Switchavidin: Reversible biotin-avidin-biotin bridges with high affinity and specificity
Research output: Contribution to journal › Article › Scientific › peer-review
Details
Original language | English |
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Pages (from-to) | 2233-2243 |
Number of pages | 11 |
Journal | Bioconjugate Chemistry |
Volume | 25 |
Issue number | 12 |
DOIs | |
Publication status | Published - 17 Dec 2014 |
Publication type | A1 Journal article-refereed |
Abstract
Switchavidin is a chicken avidin mutant displaying reversible binding to biotin, an improved binding affinity toward conjugated biotin, and low nonspecific binding due to reduced surface charge. These properties make switchavidin an optimal tool in biosensor applications for the reversible immobilization of biotinylated proteins on biotinylated sensor surfaces. Furthermore, switchavidin opens novel possibilities for patterning, purification, and labeling. (Graph Presented).