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Wound healing of human embryonic stem cell-derived retinal pigment epithelial cells is affected by maturation stage

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Wound healing of human embryonic stem cell-derived retinal pigment epithelial cells is affected by maturation stage. / Abu Khamidakh, Amna E.; Rodriguez-Martinez, Alejandra; Kaarniranta, Kai; Kallioniemi, Anne; Skottman, Heli; Hyttinen, Jari; Juuti-Uusitalo, Kati.

In: BioMedical Engineering Online, Vol. 17, No. 1, 102, 31.07.2018.

Research output: Contribution to journalArticleScientificpeer-review

Harvard

Abu Khamidakh, AE, Rodriguez-Martinez, A, Kaarniranta, K, Kallioniemi, A, Skottman, H, Hyttinen, J & Juuti-Uusitalo, K 2018, 'Wound healing of human embryonic stem cell-derived retinal pigment epithelial cells is affected by maturation stage', BioMedical Engineering Online, vol. 17, no. 1, 102. https://doi.org/10.1186/s12938-018-0535-z

APA

Abu Khamidakh, A. E., Rodriguez-Martinez, A., Kaarniranta, K., Kallioniemi, A., Skottman, H., Hyttinen, J., & Juuti-Uusitalo, K. (2018). Wound healing of human embryonic stem cell-derived retinal pigment epithelial cells is affected by maturation stage. BioMedical Engineering Online, 17(1), [102]. https://doi.org/10.1186/s12938-018-0535-z

Vancouver

Abu Khamidakh AE, Rodriguez-Martinez A, Kaarniranta K, Kallioniemi A, Skottman H, Hyttinen J et al. Wound healing of human embryonic stem cell-derived retinal pigment epithelial cells is affected by maturation stage. BioMedical Engineering Online. 2018 Jul 31;17(1). 102. https://doi.org/10.1186/s12938-018-0535-z

Author

Abu Khamidakh, Amna E. ; Rodriguez-Martinez, Alejandra ; Kaarniranta, Kai ; Kallioniemi, Anne ; Skottman, Heli ; Hyttinen, Jari ; Juuti-Uusitalo, Kati. / Wound healing of human embryonic stem cell-derived retinal pigment epithelial cells is affected by maturation stage. In: BioMedical Engineering Online. 2018 ; Vol. 17, No. 1.

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@article{551e0a39dc644538ab8e74b5482196e2,
title = "Wound healing of human embryonic stem cell-derived retinal pigment epithelial cells is affected by maturation stage",
abstract = "Background: Wound healing of retinal pigment epithelium (RPE) is a complex process that may take place in common age-related macular degeneration eye disease. The purpose of this study was to evaluate whether wounding and wound healing has an effect on Ca2+ dynamics in human embryonic stem cell (hESC)-RPEs cultured different periods of time. Methods: The 9-day-cultured or 28-day-cultured hESC-RPEs from two different cell lines were wounded and the dynamics of spontaneous and mechanically induced intracellular Ca2+ activity was measured with live-cell Ca2+ imaging either immediately or 7days after wounding. The healing time and speed were analyzed with time-lapse bright field microscopy. The Ca2+ activity and healing speed were analysed with image analysis. In addition the extracellular matrix deposition was assessed with confocal microscopy. Results: The Ca2+ dynamics in hESC-RPE monolayers differed depending on the culture time: 9-day-cultured cells had higher number of cells with spontaneous Ca2+ activity close to freshly wounded edge compared to control areas, whereas in 28-day-cultured cells there was no difference in wounded and control areas. The 28-day-cultured, wounded and 7-day-healed hESC-RPEs produced wide-spreading intercellular Ca2+ waves upon mechanical stimulation, while in controls propagation was restricted. Most importantly, both wave spreading and spontaneous Ca2+ activity of cells within the healed area, as well as the cell morphology of 28-day-cultured, wounded and thereafter 7-day-healed areas resembled the 9-day-cultured hESC-RPEs. Conclusions: This acquired knowledge about Ca2+ dynamics of wounded hESC-RPE monolayers is important for understanding the dynamics of RPE wound healing, and could offer a reliable functionality test for RPE cells. The data presented in here suggests that assessment of Ca2+ dynamics analysed with image analysis could be used as a reliable non-invasive functionality test for RPE cells.",
keywords = "Ca waves, Cell maturation, HESC-RPE, Image analysis, Mechanical stimulation, Mechanically induced intercellular Ca waves, RPE, Spontaneous [Ca] increases, Wound healing",
author = "{Abu Khamidakh}, {Amna E.} and Alejandra Rodriguez-Martinez and Kai Kaarniranta and Anne Kallioniemi and Heli Skottman and Jari Hyttinen and Kati Juuti-Uusitalo",
year = "2018",
month = "7",
day = "31",
doi = "10.1186/s12938-018-0535-z",
language = "English",
volume = "17",
journal = "BioMedical Engineering Online",
issn = "1475-925X",
publisher = "Springer Verlag",
number = "1",

}

RIS (suitable for import to EndNote) - Download

TY - JOUR

T1 - Wound healing of human embryonic stem cell-derived retinal pigment epithelial cells is affected by maturation stage

AU - Abu Khamidakh, Amna E.

