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Superquenching of SYBRGreen dye fluorescence in complex with DNA by gold nanoparticles

Research output: Contribution to journalArticleScientificpeer-review


Original languageEnglish
Pages (from-to)363-367
Number of pages5
Issue number6
Publication statusPublished - Nov 2012
Publication typeA1 Journal article-refereed


The influence of gold nanoparticles (diameter of about 2.5 nm) on the complex between the SYBRGreen dye and double-stranded DNA in solutions has been investigated by fluorescence spectroscopy. Strong quenching of dye fluorescence by nanosized gold particles ("superquenching"), characterized by a high Stern-Volmer constant of KSV = 3.3 × 107 L/mol, has been found. The superquenching effect in the test system is explained in terms of contribution of several processes: electron transfer, formation of aggregates of gold nanoparticles involving dye dications, and enhancement of intersystem crossing by a heavy atom (gold atoms of nanoparticles).

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