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Robustness of the process of nucleoid exclusion of protein aggregates in Escherichia coli

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Robustness of the process of nucleoid exclusion of protein aggregates in Escherichia coli. / Neeli-Venkata, Ramakanth; Martikainen, Antti; Gupta, Abhishekh; Goncalves, Nadia; Fonseca, Jose; Ribeiro, Andre S.

In: Journal of Bacteriology, Vol. 198, No. 6, 2016, p. 898-906.

Research output: Contribution to journalArticleScientificpeer-review

Harvard

Neeli-Venkata, R, Martikainen, A, Gupta, A, Goncalves, N, Fonseca, J & Ribeiro, AS 2016, 'Robustness of the process of nucleoid exclusion of protein aggregates in Escherichia coli', Journal of Bacteriology, vol. 198, no. 6, pp. 898-906. https://doi.org/10.1128/JB.00848-15

APA

Neeli-Venkata, R., Martikainen, A., Gupta, A., Goncalves, N., Fonseca, J., & Ribeiro, A. S. (2016). Robustness of the process of nucleoid exclusion of protein aggregates in Escherichia coli. Journal of Bacteriology, 198(6), 898-906. https://doi.org/10.1128/JB.00848-15

Vancouver

Neeli-Venkata R, Martikainen A, Gupta A, Goncalves N, Fonseca J, Ribeiro AS. Robustness of the process of nucleoid exclusion of protein aggregates in Escherichia coli. Journal of Bacteriology. 2016;198(6):898-906. https://doi.org/10.1128/JB.00848-15

Author

Neeli-Venkata, Ramakanth ; Martikainen, Antti ; Gupta, Abhishekh ; Goncalves, Nadia ; Fonseca, Jose ; Ribeiro, Andre S. / Robustness of the process of nucleoid exclusion of protein aggregates in Escherichia coli. In: Journal of Bacteriology. 2016 ; Vol. 198, No. 6. pp. 898-906.

Bibtex - Download

@article{f9ed5a0e3b3644d5a3682b94c9d7beb3,
title = "Robustness of the process of nucleoid exclusion of protein aggregates in Escherichia coli",
abstract = "Escherichia coli segregates protein aggregates to the poles by nucleoid exclusion. Combined with cell divisions, this generates heterogeneous aggregate distributions in subsequent cell generations. We studied the robustness of this process with differing medium richness and antibiotics stress, which affect nucleoid size, using multimodal, time-lapse microscopy of live cells expressing both a fluorescently tagged chaperone (IbpA), which identifies in vivo the location of aggregates, and HupA-mCherry, a fluorescent variant of a nucleoid-associated protein. We find that the relative sizes of the nucleoid's major and minor axes change widely, in a positively correlated fashion, with medium richness and antibiotic stress. The aggregate's distribution along the major cell axis also changes between conditions and in agreement with the nucleoid exclusion phenomenon. Consequently, the fraction of aggregates at the midcell region prior to cell division differs between conditions, which will affect the degree of asymmetries in the partitioning of aggregates between cells of future generations. Finally, from the location of the peak of anisotropy in the aggregate displacement distribution, the nucleoid relative size, and the spatiotemporal aggregate distribution, we find that the exclusion of detectable aggregates from midcell is most pronounced in cells with mid-sized nucleoids, which are most common under optimal conditions. We conclude that the aggregate management mechanisms of E. coli are significantly robust but are not immune to stresses due to the tangible effect that these have on nucleoid size.",
author = "Ramakanth Neeli-Venkata and Antti Martikainen and Abhishekh Gupta and Nadia Goncalves and Jose Fonseca and Ribeiro, {Andre S.}",
note = "INT=sgn, {"}Martikainen, Antti{"}",
year = "2016",
doi = "10.1128/JB.00848-15",
language = "English",
volume = "198",
pages = "898--906",
journal = "Journal of Bacteriology",
issn = "0021-9193",
publisher = "American Society for Microbiology",
number = "6",

}

RIS (suitable for import to EndNote) - Download

TY - JOUR

T1 - Robustness of the process of nucleoid exclusion of protein aggregates in Escherichia coli

AU - Neeli-Venkata, Ramakanth

AU - Martikainen, Antti

AU - Gupta, Abhishekh

AU - Goncalves, Nadia

AU - Fonseca, Jose

AU - Ribeiro, Andre S.

N1 - INT=sgn, "Martikainen, Antti"

PY - 2016

Y1 - 2016

N2 - Escherichia coli segregates protein aggregates to the poles by nucleoid exclusion. Combined with cell divisions, this generates heterogeneous aggregate distributions in subsequent cell generations. We studied the robustness of this process with differing medium richness and antibiotics stress, which affect nucleoid size, using multimodal, time-lapse microscopy of live cells expressing both a fluorescently tagged chaperone (IbpA), which identifies in vivo the location of aggregates, and HupA-mCherry, a fluorescent variant of a nucleoid-associated protein. We find that the relative sizes of the nucleoid's major and minor axes change widely, in a positively correlated fashion, with medium richness and antibiotic stress. The aggregate's distribution along the major cell axis also changes between conditions and in agreement with the nucleoid exclusion phenomenon. Consequently, the fraction of aggregates at the midcell region prior to cell division differs between conditions, which will affect the degree of asymmetries in the partitioning of aggregates between cells of future generations. Finally, from the location of the peak of anisotropy in the aggregate displacement distribution, the nucleoid relative size, and the spatiotemporal aggregate distribution, we find that the exclusion of detectable aggregates from midcell is most pronounced in cells with mid-sized nucleoids, which are most common under optimal conditions. We conclude that the aggregate management mechanisms of E. coli are significantly robust but are not immune to stresses due to the tangible effect that these have on nucleoid size.

AB - Escherichia coli segregates protein aggregates to the poles by nucleoid exclusion. Combined with cell divisions, this generates heterogeneous aggregate distributions in subsequent cell generations. We studied the robustness of this process with differing medium richness and antibiotics stress, which affect nucleoid size, using multimodal, time-lapse microscopy of live cells expressing both a fluorescently tagged chaperone (IbpA), which identifies in vivo the location of aggregates, and HupA-mCherry, a fluorescent variant of a nucleoid-associated protein. We find that the relative sizes of the nucleoid's major and minor axes change widely, in a positively correlated fashion, with medium richness and antibiotic stress. The aggregate's distribution along the major cell axis also changes between conditions and in agreement with the nucleoid exclusion phenomenon. Consequently, the fraction of aggregates at the midcell region prior to cell division differs between conditions, which will affect the degree of asymmetries in the partitioning of aggregates between cells of future generations. Finally, from the location of the peak of anisotropy in the aggregate displacement distribution, the nucleoid relative size, and the spatiotemporal aggregate distribution, we find that the exclusion of detectable aggregates from midcell is most pronounced in cells with mid-sized nucleoids, which are most common under optimal conditions. We conclude that the aggregate management mechanisms of E. coli are significantly robust but are not immune to stresses due to the tangible effect that these have on nucleoid size.

U2 - 10.1128/JB.00848-15

DO - 10.1128/JB.00848-15

M3 - Article

VL - 198

SP - 898

EP - 906

JO - Journal of Bacteriology

JF - Journal of Bacteriology

SN - 0021-9193

IS - 6

ER -