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Switchavidin: Reversible biotin-avidin-biotin bridges with high affinity and specificity

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Details

Original languageEnglish
Pages (from-to)2233-2243
Number of pages11
JournalBioconjugate Chemistry
Volume25
Issue number12
DOIs
Publication statusPublished - 17 Dec 2014
Publication typeA1 Journal article-refereed

Abstract

Switchavidin is a chicken avidin mutant displaying reversible binding to biotin, an improved binding affinity toward conjugated biotin, and low nonspecific binding due to reduced surface charge. These properties make switchavidin an optimal tool in biosensor applications for the reversible immobilization of biotinylated proteins on biotinylated sensor surfaces. Furthermore, switchavidin opens novel possibilities for patterning, purification, and labeling. (Graph Presented).