Tampere University of Technology

TUTCRIS Research Portal

Simultaneous nutrient removal and lipid production with Chlorella vulgaris on sterilized and non-sterilized anaerobically pretreated piggery wastewater

Research output: Contribution to journalArticleScientificpeer-review


Original languageEnglish
Pages (from-to)177-184
Number of pages8
JournalBiochemical Engineering Journal
Early online date23 Jul 2015
Publication statusPublished - 5 Nov 2015
Publication typeA1 Journal article-refereed


Piggery wastewater is a potent nutrient source for microalgal lipid production. Wastewater has been usually sterilized when used for microalgal cultivation. This is uneconomical in large-scale applications. Therefore, lipid productivity of Chlorella vulgaris CY5 using sterilized and non-sterilized diluted anaerobically pretreated piggery wastewater was studied in batch reactors. The maximum average lipid productivity was obtained after 12 days of incubation and it was higher with the sterilized wastewater than with the non-sterilized one (117g/L/d vs. 91.3g/L/d), due to the higher biomass concentration. Because of the unexpected increase of dissolved organic carbon (DOC) in the cultures, second experiment was conducted to characterize the composition of produced DOC in non-sterilized wastewater. Carbohydrate content increased in the liquid phase but decreased in the biomass after nitrogen had been exhausted. After 12 days of incubation, soluble chemical oxygen demand (COD<inf>s</inf>) was 414±56mg/L, biomass production was 2.8±0.15g/L, and lipid content was 30.3±1.2wt%. Average lipid productivity from day zero to day 12 was 70.5±1.1g/L/d. C. vulgaris removed nutrients from the non-sterilized wastewater and produced oleaginous biomass, although the lipid productivity was higher with sterilized wastewater.


  • Aerobic process, Lipid production, Microalgae, Piggery wastewater, Sterilization, Wastewater treatment

Publication forum classification