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X-ray microtomography of collagen and polylactide samples in liquids

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X-ray microtomography of collagen and polylactide samples in liquids. / Hannula, M.; Haaparanta, A. M.; Tamminen, I.; Aula, A.; Kellomäki, M.; Hyttinen, J.

XIV Mediterranean Conference on Medical and Biological Engineering and Computing 2016: MEDICON 2016, March 31st–April 2nd 2016, Paphos, Cyprus. Springer Verlag, 2016. p. 420-424 (IFMBE Proceedings; Vol. 57).

Research output: Chapter in Book/Report/Conference proceedingConference contributionScientificpeer-review

Harvard

Hannula, M, Haaparanta, AM, Tamminen, I, Aula, A, Kellomäki, M & Hyttinen, J 2016, X-ray microtomography of collagen and polylactide samples in liquids. in XIV Mediterranean Conference on Medical and Biological Engineering and Computing 2016: MEDICON 2016, March 31st–April 2nd 2016, Paphos, Cyprus. IFMBE Proceedings, vol. 57, Springer Verlag, pp. 420-424, Mediterranean Conference on Medical and Biological Engineering and Computing, 1/01/00. https://doi.org/10.1007/978-3-319-32703-7_82

APA

Hannula, M., Haaparanta, A. M., Tamminen, I., Aula, A., Kellomäki, M., & Hyttinen, J. (2016). X-ray microtomography of collagen and polylactide samples in liquids. In XIV Mediterranean Conference on Medical and Biological Engineering and Computing 2016: MEDICON 2016, March 31st–April 2nd 2016, Paphos, Cyprus (pp. 420-424). (IFMBE Proceedings; Vol. 57). Springer Verlag. https://doi.org/10.1007/978-3-319-32703-7_82

Vancouver

Hannula M, Haaparanta AM, Tamminen I, Aula A, Kellomäki M, Hyttinen J. X-ray microtomography of collagen and polylactide samples in liquids. In XIV Mediterranean Conference on Medical and Biological Engineering and Computing 2016: MEDICON 2016, March 31st–April 2nd 2016, Paphos, Cyprus. Springer Verlag. 2016. p. 420-424. (IFMBE Proceedings). https://doi.org/10.1007/978-3-319-32703-7_82

Author

Hannula, M. ; Haaparanta, A. M. ; Tamminen, I. ; Aula, A. ; Kellomäki, M. ; Hyttinen, J. / X-ray microtomography of collagen and polylactide samples in liquids. XIV Mediterranean Conference on Medical and Biological Engineering and Computing 2016: MEDICON 2016, March 31st–April 2nd 2016, Paphos, Cyprus. Springer Verlag, 2016. pp. 420-424 (IFMBE Proceedings).

Bibtex - Download

@inproceedings{6a93c6d919564a278b0af4213dce1839,
title = "X-ray microtomography of collagen and polylactide samples in liquids",
abstract = "Methods to image and assess the microstructure of polymer based biomaterials in liquid phase, for example cell culture medium, are well warranted. X-ray microtomography could provide a mean to visualize and analyze such structures. However, the density of such polymers is close to that of water and hence the inherent X-ray contrast is poor. The material can provide good contrast when dry, however, if the materials contain cells and are dried, the cell morphology may be distorted. Moreover the entire structure of these water containing materials are deformed in the drying process. In this paper we tested phosphotungstic acid (PTA) staining to improve the contrast. We imaged collagen and PLA samples, as well as collagen-PLA composites with μCT in air, water and alcohol. The methods were compared visually and with contrast to noise ratio calculated from the images. Our results demonstrate that with alcohol the PLA can be imaged also in liquid phase. PTA staining seems to be a good method to increase the contrast for collagen in μCT imaging.",
keywords = "Collagen, Polylactide, Staining, μCT imaging",
author = "M. Hannula and Haaparanta, {A. M.} and I. Tamminen and A. Aula and M. Kellom{\"a}ki and J. Hyttinen",
note = "JUFOID=58152",
year = "2016",
doi = "10.1007/978-3-319-32703-7_82",
language = "English",
isbn = "9783319327013",
series = "IFMBE Proceedings",
publisher = "Springer Verlag",
pages = "420--424",
booktitle = "XIV Mediterranean Conference on Medical and Biological Engineering and Computing 2016",
address = "Germany",

}

RIS (suitable for import to EndNote) - Download

TY - GEN

T1 - X-ray microtomography of collagen and polylactide samples in liquids

AU - Hannula, M.

AU - Haaparanta, A. M.

AU - Tamminen, I.

AU - Aula, A.

AU - Kellomäki, M.

AU - Hyttinen, J.

N1 - JUFOID=58152

PY - 2016

Y1 - 2016

N2 - Methods to image and assess the microstructure of polymer based biomaterials in liquid phase, for example cell culture medium, are well warranted. X-ray microtomography could provide a mean to visualize and analyze such structures. However, the density of such polymers is close to that of water and hence the inherent X-ray contrast is poor. The material can provide good contrast when dry, however, if the materials contain cells and are dried, the cell morphology may be distorted. Moreover the entire structure of these water containing materials are deformed in the drying process. In this paper we tested phosphotungstic acid (PTA) staining to improve the contrast. We imaged collagen and PLA samples, as well as collagen-PLA composites with μCT in air, water and alcohol. The methods were compared visually and with contrast to noise ratio calculated from the images. Our results demonstrate that with alcohol the PLA can be imaged also in liquid phase. PTA staining seems to be a good method to increase the contrast for collagen in μCT imaging.

AB - Methods to image and assess the microstructure of polymer based biomaterials in liquid phase, for example cell culture medium, are well warranted. X-ray microtomography could provide a mean to visualize and analyze such structures. However, the density of such polymers is close to that of water and hence the inherent X-ray contrast is poor. The material can provide good contrast when dry, however, if the materials contain cells and are dried, the cell morphology may be distorted. Moreover the entire structure of these water containing materials are deformed in the drying process. In this paper we tested phosphotungstic acid (PTA) staining to improve the contrast. We imaged collagen and PLA samples, as well as collagen-PLA composites with μCT in air, water and alcohol. The methods were compared visually and with contrast to noise ratio calculated from the images. Our results demonstrate that with alcohol the PLA can be imaged also in liquid phase. PTA staining seems to be a good method to increase the contrast for collagen in μCT imaging.

KW - Collagen

KW - Polylactide

KW - Staining

KW - μCT imaging

U2 - 10.1007/978-3-319-32703-7_82

DO - 10.1007/978-3-319-32703-7_82

M3 - Conference contribution

SN - 9783319327013

T3 - IFMBE Proceedings

SP - 420

EP - 424

BT - XIV Mediterranean Conference on Medical and Biological Engineering and Computing 2016

PB - Springer Verlag

ER -