AU - Rodriguez-Martinez, Alejandra

AU - Kaarniranta, Kai

AU - Kallioniemi, Anne

AU - Skottman, Heli

AU - Hyttinen, Jari

AU - Juuti-Uusitalo, Kati

PY - 2018/7/31

Y1 - 2018/7/31

N2 - Background: Wound healing of retinal pigment epithelium (RPE) is a complex process that may take place in common age-related macular degeneration eye disease. The purpose of this study was to evaluate whether wounding and wound healing has an effect on Ca2+ dynamics in human embryonic stem cell (hESC)-RPEs cultured different periods of time. Methods: The 9-day-cultured or 28-day-cultured hESC-RPEs from two different cell lines were wounded and the dynamics of spontaneous and mechanically induced intracellular Ca2+ activity was measured with live-cell Ca2+ imaging either immediately or 7days after wounding. The healing time and speed were analyzed with time-lapse bright field microscopy. The Ca2+ activity and healing speed were analysed with image analysis. In addition the extracellular matrix deposition was assessed with confocal microscopy. Results: The Ca2+ dynamics in hESC-RPE monolayers differed depending on the culture time: 9-day-cultured cells had higher number of cells with spontaneous Ca2+ activity close to freshly wounded edge compared to control areas, whereas in 28-day-cultured cells there was no difference in wounded and control areas. The 28-day-cultured, wounded and 7-day-healed hESC-RPEs produced wide-spreading intercellular Ca2+ waves upon mechanical stimulation, while in controls propagation was restricted. Most importantly, both wave spreading and spontaneous Ca2+ activity of cells within the healed area, as well as the cell morphology of 28-day-cultured, wounded and thereafter 7-day-healed areas resembled the 9-day-cultured hESC-RPEs. Conclusions: This acquired knowledge about Ca2+ dynamics of wounded hESC-RPE monolayers is important for understanding the dynamics of RPE wound healing, and could offer a reliable functionality test for RPE cells. The data presented in here suggests that assessment of Ca2+ dynamics analysed with image analysis could be used as a reliable non-invasive functionality test for RPE cells.

AB - Background: Wound healing of retinal pigment epithelium (RPE) is a complex process that may take place in common age-related macular degeneration eye disease. The purpose of this study was to evaluate whether wounding and wound healing has an effect on Ca2+ dynamics in human embryonic stem cell (hESC)-RPEs cultured different periods of time. Methods: The 9-day-cultured or 28-day-cultured hESC-RPEs from two different cell lines were wounded and the dynamics of spontaneous and mechanically induced intracellular Ca2+ activity was measured with live-cell Ca2+ imaging either immediately or 7days after wounding. The healing time and speed were analyzed with time-lapse bright field microscopy. The Ca2+ activity and healing speed were analysed with image analysis. In addition the extracellular matrix deposition was assessed with confocal microscopy. Results: The Ca2+ dynamics in hESC-RPE monolayers differed depending on the culture time: 9-day-cultured cells had higher number of cells with spontaneous Ca2+ activity close to freshly wounded edge compared to control areas, whereas in 28-day-cultured cells there was no difference in wounded and control areas. The 28-day-cultured, wounded and 7-day-healed hESC-RPEs produced wide-spreading intercellular Ca2+ waves upon mechanical stimulation, while in controls propagation was restricted. Most importantly, both wave spreading and spontaneous Ca2+ activity of cells within the healed area, as well as the cell morphology of 28-day-cultured, wounded and thereafter 7-day-healed areas resembled the 9-day-cultured hESC-RPEs. Conclusions: This acquired knowledge about Ca2+ dynamics of wounded hESC-RPE monolayers is important for understanding the dynamics of RPE wound healing, and could offer a reliable functionality test for RPE cells. The data presented in here suggests that assessment of Ca2+ dynamics analysed with image analysis could be used as a reliable non-invasive functionality test for RPE cells.

KW - Ca waves

KW - Cell maturation

KW - HESC-RPE

KW - Image analysis

KW - Mechanical stimulation

KW - Mechanically induced intercellular Ca waves

KW - RPE

KW - Spontaneous [Ca] increases

KW - Wound healing

U2 - 10.1186/s12938-018-0535-z

DO - 10.1186/s12938-018-0535-z

M3 - Article

VL - 17

JO - BioMedical Engineering Online

JF - BioMedical Engineering Online

SN - 1475-925X

IS - 1

M1 - 102

ER -