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Tuning extrinsic noise effects on a small genetic circuit

Research output: Chapter in Book/Report/Conference proceedingConference contributionScientificpeer-review

Details

Original languageEnglish
Title of host publicationProceedings of ECAL 2017
Subtitle of host publication14th European Conference on Artificial Life
PublisherMassachusetts Institute of Technology
ISBN (Electronic)978-0-262-34633-7
DOIs
Publication statusPublished - 4 Sep 2017
Publication typeA4 Article in a conference publication
EventEUROPEAN CONFERENCE ON ARTIFICIAL LIFE -
Duration: 1 Jan 1900 → …

Conference

ConferenceEUROPEAN CONFERENCE ON ARTIFICIAL LIFE
Period1/01/00 → …

Abstract

Measurements at the single cell level showed that monoclonal Escherichia coli cells differ widely in the numbers of components affecting gene expression dynamics. Using a stochastic model of a 2-genes symmetric toggle switch with realistic multi-step promoter initiation kinetics and empirically validated parameter values, we investigate the role of transcription initiation kinetics on the degree with which cell-to-cell variability in cellular components generates cell-to-cell diversity in switch dynamics. We find that while the mean switching frequency is determined by the promoter kinetics, the cell to cell diversity of this frequency depends both on promoter kinetics and diversity in RNA polymerase numbers. At a microscale level, the main regulator of the cell to cell variability in protein numbers (of both genes in ON and OFF states) is the promoters kinetics, not the diversity in RNA polymerase numbers. We conclude that the promoters kinetics is a critical regulator of the toggle switch dynamics and that can be used as a regulatable filter of extrinsic noise.

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Field of science, Statistics Finland

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