Tampere University of Technology

TUTCRIS Research Portal

Silencing of the arp2/3 complex disturbs pancreatic cancer cell migration

Research output: Contribution to journalArticleScientificpeer-review


Original languageEnglish
Pages (from-to)45-52
Number of pages8
JournalAnticancer Research
Issue number1
Publication statusPublished - Jan 2013
Publication typeA1 Journal article-refereed


Background: Actin-related protein 2/3 (ARP2/3) complex is an actin nucleator responsible for actin cytoskeleton branching which is essential for efficient cell migration. Materials and Methods: The expression of the seven ARP2/3 complex subunits was assessed in pancreatic cancer cell lines and in normal pancreatic samples by quantitative RT-PCR. siRNA-mediated silencing was used to study the contribution of each ARP2/3 complex member to pancreatic cancer cell migration. Results: ARPC3 and ARPC4 were the most highly expressed complex members, while ARPC1B and ARPC2 were expressed at low levels. Silencing of the ARP2/3 complex subunits typically resulted in reduced cell migration capacity. In particular, silencing of ARPC4 significantly reduced cell migration in all studied cell lines, with a major impact on Hs700T and HPAFII migration (50% and 68% decrease, p

ASJC Scopus subject areas


  • ARP2/3 complex, Cell migration, Pancreatic cancer