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Thermophilic versus mesophilic dark fermentation in xylose-fed fluidised bed reactors: Biohydrogen production and active microbial community

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Thermophilic versus mesophilic dark fermentation in xylose-fed fluidised bed reactors : Biohydrogen production and active microbial community. / Dessì, Paolo; Porca, Estefania; Waters, Nicholas R.; Lakaniemi, Aino-Maija; Collins, Gavin; Lens, Piet N.L.

In: International Journal of Hydrogen Energy, Vol. 43, No. 11, 2018, p. 5473-5485.

Research output: Contribution to journalArticleScientificpeer-review

Harvard

Dessì, P, Porca, E, Waters, NR, Lakaniemi, A-M, Collins, G & Lens, PNL 2018, 'Thermophilic versus mesophilic dark fermentation in xylose-fed fluidised bed reactors: Biohydrogen production and active microbial community', International Journal of Hydrogen Energy, vol. 43, no. 11, pp. 5473-5485. https://doi.org/10.1016/j.ijhydene.2018.01.158

APA

Dessì, P., Porca, E., Waters, N. R., Lakaniemi, A-M., Collins, G., & Lens, P. N. L. (2018). Thermophilic versus mesophilic dark fermentation in xylose-fed fluidised bed reactors: Biohydrogen production and active microbial community. International Journal of Hydrogen Energy, 43(11), 5473-5485. https://doi.org/10.1016/j.ijhydene.2018.01.158

Vancouver

Author

Dessì, Paolo ; Porca, Estefania ; Waters, Nicholas R. ; Lakaniemi, Aino-Maija ; Collins, Gavin ; Lens, Piet N.L. / Thermophilic versus mesophilic dark fermentation in xylose-fed fluidised bed reactors : Biohydrogen production and active microbial community. In: International Journal of Hydrogen Energy. 2018 ; Vol. 43, No. 11. pp. 5473-5485.

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@article{3de3d63bfef440c1b09c9b36bf849914,
title = "Thermophilic versus mesophilic dark fermentation in xylose-fed fluidised bed reactors: Biohydrogen production and active microbial community",
abstract = "Dark fermentative biohydrogen production in a thermophilic, xylose-fed (50 mM) fluidised bed reactor (FBR) was evaluated in the temperature range 55-70 °C with 5-degree increments and compared with a mesophilic FBR operated constantly at 37 °C. A significantly higher (p = 0.05) H2 yield was obtained in the thermophilic FBR, which stabilised at about 1.2 mol H2 mol-1 xylose (36{\%} of the theoretical maximum) at 55 and 70 °C, and at 0.8 mol H2 mol-1 xylose at 60 and 65 °C, compared to the mesophilic FBR (0.5 mol H2 mol-1 xylose). High-throughput sequencing of the reverse-transcribed 16S rRNA, done for the first time on biohydrogen producing reactors, indicated that Thermoanaerobacterium was the prevalent active microorganism in the thermophilic FBR, regardless of the operating temperature. The active microbial community in the mesophilic FBR was mainly composed of Clostridium and Ruminiclostridium at 37 °C. Thermophilic dark fermentation was shown to be suitable for treatment of high temperature, xylose-containing wastewaters, as it resulted in a higher energy output compared to the mesophilic counterpart.",
keywords = "Active community, Biohydrogen, FBR, MiSeq, Thermoanaerobacterium, Thermophilic",
author = "Paolo Dess{\`i} and Estefania Porca and Waters, {Nicholas R.} and Aino-Maija Lakaniemi and Gavin Collins and Lens, {Piet N.L.}",
year = "2018",
doi = "10.1016/j.ijhydene.2018.01.158",
language = "English",
volume = "43",
pages = "5473--5485",
journal = "International Journal of Hydrogen Energy",
issn = "0360-3199",
publisher = "Elsevier",
number = "11",

}

RIS (suitable for import to EndNote) - Download

TY - JOUR

T1 - Thermophilic versus mesophilic dark fermentation in xylose-fed fluidised bed reactors

T2 - Biohydrogen production and active microbial community

AU - Dessì, Paolo

AU - Porca, Estefania

AU - Waters, Nicholas R.

AU - Lakaniemi, Aino-Maija

AU - Collins, Gavin

AU - Lens, Piet N.L.

PY - 2018

Y1 - 2018

N2 - Dark fermentative biohydrogen production in a thermophilic, xylose-fed (50 mM) fluidised bed reactor (FBR) was evaluated in the temperature range 55-70 °C with 5-degree increments and compared with a mesophilic FBR operated constantly at 37 °C. A significantly higher (p = 0.05) H2 yield was obtained in the thermophilic FBR, which stabilised at about 1.2 mol H2 mol-1 xylose (36% of the theoretical maximum) at 55 and 70 °C, and at 0.8 mol H2 mol-1 xylose at 60 and 65 °C, compared to the mesophilic FBR (0.5 mol H2 mol-1 xylose). High-throughput sequencing of the reverse-transcribed 16S rRNA, done for the first time on biohydrogen producing reactors, indicated that Thermoanaerobacterium was the prevalent active microorganism in the thermophilic FBR, regardless of the operating temperature. The active microbial community in the mesophilic FBR was mainly composed of Clostridium and Ruminiclostridium at 37 °C. Thermophilic dark fermentation was shown to be suitable for treatment of high temperature, xylose-containing wastewaters, as it resulted in a higher energy output compared to the mesophilic counterpart.

AB - Dark fermentative biohydrogen production in a thermophilic, xylose-fed (50 mM) fluidised bed reactor (FBR) was evaluated in the temperature range 55-70 °C with 5-degree increments and compared with a mesophilic FBR operated constantly at 37 °C. A significantly higher (p = 0.05) H2 yield was obtained in the thermophilic FBR, which stabilised at about 1.2 mol H2 mol-1 xylose (36% of the theoretical maximum) at 55 and 70 °C, and at 0.8 mol H2 mol-1 xylose at 60 and 65 °C, compared to the mesophilic FBR (0.5 mol H2 mol-1 xylose). High-throughput sequencing of the reverse-transcribed 16S rRNA, done for the first time on biohydrogen producing reactors, indicated that Thermoanaerobacterium was the prevalent active microorganism in the thermophilic FBR, regardless of the operating temperature. The active microbial community in the mesophilic FBR was mainly composed of Clostridium and Ruminiclostridium at 37 °C. Thermophilic dark fermentation was shown to be suitable for treatment of high temperature, xylose-containing wastewaters, as it resulted in a higher energy output compared to the mesophilic counterpart.

KW - Active community

KW - Biohydrogen

KW - FBR

KW - MiSeq

KW - Thermoanaerobacterium

KW - Thermophilic

U2 - 10.1016/j.ijhydene.2018.01.158

DO - 10.1016/j.ijhydene.2018.01.158

M3 - Article

VL - 43

SP - 5473

EP - 5485

JO - International Journal of Hydrogen Energy

JF - International Journal of Hydrogen Energy

SN - 0360-3199

IS - 11

ER -