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Temperature dependence of leakiness of transcription repression mechanisms of Escherichia coli

Research output: Chapter in Book/Report/Conference proceedingConference contributionScientificpeer-review

Details

Original languageEnglish
Title of host publicationComputational Methods in Systems Biology - 14th International Conference, CMSB 2016
PublisherSpringer Verlag
Pages341-342
Number of pages2
ISBN (Print)9783319451763
Publication statusPublished - 2016
Publication typeA4 Article in a conference publication
EventInternational Conference on Computational Methods in Systems Biology -
Duration: 1 Jan 1900 → …

Publication series

NameLecture Notes in Computer Science
Volume9859
ISSN (Print)0302-9743
ISSN (Electronic)1611-3349

Conference

ConferenceInternational Conference on Computational Methods in Systems Biology
Period1/01/00 → …

Abstract

In E. coli, transcription repression is essential in cellular functioning. However, its failure rates are non-negligible. We measured the leakiness rate of lacO3O1 promoter with single RNA sensitivity and its temperature dependence in live cells. After finding strong temperature dependence, we dissected the causes. While RNA polymerase numbers and kt, the rate of active transcription, vary weakly with temperature, the repression strength (dependent on number of repressors and binding and unbinding rates of repressors to the promoter) is heavily temperature dependent. We conclude that the lacO3O1 leakiness at low temperatures increases as the repression mechanism’s efficiency hampers.

Keywords

  • lacO3O1, Leakiness, MS2-GFP RNA detection, Repression, Time-lapse confocal microscopy, Transcription

Publication forum classification

Field of science, Statistics Finland