Tampere University of Technology

TUTCRIS Research Portal

Soft hydrazone crosslinked hyaluronan- and alginate-based hydrogels as 3D supportive matrices for human pluripotent stem cell-derived neuronal cells

Research output: Contribution to journalArticleScientificpeer-review

Standard

Soft hydrazone crosslinked hyaluronan- and alginate-based hydrogels as 3D supportive matrices for human pluripotent stem cell-derived neuronal cells. / Karvinen, Jennika; Joki, Tiina; Ylä-Outinen, Laura; Koivisto, Janne T.; Narkilahti, Susanna; Kellomäki, Minna.

In: Reactive and Functional Polymers, Vol. 124, 01.03.2018, p. 29-39.

Research output: Contribution to journalArticleScientificpeer-review

Harvard

APA

Vancouver

Author

Karvinen, Jennika ; Joki, Tiina ; Ylä-Outinen, Laura ; Koivisto, Janne T. ; Narkilahti, Susanna ; Kellomäki, Minna. / Soft hydrazone crosslinked hyaluronan- and alginate-based hydrogels as 3D supportive matrices for human pluripotent stem cell-derived neuronal cells. In: Reactive and Functional Polymers. 2018 ; Vol. 124. pp. 29-39.

Bibtex - Download

@article{69ef8d0291e54be2aed5a9127ec313f5,
title = "Soft hydrazone crosslinked hyaluronan- and alginate-based hydrogels as 3D supportive matrices for human pluripotent stem cell-derived neuronal cells",
abstract = "Regenerative medicine, especially cell therapy combined with a supportive biomaterial scaffold, is considered to be a potential treatment for various deficits in humans. Here, we have produced and investigated the detailed properties of injectable hydrazone crosslinked hyaluronan-polyvinyl alcohol (HA-PVA) and alginate-polyvinyl alcohol (AL-PVA) hydrogels to be used as a supportive biomaterial for 3D neural cell cultures. To the best of our knowledge, this is the first time the polymerization and properties of hydrazone crosslinked AL-PVA hydrogel have been reported. The effect of the degree of substitution and molecular weight of the polymer components as well as the polymer concentration of the hydrogel on the swelling, degradation and mechanical properties of the hydrogels is reported. Furthermore, we studied the effect of the above parameters on the growth of human pluripotent stem cell-derived neuronal cells. The most neural cell supportive HA-PVA hydrogel was composed of high molecular weight HA component with brain-mimicking mechanical properties and decreased polymer concentration. AL-PVA hydrogel, with stiffness quite similar to brain tissue, was also shown to be similarly supportive. Neuronal spreading and 3D network formation was enhanced inside the softest hydrogels.",
keywords = "3D neuronal culture, Alginate, Hyaluronan, Hydrazone, Hydrogel",
author = "Jennika Karvinen and Tiina Joki and Laura Yl{\"a}-Outinen and Koivisto, {Janne T.} and Susanna Narkilahti and Minna Kellom{\"a}ki",
year = "2018",
month = "3",
day = "1",
doi = "10.1016/j.reactfunctpolym.2017.12.019",
language = "English",
volume = "124",
pages = "29--39",
journal = "Reactive and Functional Polymers",
issn = "1381-5148",
publisher = "Elsevier Science B.V.",

}

RIS (suitable for import to EndNote) - Download

TY - JOUR

T1 - Soft hydrazone crosslinked hyaluronan- and alginate-based hydrogels as 3D supportive matrices for human pluripotent stem cell-derived neuronal cells

AU - Karvinen, Jennika

AU - Joki, Tiina

AU - Ylä-Outinen, Laura

AU - Koivisto, Janne T.

AU - Narkilahti, Susanna

AU - Kellomäki, Minna

PY - 2018/3/1

Y1 - 2018/3/1

N2 - Regenerative medicine, especially cell therapy combined with a supportive biomaterial scaffold, is considered to be a potential treatment for various deficits in humans. Here, we have produced and investigated the detailed properties of injectable hydrazone crosslinked hyaluronan-polyvinyl alcohol (HA-PVA) and alginate-polyvinyl alcohol (AL-PVA) hydrogels to be used as a supportive biomaterial for 3D neural cell cultures. To the best of our knowledge, this is the first time the polymerization and properties of hydrazone crosslinked AL-PVA hydrogel have been reported. The effect of the degree of substitution and molecular weight of the polymer components as well as the polymer concentration of the hydrogel on the swelling, degradation and mechanical properties of the hydrogels is reported. Furthermore, we studied the effect of the above parameters on the growth of human pluripotent stem cell-derived neuronal cells. The most neural cell supportive HA-PVA hydrogel was composed of high molecular weight HA component with brain-mimicking mechanical properties and decreased polymer concentration. AL-PVA hydrogel, with stiffness quite similar to brain tissue, was also shown to be similarly supportive. Neuronal spreading and 3D network formation was enhanced inside the softest hydrogels.

AB - Regenerative medicine, especially cell therapy combined with a supportive biomaterial scaffold, is considered to be a potential treatment for various deficits in humans. Here, we have produced and investigated the detailed properties of injectable hydrazone crosslinked hyaluronan-polyvinyl alcohol (HA-PVA) and alginate-polyvinyl alcohol (AL-PVA) hydrogels to be used as a supportive biomaterial for 3D neural cell cultures. To the best of our knowledge, this is the first time the polymerization and properties of hydrazone crosslinked AL-PVA hydrogel have been reported. The effect of the degree of substitution and molecular weight of the polymer components as well as the polymer concentration of the hydrogel on the swelling, degradation and mechanical properties of the hydrogels is reported. Furthermore, we studied the effect of the above parameters on the growth of human pluripotent stem cell-derived neuronal cells. The most neural cell supportive HA-PVA hydrogel was composed of high molecular weight HA component with brain-mimicking mechanical properties and decreased polymer concentration. AL-PVA hydrogel, with stiffness quite similar to brain tissue, was also shown to be similarly supportive. Neuronal spreading and 3D network formation was enhanced inside the softest hydrogels.

KW - 3D neuronal culture

KW - Alginate

KW - Hyaluronan

KW - Hydrazone

KW - Hydrogel

UR - http://www.scopus.com/inward/record.url?scp=85040229275&partnerID=8YFLogxK

U2 - 10.1016/j.reactfunctpolym.2017.12.019

DO - 10.1016/j.reactfunctpolym.2017.12.019

M3 - Article

VL - 124

SP - 29

EP - 39

JO - Reactive and Functional Polymers

JF - Reactive and Functional Polymers

SN - 1381-5148

ER -