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Silencing of the arp2/3 complex disturbs pancreatic cancer cell migration

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Details

Original languageEnglish
Pages (from-to)45-52
Number of pages8
JournalAnticancer Research
Volume33
Issue number1
Publication statusPublished - Jan 2013
Publication typeA1 Journal article-refereed

Abstract

Background: Actin-related protein 2/3 (ARP2/3) complex is an actin nucleator responsible for actin cytoskeleton branching which is essential for efficient cell migration. Materials and Methods: The expression of the seven ARP2/3 complex subunits was assessed in pancreatic cancer cell lines and in normal pancreatic samples by quantitative RT-PCR. siRNA-mediated silencing was used to study the contribution of each ARP2/3 complex member to pancreatic cancer cell migration. Results: ARPC3 and ARPC4 were the most highly expressed complex members, while ARPC1B and ARPC2 were expressed at low levels. Silencing of the ARP2/3 complex subunits typically resulted in reduced cell migration capacity. In particular, silencing of ARPC4 significantly reduced cell migration in all studied cell lines, with a major impact on Hs700T and HPAFII migration (50% and 68% decrease, p

ASJC Scopus subject areas

Keywords

  • ARP2/3 complex, Cell migration, Pancreatic cancer