the surface properties of polycaprolactone as a paradigm for other synthetic polymers intended for bone-related applications.

KW - biomaterials

KW - fibronectin

KW - gradient

KW - mesenchymal stem cells

KW - osteogenesis

UR - http://www.scopus.com/inward/record.url?scp=84922438980&partnerID=8YFLogxK

U2 - 10.1021/am506951c

DO - 10.1021/am506951c

M3 - Article

VL - 7

SP - 2367

EP - 2375

JO - ACS Applied Materials & Interfaces

JF - ACS Applied Materials & Interfaces

SN - 1944-8244

IS - 4

ER -