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Spontaneous and mechanically induced Ca2+ activity changes in hESC‐RPE cells during maturation

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Spontaneous and mechanically induced Ca2+ activity changes in hESC‐RPE cells during maturation. / Abu Khamidakh, Amna; Juuti-Uusitalo, Kati; Caetano dos Santos, Florentino; Skottman, Heli; Hyttinen, Jari.

In: Acta Ophthalmologica, Vol. 93, No. S225, 01.10.2015.

Research output: Contribution to journalMeeting AbstractScientificpeer-review

Harvard

Abu Khamidakh, A, Juuti-Uusitalo, K, Caetano dos Santos, F, Skottman, H & Hyttinen, J 2015, 'Spontaneous and mechanically induced Ca2+ activity changes in hESC‐RPE cells during maturation', Acta Ophthalmologica, vol. 93, no. S225.

APA

Abu Khamidakh, A., Juuti-Uusitalo, K., Caetano dos Santos, F., Skottman, H., & Hyttinen, J. (2015). Spontaneous and mechanically induced Ca2+ activity changes in hESC‐RPE cells during maturation. Acta Ophthalmologica, 93(S225).

Vancouver

Abu Khamidakh A, Juuti-Uusitalo K, Caetano dos Santos F, Skottman H, Hyttinen J. Spontaneous and mechanically induced Ca2+ activity changes in hESC‐RPE cells during maturation. Acta Ophthalmologica. 2015 Oct 1;93(S225).

Author

Abu Khamidakh, Amna ; Juuti-Uusitalo, Kati ; Caetano dos Santos, Florentino ; Skottman, Heli ; Hyttinen, Jari. / Spontaneous and mechanically induced Ca2+ activity changes in hESC‐RPE cells during maturation. In: Acta Ophthalmologica. 2015 ; Vol. 93, No. S225.

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@article{2cb806feb7854e84b17eb14f8974b5ab,
title = "Spontaneous and mechanically induced Ca2+ activity changes in hESC‐RPE cells during maturation",
abstract = "PurposeThe aim of this study was to investigate changes in spontaneous Ca2+ activity and mechanically induced intercellular Ca2+ communication in human embryonic stem cells-derived retinal pigment epithelium (hESC-RPE) during maturation.MethodsIn this study, we assessed Ca2+ activity in hESC-RPE cells cultured for 9 or 28 days. Ca2+ imaging was done using a Ca2+-sensitive fluorescence dye fluo-4-AM. Spontaneous Ca2+ activity and mechanically induced intercellular Ca2+ communication were recorded in control conditions, in the absence of extracellular Ca2+, after depletion of intracellular Ca2+ stores with thapsigargin, in presence of a gap junction blocker α-glycyrrhetinic acid and a P2-receptor blocker suramin.Results9 days cells exhibited twice lower spontaneous Ca2+ activity and 4-fold higher mechanically induced intercellular Ca2+ communication compared to 28 days cells. Absence of extracellular Ca2+ reduced spontaneous Ca2+ activity in 9 days cells and almost completely inhibited it in 28 days cells, while having no effect on mechanically induced intercellular Ca2+ communication. Depletion of intracellular Ca2+ stores abolished spontaneous Ca2+ activity in 9 days and 28 days cells, as well as mechanically induced intercellular Ca2+ communication in 28 days cells, while not affecting the latter in 9 days cells. Blockade of gap junctions and P2-receptors had no effect on spontaneous Ca2+ activity or mechanically induced intercellular Ca2+ communication in cells from both time points.ConclusionsOur results show that hESC-RPE cells undergo significant Ca2+ signaling re-arrangements during maturation: the cells increase spontaneous Ca2+ activity, while decreasing their intercellular Ca2+ communication.",
author = "{Abu Khamidakh}, Amna and Kati Juuti-Uusitalo and {Caetano dos Santos}, Florentino and Heli Skottman and Jari Hyttinen",
year = "2015",
month = "10",
day = "1",
language = "English",
volume = "93",
journal = "Acta Ophthalmologica",
issn = "1755-375X",
publisher = "Wiley",
number = "S225",

