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X-ray microtomography is a novel method for accurate evaluation of small-bowel mucosal morphology and surface area

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X-ray microtomography is a novel method for accurate evaluation of small-bowel mucosal morphology and surface area. / Virta, Johannes; Hannula, Markus; Tamminen, Ilmari; Lindfors, Katri; Kaukinen, Katri; Popp, Alina; Taavela, Juha; Saavalainen, Päivi; Hiltunen, Pauliina; Hyttinen, Jari; Kurppa, Kalle.

In: Scientific Reports, Vol. 10, No. 1, 13164, 2020.

Research output: Contribution to journalArticleScientificpeer-review

Harvard

Virta, J, Hannula, M, Tamminen, I, Lindfors, K, Kaukinen, K, Popp, A, Taavela, J, Saavalainen, P, Hiltunen, P, Hyttinen, J & Kurppa, K 2020, 'X-ray microtomography is a novel method for accurate evaluation of small-bowel mucosal morphology and surface area', Scientific Reports, vol. 10, no. 1, 13164. https://doi.org/10.1038/s41598-020-69487-w

APA

Virta, J., Hannula, M., Tamminen, I., Lindfors, K., Kaukinen, K., Popp, A., ... Kurppa, K. (2020). X-ray microtomography is a novel method for accurate evaluation of small-bowel mucosal morphology and surface area. Scientific Reports, 10(1), [13164]. https://doi.org/10.1038/s41598-020-69487-w

Vancouver

Virta J, Hannula M, Tamminen I, Lindfors K, Kaukinen K, Popp A et al. X-ray microtomography is a novel method for accurate evaluation of small-bowel mucosal morphology and surface area. Scientific Reports. 2020;10(1). 13164. https://doi.org/10.1038/s41598-020-69487-w

Author

Virta, Johannes ; Hannula, Markus ; Tamminen, Ilmari ; Lindfors, Katri ; Kaukinen, Katri ; Popp, Alina ; Taavela, Juha ; Saavalainen, Päivi ; Hiltunen, Pauliina ; Hyttinen, Jari ; Kurppa, Kalle. / X-ray microtomography is a novel method for accurate evaluation of small-bowel mucosal morphology and surface area. In: Scientific Reports. 2020 ; Vol. 10, No. 1.

Bibtex - Download

@article{296bd11977ed4482b6c38c31cc87ab83,
title = "X-ray microtomography is a novel method for accurate evaluation of small-bowel mucosal morphology and surface area",
abstract = "The often poorly orientated small-bowel mucosal biopsies taken for the diagnostics of celiac disease and other intestinal disorders are prone to misinterpretation. Furthermore, conventional histopathology has suboptimal sensitivity for early histopathological changes observed in short-term challenge studies. X-ray microtomography (micro-CT) is a promising new method for accurate imaging of human-derived biological samples. Here, we report that micro-CT could be utilized to create virtual reconstructions of endoscopically obtained intestinal biopsies. The formed digital 3D images enabled selection of always optimal cutting angles for accurate measurement of the mucosal damage and revealed diagnostic lesions in cases interpreted as normal with conventional histomorphometry. We also demonstrate that computer-assisted point cloud analysis can be used to calculate biologically meaningful surface areas of the biopsies in different stages of mucosal damage with excellent replicability and correlation with other disease parameters. We expect the improved diagnostic accuracy and capability to measure the surface areas to provide a powerful tool for the diagnostics of intestinal diseases and for future clinical and pharmaceutical trials.",
author = "Johannes Virta and Markus Hannula and Ilmari Tamminen and Katri Lindfors and Katri Kaukinen and Alina Popp and Juha Taavela and P{\"a}ivi Saavalainen and Pauliina Hiltunen and Jari Hyttinen and Kalle Kurppa",
year = "2020",
doi = "10.1038/s41598-020-69487-w",
language = "English",
volume = "10",
journal = "Scientific Reports",
issn = "2045-2322",
publisher = "Nature Publishing Group",
number = "1",

}

RIS (suitable for import to EndNote) - Download

TY - JOUR

T1 - X-ray microtomography is a novel method for accurate evaluation of small-bowel mucosal morphology and surface area

AU - Virta, Johannes

AU - Hannula, Markus

AU - Tamminen, Ilmari

AU - Lindfors, Katri

AU - Kaukinen, Katri

AU - Popp, Alina

AU - Taavela, Juha

AU - Saavalainen, Päivi

AU - Hiltunen, Pauliina

AU - Hyttinen, Jari

AU - Kurppa, Kalle

PY - 2020

Y1 - 2020

N2 - The often poorly orientated small-bowel mucosal biopsies taken for the diagnostics of celiac disease and other intestinal disorders are prone to misinterpretation. Furthermore, conventional histopathology has suboptimal sensitivity for early histopathological changes observed in short-term challenge studies. X-ray microtomography (micro-CT) is a promising new method for accurate imaging of human-derived biological samples. Here, we report that micro-CT could be utilized to create virtual reconstructions of endoscopically obtained intestinal biopsies. The formed digital 3D images enabled selection of always optimal cutting angles for accurate measurement of the mucosal damage and revealed diagnostic lesions in cases interpreted as normal with conventional histomorphometry. We also demonstrate that computer-assisted point cloud analysis can be used to calculate biologically meaningful surface areas of the biopsies in different stages of mucosal damage with excellent replicability and correlation with other disease parameters. We expect the improved diagnostic accuracy and capability to measure the surface areas to provide a powerful tool for the diagnostics of intestinal diseases and for future clinical and pharmaceutical trials.

AB - The often poorly orientated small-bowel mucosal biopsies taken for the diagnostics of celiac disease and other intestinal disorders are prone to misinterpretation. Furthermore, conventional histopathology has suboptimal sensitivity for early histopathological changes observed in short-term challenge studies. X-ray microtomography (micro-CT) is a promising new method for accurate imaging of human-derived biological samples. Here, we report that micro-CT could be utilized to create virtual reconstructions of endoscopically obtained intestinal biopsies. The formed digital 3D images enabled selection of always optimal cutting angles for accurate measurement of the mucosal damage and revealed diagnostic lesions in cases interpreted as normal with conventional histomorphometry. We also demonstrate that computer-assisted point cloud analysis can be used to calculate biologically meaningful surface areas of the biopsies in different stages of mucosal damage with excellent replicability and correlation with other disease parameters. We expect the improved diagnostic accuracy and capability to measure the surface areas to provide a powerful tool for the diagnostics of intestinal diseases and for future clinical and pharmaceutical trials.

U2 - 10.1038/s41598-020-69487-w

DO - 10.1038/s41598-020-69487-w

M3 - Article

VL - 10

JO - Scientific Reports

JF - Scientific Reports

SN - 2045-2322

IS - 1

M1 - 13164

ER -