Solar Fuels and Chemicals: Engineering Bacterial Platform for the Production of Long-Chain Hydrocarbons from Carbon Dioxide and Electricity
Research output: Book/Report › Doctoral thesis
|Publisher||Tampere University of Technology|
|Number of pages||75|
|State||Published - 23 Nov 2018|
|Publication type||G5 Doctoral dissertation (article)|
|Name||Tampere University of Technology. Publication|
In this study, a two-stage bacterial platform for the production of long-chain hydrocarbons from carbon dioxide was developed. In the first stage, acetogenic bacteria produce acetate from carbon dioxide and electricity as the sole sources of carbon and energy. In the second stage, the acetate is converted to long-chain hydrocarbons by a second bacterium, Acinetobacter baylyi. The platform is modular in nature, and different acetate-producing and -consuming processes can be combined. The final product can be determined and the production enhanced by genetically engineering A. baylyi.
Fatty aldehydes are specific intermediates in the biosynthesis of wax esters and alkanes. Fatty acyl-CoA reductases (FARs) are key enzymes in the production of fatty aldehydes. The natural fatty aldehyde production of A. baylyi was improved by overexpressing either native or heterologous FARs. The overexpression led to increased fatty aldehyde production, and enabled more efficient wax ester or alkane production, depending on the downstream pathway utilized. For the wax ester production, the native wax ester production pathway of A. baylyi, combined with the overexpression of the native FAR or a previously uncharacterized, putative reductase from Nevskia ramosa, was employed. The overexpression of either of these reductases led to increased wax ester production, resulting in the highest wax ester titers reported thus far for microbial production systems. A. baylyi does not naturally produce alkanes, but the expression of a two-step cyanobacterial pathway consisting of aldehyde- and alkane producing enzymes led to alkane synthesis. Furthermore, the natural ability of A. baylyi for alkane degradation was removed, thereby converting the natural alkane degrader into alkane producer.
The development of efficient production strains by metabolic engineering is restricted by the lack of sensitive and specific measurement methods for the target compounds. Intracellular biosensors may offer convenient means for high-throughput screening and optimization of the production strains. In order to study heterologous alkane biosynthesis in A. baylyi, an in vivo alkane biosensor working on two levels was developed. First, a gene encoding the green fluorescent protein (GFP) was placed under an alkane-inducible promoter, linking the alkane production to the expression of GFP. The second level is provided by a constantly expressed bacterial luciferase LuxAB, which utilizes the fatty aldehyde intermediate as a substrate in a reaction that produces visible light. Thus, the production of fatty aldehydes can be monitored with the luminescence signal. The two-level configuration of the sensor enabled the screening of different alkane-producing pathways, and the optimization of the expression levels of the pathway enzymes in order to improve the alkane biosynthesis in A. baylyi.
In summary, the production of wax esters from CO2 and electricity, as well as long-chain alkanes from CO2 and H2 were demonstrated with the two-stage bacterial platform. In addition, heterologous alkane biosynthesis was demonstrated in A. baylyi, and the natural wax ester production of A. baylyi was improved by metabolic engineering. Even though the platform holds potential for the sustainable production of drop-in fuels and chemicals, significant improvements in the production efficiency are required before the potential can be realized.