}

RIS (suitable for import to EndNote) - Download

TY - JOUR

T1 - Spontaneous and mechanically induced Ca2+ activity changes in hESC‐RPE cells during maturation

AU - Abu Khamidakh, Amna

AU - Juuti-Uusitalo, Kati

AU - Caetano dos Santos, Florentino

AU - Skottman, Heli

AU - Hyttinen, Jari

PY - 2015/10/1

Y1 - 2015/10/1

N2 - PurposeThe aim of this study was to investigate changes in spontaneous Ca2+ activity and mechanically induced intercellular Ca2+ communication in human embryonic stem cells-derived retinal pigment epithelium (hESC-RPE) during maturation.MethodsIn this study, we assessed Ca2+ activity in hESC-RPE cells cultured for 9 or 28 days. Ca2+ imaging was done using a Ca2+-sensitive fluorescence dye fluo-4-AM. Spontaneous Ca2+ activity and mechanically induced intercellular Ca2+ communication were recorded in control conditions, in the absence of extracellular Ca2+, after depletion of intracellular Ca2+ stores with thapsigargin, in presence of a gap junction blocker α-glycyrrhetinic acid and a P2-receptor blocker suramin.Results9 days cells exhibited twice lower spontaneous Ca2+ activity and 4-fold higher mechanically induced intercellular Ca2+ communication compared to 28 days cells. Absence of extracellular Ca2+ reduced spontaneous Ca2+ activity in 9 days cells and almost completely inhibited it in 28 days cells, while having no effect on mechanically induced intercellular Ca2+ communication. Depletion of intracellular Ca2+ stores abolished spontaneous Ca2+ activity in 9 days and 28 days cells, as well as mechanically induced intercellular Ca2+ communication in 28 days cells, while not affecting the latter in 9 days cells. Blockade of gap junctions and P2-receptors had no effect on spontaneous Ca2+ activity or mechanically induced intercellular Ca2+ communication in cells from both time points.ConclusionsOur results show that hESC-RPE cells undergo significant Ca2+ signaling re-arrangements during maturation: the cells increase spontaneous Ca2+ activity, while decreasing their intercellular Ca2+ communication.

AB - PurposeThe aim of this study was to investigate changes in spontaneous Ca2+ activity and mechanically induced intercellular Ca2+ communication in human embryonic stem cells-derived retinal pigment epithelium (hESC-RPE) during maturation.MethodsIn this study, we assessed Ca2+ activity in hESC-RPE cells cultured for 9 or 28 days. Ca2+ imaging was done using a Ca2+-sensitive fluorescence dye fluo-4-AM. Spontaneous Ca2+ activity and mechanically induced intercellular Ca2+ communication were recorded in control conditions, in the absence of extracellular Ca2+, after depletion of intracellular Ca2+ stores with thapsigargin, in presence of a gap junction blocker α-glycyrrhetinic acid and a P2-receptor blocker suramin.Results9 days cells exhibited twice lower spontaneous Ca2+ activity and 4-fold higher mechanically induced intercellular Ca2+ communication compared to 28 days cells. Absence of extracellular Ca2+ reduced spontaneous Ca2+ activity in 9 days cells and almost completely inhibited it in 28 days cells, while having no effect on mechanically induced intercellular Ca2+ communication. Depletion of intracellular Ca2+ stores abolished spontaneous Ca2+ activity in 9 days and 28 days cells, as well as mechanically induced intercellular Ca2+ communication in 28 days cells, while not affecting the latter in 9 days cells. Blockade of gap junctions and P2-receptors had no effect on spontaneous Ca2+ activity or mechanically induced intercellular Ca2+ communication in cells from both time points.ConclusionsOur results show that hESC-RPE cells undergo significant Ca2+ signaling re-arrangements during maturation: the cells increase spontaneous Ca2+ activity, while decreasing their intercellular Ca2+ communication.

M3 - Meeting Abstract

VL - 93

JO - Acta Ophthalmologica

JF - Acta Ophthalmologica

SN - 1755-375X

IS - S225

ER -