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Versatile bio-ink for covalent immobilization of chimeric avidin on sol-gel substrates

Research output: Contribution to journalArticleScientificpeer-review

Details

Original languageEnglish
Pages (from-to)409-414
Number of pages6
JournalColloids and Surfaces B: Biointerfaces
Volume87
Issue number2
DOIs
Publication statusPublished - 15 Oct 2011
Publication typeA1 Journal article-refereed

Abstract

A bio-ink for covalent deposition of thermostable, high affinity biotin-binding chimeric avidin onto sol-gel substrates was developed. The bio-ink was prepared from heterobifunctional crosslinker 6-maleimidohexanoic acid N-hydroxysuccinimide which was first reacted either with 3-aminopropyltriethoxysilane or 3-aminopropyldimethylethoxysilane to form silane linkers 6-maleimide- N-(3-(triethoxysilyl)propyl)hexanamide or -(ethoxydimethylsilyl)propyl)-hexanamide. C-terminal cysteine genetically engineered to chimeric avidin was reacted with the maleimide group of silane linker in methanol/PBS solution to form a suspension, which was printed on sol-gel modified PMMA film. Different concentrations of chimeric avidin and ratios between silane linkers were tested to find the best properties for the bio-ink to enable gravure or inkjet printing. Bio-ink prepared from 3-aminopropyltriethoxysilane was found to provide the highest amount of active immobilized chimeric avidin. The developed bio-ink was shown to be valuable for automated fabrication of avidin-functionalized polymer films.

Keywords

  • Avidin-biotin technology, Biomolecule immobilization, Biosensing, Chimeric avidin, Maleimide, Printing, Sol-gel