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Three-Dimensional Microstructured Azobenzene-Containing Gelatin as a Photoactuable Cell Confining System

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Three-Dimensional Microstructured Azobenzene-Containing Gelatin as a Photoactuable Cell Confining System. / Pennacchio, Fabrizio A.; Fedele, Chiara; De Martino, Selene; Cavalli, Silvia; Vecchione, Raffaele; Netti, Paolo A.

In: ACS Applied Materials and Interfaces, Vol. 10, No. 1, 10.01.2018, p. 91-97.

Research output: Contribution to journalArticleScientificpeer-review

Harvard

Pennacchio, FA, Fedele, C, De Martino, S, Cavalli, S, Vecchione, R & Netti, PA 2018, 'Three-Dimensional Microstructured Azobenzene-Containing Gelatin as a Photoactuable Cell Confining System', ACS Applied Materials and Interfaces, vol. 10, no. 1, pp. 91-97. https://doi.org/10.1021/acsami.7b13176

APA

Pennacchio, F. A., Fedele, C., De Martino, S., Cavalli, S., Vecchione, R., & Netti, P. A. (2018). Three-Dimensional Microstructured Azobenzene-Containing Gelatin as a Photoactuable Cell Confining System. ACS Applied Materials and Interfaces, 10(1), 91-97. https://doi.org/10.1021/acsami.7b13176

Vancouver

Pennacchio FA, Fedele C, De Martino S, Cavalli S, Vecchione R, Netti PA. Three-Dimensional Microstructured Azobenzene-Containing Gelatin as a Photoactuable Cell Confining System. ACS Applied Materials and Interfaces. 2018 Jan 10;10(1):91-97. https://doi.org/10.1021/acsami.7b13176

Author

Pennacchio, Fabrizio A. ; Fedele, Chiara ; De Martino, Selene ; Cavalli, Silvia ; Vecchione, Raffaele ; Netti, Paolo A. / Three-Dimensional Microstructured Azobenzene-Containing Gelatin as a Photoactuable Cell Confining System. In: ACS Applied Materials and Interfaces. 2018 ; Vol. 10, No. 1. pp. 91-97.

Bibtex - Download

@article{cd5aa4e12bd049d9915772b02e0d3b7e,
title = "Three-Dimensional Microstructured Azobenzene-Containing Gelatin as a Photoactuable Cell Confining System",
abstract = "In materials science, there is a considerable interest in the fabrication of highly engineered biomaterials that can interact with cells and control their shape. In particular, from the literature, the role played by physical cell confinement in cellular structural organization and thus in the regulation of its functions has been well-established. In this context, the addition of a dynamic feature to physically confining platforms aiming at reproducing in vitro the well-known dynamic interaction between the cells and their microenvironment would be highly desirable. To this aim, we have developed an advanced gelatin-based hydrogel that can be finely micropatterned by two-photon polymerization and stimulated in a controlled way by light irradiation thanks to the presence of an azobenzene cross-linker. Light-triggered expansion of gelatin microstructures induced an in-plane nuclear deformation of physically confined NIH-3T3 cells. The microfabricated photoactuable gelatin shown in this work paves the way to new {"}dynamic{"} caging culture systems that can find applications, for example, as {"}engineered stem cell niches{"}.",
keywords = "azobenzene, cell confinement, hydrogel, photoactuation, two-photon lithography",
author = "Pennacchio, {Fabrizio A.} and Chiara Fedele and {De Martino}, Selene and Silvia Cavalli and Raffaele Vecchione and Netti, {Paolo A.}",
note = "EXT={"}Fedele, Chiara{"}",
year = "2018",
month = "1",
day = "10",
doi = "10.1021/acsami.7b13176",
language = "English",
volume = "10",
pages = "91--97",
journal = "ACS Applied Materials & Interfaces",
issn = "1944-8244",
publisher = "American Chemical Society ACS",
number = "1",

}

RIS (suitable for import to EndNote) - Download

TY - JOUR

T1 - Three-Dimensional Microstructured Azobenzene-Containing Gelatin as a Photoactuable Cell Confining System

AU - Pennacchio, Fabrizio A.

AU - Fedele, Chiara

AU - De Martino, Selene

AU - Cavalli, Silvia

AU - Vecchione, Raffaele

AU - Netti, Paolo A.

N1 - EXT="Fedele, Chiara"

PY - 2018/1/10

Y1 - 2018/1/10

N2 - In materials science, there is a considerable interest in the fabrication of highly engineered biomaterials that can interact with cells and control their shape. In particular, from the literature, the role played by physical cell confinement in cellular structural organization and thus in the regulation of its functions has been well-established. In this context, the addition of a dynamic feature to physically confining platforms aiming at reproducing in vitro the well-known dynamic interaction between the cells and their microenvironment would be highly desirable. To this aim, we have developed an advanced gelatin-based hydrogel that can be finely micropatterned by two-photon polymerization and stimulated in a controlled way by light irradiation thanks to the presence of an azobenzene cross-linker. Light-triggered expansion of gelatin microstructures induced an in-plane nuclear deformation of physically confined NIH-3T3 cells. The microfabricated photoactuable gelatin shown in this work paves the way to new "dynamic" caging culture systems that can find applications, for example, as "engineered stem cell niches".

AB - In materials science, there is a considerable interest in the fabrication of highly engineered biomaterials that can interact with cells and control their shape. In particular, from the literature, the role played by physical cell confinement in cellular structural organization and thus in the regulation of its functions has been well-established. In this context, the addition of a dynamic feature to physically confining platforms aiming at reproducing in vitro the well-known dynamic interaction between the cells and their microenvironment would be highly desirable. To this aim, we have developed an advanced gelatin-based hydrogel that can be finely micropatterned by two-photon polymerization and stimulated in a controlled way by light irradiation thanks to the presence of an azobenzene cross-linker. Light-triggered expansion of gelatin microstructures induced an in-plane nuclear deformation of physically confined NIH-3T3 cells. The microfabricated photoactuable gelatin shown in this work paves the way to new "dynamic" caging culture systems that can find applications, for example, as "engineered stem cell niches".

KW - azobenzene

KW - cell confinement

KW - hydrogel

KW - photoactuation

KW - two-photon lithography

U2 - 10.1021/acsami.7b13176

DO - 10.1021/acsami.7b13176

M3 - Article

VL - 10

SP - 91

EP - 97

JO - ACS Applied Materials & Interfaces

JF - ACS Applied Materials & Interfaces

SN - 1944-8244

IS - 1

ER -