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Transbilayer lipid interactions mediate nanoclustering of lipid-anchored proteins

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Transbilayer lipid interactions mediate nanoclustering of lipid-anchored proteins. / Raghupathy, Riya; Anilkumar, Anupama Ambika; Polley, Anirban; Singh, Parvinder Pal; Yadav, Mahipal; Johnson, Charles; Suryawanshi, Sharad; Saikam, Varma; Sawant, Sanghapal D.; Panda, Aniruddha; Guo, Zhongwu; Vishwakarma, Ram A.; Rao, Madan; Mayor, Satyajit.

In: Cell, Vol. 161, No. 3, 23.04.2015, p. 581-594.

Research output: Contribution to journalArticleScientificpeer-review

Harvard

Raghupathy, R, Anilkumar, AA, Polley, A, Singh, PP, Yadav, M, Johnson, C, Suryawanshi, S, Saikam, V, Sawant, SD, Panda, A, Guo, Z, Vishwakarma, RA, Rao, M & Mayor, S 2015, 'Transbilayer lipid interactions mediate nanoclustering of lipid-anchored proteins', Cell, vol. 161, no. 3, pp. 581-594. https://doi.org/10.1016/j.cell.2015.03.048

APA

Raghupathy, R., Anilkumar, A. A., Polley, A., Singh, P. P., Yadav, M., Johnson, C., ... Mayor, S. (2015). Transbilayer lipid interactions mediate nanoclustering of lipid-anchored proteins. Cell, 161(3), 581-594. https://doi.org/10.1016/j.cell.2015.03.048

Vancouver

Raghupathy R, Anilkumar AA, Polley A, Singh PP, Yadav M, Johnson C et al. Transbilayer lipid interactions mediate nanoclustering of lipid-anchored proteins. Cell. 2015 Apr 23;161(3):581-594. https://doi.org/10.1016/j.cell.2015.03.048

Author

Raghupathy, Riya ; Anilkumar, Anupama Ambika ; Polley, Anirban ; Singh, Parvinder Pal ; Yadav, Mahipal ; Johnson, Charles ; Suryawanshi, Sharad ; Saikam, Varma ; Sawant, Sanghapal D. ; Panda, Aniruddha ; Guo, Zhongwu ; Vishwakarma, Ram A. ; Rao, Madan ; Mayor, Satyajit. / Transbilayer lipid interactions mediate nanoclustering of lipid-anchored proteins. In: Cell. 2015 ; Vol. 161, No. 3. pp. 581-594.

Bibtex - Download

@article{2d3ab2cfb8f94a308d67ae19189461a5,
title = "Transbilayer lipid interactions mediate nanoclustering of lipid-anchored proteins",
abstract = "Understanding how functional lipid domains in live cell membranes are generated has posed a challenge. Here, we show that transbilayer interactions are necessary for the generation of cholesterol-dependent nanoclusters of GPI-anchored proteins mediated by membrane-adjacent dynamic actin filaments. We find that long saturated acyl-chains are required for forming GPI-anchor nanoclusters. Simultaneously, at the inner leaflet, long acyl-chain-containing phosphatidylserine (PS) is necessary for transbilayer coupling. All-atom molecular dynamics simulations of asymmetric multicomponent-membrane bilayers in a mixed phase provide evidence that immobilization of long saturated acyl-chain lipids at either leaflet stabilizes cholesterol-dependent transbilayer interactions forming local domains with characteristics similar to a liquid-ordered (lo) phase. This is verified by experiments wherein immobilization of long acyl-chain lipids at one leaflet effects transbilayer interactions of corresponding lipids at the opposite leaflet. This suggests a general mechanism for the generation and stabilization of nanoscale cholesterol-dependent and actin-mediated lipid clusters in live cell membranes.",
author = "Riya Raghupathy and Anilkumar, {Anupama Ambika} and Anirban Polley and Singh, {Parvinder Pal} and Mahipal Yadav and Charles Johnson and Sharad Suryawanshi and Varma Saikam and Sawant, {Sanghapal D.} and Aniruddha Panda and Zhongwu Guo and Vishwakarma, {Ram A.} and Madan Rao and Satyajit Mayor",
year = "2015",
month = "4",
day = "23",
doi = "10.1016/j.cell.2015.03.048",
language = "English",
volume = "161",
pages = "581--594",
journal = "Cell",
issn = "0092-8674",
publisher = "Elsevier",
number = "3",

}

RIS (suitable for import to EndNote) - Download

TY - JOUR

T1 - Transbilayer lipid interactions mediate nanoclustering of lipid-anchored proteins

AU - Raghupathy, Riya

AU - Anilkumar, Anupama Ambika

AU - Polley, Anirban

AU - Singh, Parvinder Pal

AU - Yadav, Mahipal

AU - Johnson, Charles

AU - Suryawanshi, Sharad

AU - Saikam, Varma

AU - Sawant, Sanghapal D.

AU - Panda, Aniruddha

AU - Guo, Zhongwu

AU - Vishwakarma, Ram A.

AU - Rao, Madan

AU - Mayor, Satyajit

PY - 2015/4/23

Y1 - 2015/4/23

N2 - Understanding how functional lipid domains in live cell membranes are generated has posed a challenge. Here, we show that transbilayer interactions are necessary for the generation of cholesterol-dependent nanoclusters of GPI-anchored proteins mediated by membrane-adjacent dynamic actin filaments. We find that long saturated acyl-chains are required for forming GPI-anchor nanoclusters. Simultaneously, at the inner leaflet, long acyl-chain-containing phosphatidylserine (PS) is necessary for transbilayer coupling. All-atom molecular dynamics simulations of asymmetric multicomponent-membrane bilayers in a mixed phase provide evidence that immobilization of long saturated acyl-chain lipids at either leaflet stabilizes cholesterol-dependent transbilayer interactions forming local domains with characteristics similar to a liquid-ordered (lo) phase. This is verified by experiments wherein immobilization of long acyl-chain lipids at one leaflet effects transbilayer interactions of corresponding lipids at the opposite leaflet. This suggests a general mechanism for the generation and stabilization of nanoscale cholesterol-dependent and actin-mediated lipid clusters in live cell membranes.

AB - Understanding how functional lipid domains in live cell membranes are generated has posed a challenge. Here, we show that transbilayer interactions are necessary for the generation of cholesterol-dependent nanoclusters of GPI-anchored proteins mediated by membrane-adjacent dynamic actin filaments. We find that long saturated acyl-chains are required for forming GPI-anchor nanoclusters. Simultaneously, at the inner leaflet, long acyl-chain-containing phosphatidylserine (PS) is necessary for transbilayer coupling. All-atom molecular dynamics simulations of asymmetric multicomponent-membrane bilayers in a mixed phase provide evidence that immobilization of long saturated acyl-chain lipids at either leaflet stabilizes cholesterol-dependent transbilayer interactions forming local domains with characteristics similar to a liquid-ordered (lo) phase. This is verified by experiments wherein immobilization of long acyl-chain lipids at one leaflet effects transbilayer interactions of corresponding lipids at the opposite leaflet. This suggests a general mechanism for the generation and stabilization of nanoscale cholesterol-dependent and actin-mediated lipid clusters in live cell membranes.

UR - http://www.scopus.com/inward/record.url?scp=84928392356&partnerID=8YFLogxK

U2 - 10.1016/j.cell.2015.03.048

DO - 10.1016/j.cell.2015.03.048

M3 - Article

VL - 161

SP - 581

EP - 594

JO - Cell

JF - Cell

SN - 0092-8674

IS - 3

ER -