Versatile bio-ink for covalent immobilization of chimeric avidin on sol-gel substrates
Research output: Contribution to journal › Article › Scientific › peer-review
Details
| Original language | English |
|---|---|
| Pages (from-to) | 409-414 |
| Number of pages | 6 |
| Journal | Colloids and Surfaces B: Biointerfaces |
| Volume | 87 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 15 Oct 2011 |
| Publication type | A1 Journal article-refereed |
Abstract
A bio-ink for covalent deposition of thermostable, high affinity biotin-binding chimeric avidin onto sol-gel substrates was developed. The bio-ink was prepared from heterobifunctional crosslinker 6-maleimidohexanoic acid N-hydroxysuccinimide which was first reacted either with 3-aminopropyltriethoxysilane or 3-aminopropyldimethylethoxysilane to form silane linkers 6-maleimide- N-(3-(triethoxysilyl)propyl)hexanamide or -(ethoxydimethylsilyl)propyl)-hexanamide. C-terminal cysteine genetically engineered to chimeric avidin was reacted with the maleimide group of silane linker in methanol/PBS solution to form a suspension, which was printed on sol-gel modified PMMA film. Different concentrations of chimeric avidin and ratios between silane linkers were tested to find the best properties for the bio-ink to enable gravure or inkjet printing. Bio-ink prepared from 3-aminopropyltriethoxysilane was found to provide the highest amount of active immobilized chimeric avidin. The developed bio-ink was shown to be valuable for automated fabrication of avidin-functionalized polymer films.
ASJC Scopus subject areas
Keywords
- Avidin-biotin technology, Biomolecule immobilization, Biosensing, Chimeric avidin, Maleimide, Printing, Sol-gel