Superquenching of SYBRGreen dye fluorescence in complex with DNA by gold nanoparticles
Research output: Contribution to journal › Article › Scientific › peer-review
|Number of pages||5|
|Journal||HIGH ENERGY CHEMISTRY|
|Publication status||Published - Nov 2012|
|Publication type||A1 Journal article-refereed|
The influence of gold nanoparticles (diameter of about 2.5 nm) on the complex between the SYBRGreen dye and double-stranded DNA in solutions has been investigated by fluorescence spectroscopy. Strong quenching of dye fluorescence by nanosized gold particles ("superquenching"), characterized by a high Stern-Volmer constant of KSV = 3.3 × 107 L/mol, has been found. The superquenching effect in the test system is explained in terms of contribution of several processes: electron transfer, formation of aggregates of gold nanoparticles involving dye dications, and enhancement of intersystem crossing by a heavy atom (gold atoms of nanoparticles).