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Versatile bio-ink for covalent immobilization of chimeric avidin on sol-gel substrates

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Versatile bio-ink for covalent immobilization of chimeric avidin on sol-gel substrates. / Heikkinen, Jarkko J.; Kivimäki, Liisa; Määttä, Juha A E; Mäkelä, Inka; Hakalahti, Leena; Takkinen, Kristiina; Kulomaa, Markku S.; Hytönen, Vesa P.; Hormi, Osmo E O.

In: Colloids and Surfaces B: Biointerfaces, Vol. 87, No. 2, 15.10.2011, p. 409-414.

Research output: Contribution to journalArticleScientificpeer-review

Harvard

Heikkinen, JJ, Kivimäki, L, Määttä, JAE, Mäkelä, I, Hakalahti, L, Takkinen, K, Kulomaa, MS, Hytönen, VP & Hormi, OEO 2011, 'Versatile bio-ink for covalent immobilization of chimeric avidin on sol-gel substrates', Colloids and Surfaces B: Biointerfaces, vol. 87, no. 2, pp. 409-414. https://doi.org/10.1016/j.colsurfb.2011.05.052

APA

Heikkinen, J. J., Kivimäki, L., Määttä, J. A. E., Mäkelä, I., Hakalahti, L., Takkinen, K., ... Hormi, O. E. O. (2011). Versatile bio-ink for covalent immobilization of chimeric avidin on sol-gel substrates. Colloids and Surfaces B: Biointerfaces, 87(2), 409-414. https://doi.org/10.1016/j.colsurfb.2011.05.052

Vancouver

Heikkinen JJ, Kivimäki L, Määttä JAE, Mäkelä I, Hakalahti L, Takkinen K et al. Versatile bio-ink for covalent immobilization of chimeric avidin on sol-gel substrates. Colloids and Surfaces B: Biointerfaces. 2011 Oct 15;87(2):409-414. https://doi.org/10.1016/j.colsurfb.2011.05.052

Author

Heikkinen, Jarkko J. ; Kivimäki, Liisa ; Määttä, Juha A E ; Mäkelä, Inka ; Hakalahti, Leena ; Takkinen, Kristiina ; Kulomaa, Markku S. ; Hytönen, Vesa P. ; Hormi, Osmo E O. / Versatile bio-ink for covalent immobilization of chimeric avidin on sol-gel substrates. In: Colloids and Surfaces B: Biointerfaces. 2011 ; Vol. 87, No. 2. pp. 409-414.

Bibtex - Download

@article{ab00a0e238b1474997d1effcc0549eee,
title = "Versatile bio-ink for covalent immobilization of chimeric avidin on sol-gel substrates",
abstract = "A bio-ink for covalent deposition of thermostable, high affinity biotin-binding chimeric avidin onto sol-gel substrates was developed. The bio-ink was prepared from heterobifunctional crosslinker 6-maleimidohexanoic acid N-hydroxysuccinimide which was first reacted either with 3-aminopropyltriethoxysilane or 3-aminopropyldimethylethoxysilane to form silane linkers 6-maleimide- N-(3-(triethoxysilyl)propyl)hexanamide or -(ethoxydimethylsilyl)propyl)-hexanamide. C-terminal cysteine genetically engineered to chimeric avidin was reacted with the maleimide group of silane linker in methanol/PBS solution to form a suspension, which was printed on sol-gel modified PMMA film. Different concentrations of chimeric avidin and ratios between silane linkers were tested to find the best properties for the bio-ink to enable gravure or inkjet printing. Bio-ink prepared from 3-aminopropyltriethoxysilane was found to provide the highest amount of active immobilized chimeric avidin. The developed bio-ink was shown to be valuable for automated fabrication of avidin-functionalized polymer films.",
keywords = "Avidin-biotin technology, Biomolecule immobilization, Biosensing, Chimeric avidin, Maleimide, Printing, Sol-gel",
author = "Heikkinen, {Jarkko J.} and Liisa Kivim{\"a}ki and M{\"a}{\"a}tt{\"a}, {Juha A E} and Inka M{\"a}kel{\"a} and Leena Hakalahti and Kristiina Takkinen and Kulomaa, {Markku S.} and Hyt{\"o}nen, {Vesa P.} and Hormi, {Osmo E O}",
year = "2011",
month = "10",
day = "15",
doi = "10.1016/j.colsurfb.2011.05.052",
language = "English",
volume = "87",
pages = "409--414",
journal = "Colloids and Surfaces B: Biointerfaces",
issn = "0927-7765",
publisher = "Elsevier",
number = "2",

