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Vertical stratification of bacteria and archaea in sediments of a small boreal humic lake

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Vertical stratification of bacteria and archaea in sediments of a small boreal humic lake. / Rissanen, Antti J.; Peura, Sari; Mpamah, Promise A.; Taipale, Sami; Tiirola, Marja; Biasi, Christina; Mäki, Anita; Nykänen, Hannu.

In: FEMS Microbiology Letters, Vol. 366, No. 5, 01.03.2019.

Research output: Contribution to journalArticleScientificpeer-review

Harvard

Rissanen, AJ, Peura, S, Mpamah, PA, Taipale, S, Tiirola, M, Biasi, C, Mäki, A & Nykänen, H 2019, 'Vertical stratification of bacteria and archaea in sediments of a small boreal humic lake', FEMS Microbiology Letters, vol. 366, no. 5. https://doi.org/10.1093/femsle/fnz044

APA

Rissanen, A. J., Peura, S., Mpamah, P. A., Taipale, S., Tiirola, M., Biasi, C., ... Nykänen, H. (2019). Vertical stratification of bacteria and archaea in sediments of a small boreal humic lake. FEMS Microbiology Letters, 366(5). https://doi.org/10.1093/femsle/fnz044

Vancouver

Rissanen AJ, Peura S, Mpamah PA, Taipale S, Tiirola M, Biasi C et al. Vertical stratification of bacteria and archaea in sediments of a small boreal humic lake. FEMS Microbiology Letters. 2019 Mar 1;366(5). https://doi.org/10.1093/femsle/fnz044

Author

Rissanen, Antti J. ; Peura, Sari ; Mpamah, Promise A. ; Taipale, Sami ; Tiirola, Marja ; Biasi, Christina ; Mäki, Anita ; Nykänen, Hannu. / Vertical stratification of bacteria and archaea in sediments of a small boreal humic lake. In: FEMS Microbiology Letters. 2019 ; Vol. 366, No. 5.

Bibtex - Download

@article{8dcfaab9f11740c197bf4a6aca004188,
title = "Vertical stratification of bacteria and archaea in sediments of a small boreal humic lake",
abstract = "Although sediments of small boreal humic lakes are important carbon stores and greenhouse gas sources, the composition and structuring mechanisms of their microbial communities have remained understudied. We analyzed the vertical profiles of microbial biomass indicators (PLFAs, DNA and RNA) and the bacterial and archaeal community composition (sequencing of 16S rRNA gene amplicons and qPCR of mcrA) in sediment cores collected from a typical small boreal lake. While microbial biomass decreased with sediment depth, viable microbes (RNA and PLFA) were present all through the profiles. The vertical stratification patterns of the bacterial and archaeal communities resembled those in marine sediments with well-characterized groups (e.g. Methanomicrobia, Proteobacteria, Cyanobacteria, Bacteroidetes) dominating in the surface sediment and being replaced by poorly-known groups (e.g. Bathyarchaeota, Aminicenantes and Caldiserica) in the deeper layers. The results also suggested that, similar to marine systems, the deep bacterial and archaeal communities were predominantly assembled by selective survival of taxa able to persist in the low energy conditions. Methanotrophs were rare, further corroborating the role of these methanogen-rich sediments as important methane emitters. Based on their taxonomy, the deep-dwelling groups were putatively organo-heterotrophic, organo-autotrophic and/or acetogenic and thus may contribute to changes in the lake sediment carbon storage.",
keywords = "16S rRNA, archaea, bacteria, biomass, lake, sediment",
author = "Rissanen, {Antti J.} and Sari Peura and Mpamah, {Promise A.} and Sami Taipale and Marja Tiirola and Christina Biasi and Anita M{\"a}ki and Hannu Nyk{\"a}nen",
year = "2019",
month = "3",
day = "1",
doi = "10.1093/femsle/fnz044",
language = "English",
volume = "366",
journal = "FEMS Microbiology Letters",
issn = "0378-1097",
publisher = "Oxford University Press",
number = "5",

}

RIS (suitable for import to EndNote) - Download

TY - JOUR

T1 - Vertical stratification of bacteria and archaea in sediments of a small boreal humic lake

AU - Rissanen, Antti J.

AU - Peura, Sari

AU - Mpamah, Promise A.

AU - Taipale, Sami

AU - Tiirola, Marja

AU - Biasi, Christina

AU - Mäki, Anita

AU - Nykänen, Hannu

PY - 2019/3/1

Y1 - 2019/3/1

N2 - Although sediments of small boreal humic lakes are important carbon stores and greenhouse gas sources, the composition and structuring mechanisms of their microbial communities have remained understudied. We analyzed the vertical profiles of microbial biomass indicators (PLFAs, DNA and RNA) and the bacterial and archaeal community composition (sequencing of 16S rRNA gene amplicons and qPCR of mcrA) in sediment cores collected from a typical small boreal lake. While microbial biomass decreased with sediment depth, viable microbes (RNA and PLFA) were present all through the profiles. The vertical stratification patterns of the bacterial and archaeal communities resembled those in marine sediments with well-characterized groups (e.g. Methanomicrobia, Proteobacteria, Cyanobacteria, Bacteroidetes) dominating in the surface sediment and being replaced by poorly-known groups (e.g. Bathyarchaeota, Aminicenantes and Caldiserica) in the deeper layers. The results also suggested that, similar to marine systems, the deep bacterial and archaeal communities were predominantly assembled by selective survival of taxa able to persist in the low energy conditions. Methanotrophs were rare, further corroborating the role of these methanogen-rich sediments as important methane emitters. Based on their taxonomy, the deep-dwelling groups were putatively organo-heterotrophic, organo-autotrophic and/or acetogenic and thus may contribute to changes in the lake sediment carbon storage.

AB - Although sediments of small boreal humic lakes are important carbon stores and greenhouse gas sources, the composition and structuring mechanisms of their microbial communities have remained understudied. We analyzed the vertical profiles of microbial biomass indicators (PLFAs, DNA and RNA) and the bacterial and archaeal community composition (sequencing of 16S rRNA gene amplicons and qPCR of mcrA) in sediment cores collected from a typical small boreal lake. While microbial biomass decreased with sediment depth, viable microbes (RNA and PLFA) were present all through the profiles. The vertical stratification patterns of the bacterial and archaeal communities resembled those in marine sediments with well-characterized groups (e.g. Methanomicrobia, Proteobacteria, Cyanobacteria, Bacteroidetes) dominating in the surface sediment and being replaced by poorly-known groups (e.g. Bathyarchaeota, Aminicenantes and Caldiserica) in the deeper layers. The results also suggested that, similar to marine systems, the deep bacterial and archaeal communities were predominantly assembled by selective survival of taxa able to persist in the low energy conditions. Methanotrophs were rare, further corroborating the role of these methanogen-rich sediments as important methane emitters. Based on their taxonomy, the deep-dwelling groups were putatively organo-heterotrophic, organo-autotrophic and/or acetogenic and thus may contribute to changes in the lake sediment carbon storage.

KW - 16S rRNA

KW - archaea

KW - bacteria

KW - biomass

KW - lake

KW - sediment

U2 - 10.1093/femsle/fnz044

DO - 10.1093/femsle/fnz044

M3 - Article

VL - 366

JO - FEMS Microbiology Letters

JF - FEMS Microbiology Letters

SN - 0378-1097

IS - 5

ER -