Tampere University of Technology

TUTCRIS Research Portal

Using a synthetic probe to study the robustness of the segregation process of protein aggregates in Escherichia coli

Research output: Chapter in Book/Report/Conference proceedingConference contributionScientificpeer-review

Details

Original languageEnglish
Title of host publicationThe 8th International Conference on Bioinformatics, Biocomputational Systems and Biotechnologies (BIOTECHNO2016)
PublisherIARIA
Pages21-22
ISBN (Electronic)978-1-61208-488-6
Publication statusPublished - 26 Jun 2016
Publication typeA4 Article in a conference publication
EventInternational Conference on Bioinformatics, Biocomputational Systems and Biotechnologies -
Duration: 1 Jan 2000 → …

Conference

ConferenceInternational Conference on Bioinformatics, Biocomputational Systems and Biotechnologies
Period1/01/00 → …

Abstract

Even though the processes of protein production and folding are not immune to errors, Escherichia coli lineages are capable to maintain a stable cell lineage, provided viable environmental conditions. One of the internal processes that makes this possible consists of segregating unwanted protein aggregates to the cell poles by nucleoid exclusion, which, combined with cell divisions, generates asymmetries in the aging process of the population, with some individuals aging faster while others exhibit rejuvenation. A recent study showed that this process is not immune to sub-optimal temperature conditions due to increased cytoplasm viscosity, which weakens the anisotropy in aggregate displacements at the nucleoid borders. This was made possible by the usage of a synthetic fluorescent probe, consisting of a RNA sequence with multiple binding sites for the MS2-GFP synthetic protein, which can be tracked in time-lapse microscopy images. Here we provide a description of the findings from these measurements and investigate with an In Silico model the consequences in the context of cell lineages.

Publication forum classification

Field of science, Statistics Finland