}

RIS (suitable for import to EndNote) - Download

TY - JOUR

T1 - Versatile bio-ink for covalent immobilization of chimeric avidin on sol-gel substrates

AU - Heikkinen, Jarkko J.

AU - Kivimäki, Liisa

AU - Määttä, Juha A E

AU - Mäkelä, Inka

AU - Hakalahti, Leena

AU - Takkinen, Kristiina

AU - Kulomaa, Markku S.

AU - Hytönen, Vesa P.

AU - Hormi, Osmo E O

PY - 2011/10/15

Y1 - 2011/10/15

N2 - A bio-ink for covalent deposition of thermostable, high affinity biotin-binding chimeric avidin onto sol-gel substrates was developed. The bio-ink was prepared from heterobifunctional crosslinker 6-maleimidohexanoic acid N-hydroxysuccinimide which was first reacted either with 3-aminopropyltriethoxysilane or 3-aminopropyldimethylethoxysilane to form silane linkers 6-maleimide- N-(3-(triethoxysilyl)propyl)hexanamide or -(ethoxydimethylsilyl)propyl)-hexanamide. C-terminal cysteine genetically engineered to chimeric avidin was reacted with the maleimide group of silane linker in methanol/PBS solution to form a suspension, which was printed on sol-gel modified PMMA film. Different concentrations of chimeric avidin and ratios between silane linkers were tested to find the best properties for the bio-ink to enable gravure or inkjet printing. Bio-ink prepared from 3-aminopropyltriethoxysilane was found to provide the highest amount of active immobilized chimeric avidin. The developed bio-ink was shown to be valuable for automated fabrication of avidin-functionalized polymer films.

AB - A bio-ink for covalent deposition of thermostable, high affinity biotin-binding chimeric avidin onto sol-gel substrates was developed. The bio-ink was prepared from heterobifunctional crosslinker 6-maleimidohexanoic acid N-hydroxysuccinimide which was first reacted either with 3-aminopropyltriethoxysilane or 3-aminopropyldimethylethoxysilane to form silane linkers 6-maleimide- N-(3-(triethoxysilyl)propyl)hexanamide or -(ethoxydimethylsilyl)propyl)-hexanamide. C-terminal cysteine genetically engineered to chimeric avidin was reacted with the maleimide group of silane linker in methanol/PBS solution to form a suspension, which was printed on sol-gel modified PMMA film. Different concentrations of chimeric avidin and ratios between silane linkers were tested to find the best properties for the bio-ink to enable gravure or inkjet printing. Bio-ink prepared from 3-aminopropyltriethoxysilane was found to provide the highest amount of active immobilized chimeric avidin. The developed bio-ink was shown to be valuable for automated fabrication of avidin-functionalized polymer films.

KW - Avidin-biotin technology

KW - Biomolecule immobilization

KW - Biosensing

KW - Chimeric avidin

KW - Maleimide

KW - Printing

KW - Sol-gel

UR - http://www.scopus.com/inward/record.url?scp=79960384544&partnerID=8YFLogxK

U2 - 10.1016/j.colsurfb.2011.05.052

DO - 10.1016/j.colsurfb.2011.05.052

M3 - Article

VL - 87

SP - 409

EP - 414

JO - Colloids and Surfaces B: Biointerfaces

JF - Colloids and Surfaces B: Biointerfaces

SN - 0927-7765

IS - 2

ER -