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A Comparative View on Easy to Deploy non-Integrating Methods for Patient-Specific iPSC Production

Tutkimustuotosvertaisarvioitu

Standard

A Comparative View on Easy to Deploy non-Integrating Methods for Patient-Specific iPSC Production. / Manzini, Stefano; Viiri, Leena E.; Marttila, Suvi; Aalto-Setälä, Katriina.

julkaisussa: Stem Cell Reviews and Reports, Vuosikerta 11, Nro 6, 01.12.2015, s. 900-908.

Tutkimustuotosvertaisarvioitu

Harvard

Manzini, S, Viiri, LE, Marttila, S & Aalto-Setälä, K 2015, 'A Comparative View on Easy to Deploy non-Integrating Methods for Patient-Specific iPSC Production', Stem Cell Reviews and Reports, Vuosikerta. 11, Nro 6, Sivut 900-908. https://doi.org/10.1007/s12015-015-9619-3

APA

Manzini, S., Viiri, L. E., Marttila, S., & Aalto-Setälä, K. (2015). A Comparative View on Easy to Deploy non-Integrating Methods for Patient-Specific iPSC Production. Stem Cell Reviews and Reports, 11(6), 900-908. https://doi.org/10.1007/s12015-015-9619-3

Vancouver

Manzini S, Viiri LE, Marttila S, Aalto-Setälä K. A Comparative View on Easy to Deploy non-Integrating Methods for Patient-Specific iPSC Production. Stem Cell Reviews and Reports. 2015 joulu 1;11(6):900-908. https://doi.org/10.1007/s12015-015-9619-3

Author

Manzini, Stefano ; Viiri, Leena E. ; Marttila, Suvi ; Aalto-Setälä, Katriina. / A Comparative View on Easy to Deploy non-Integrating Methods for Patient-Specific iPSC Production. Julkaisussa: Stem Cell Reviews and Reports. 2015 ; Vuosikerta 11, Nro 6. Sivut 900-908.

Bibtex - Lataa

@article{f3bcc1f56f66439098d1f9a7e9ae9502,
title = "A Comparative View on Easy to Deploy non-Integrating Methods for Patient-Specific iPSC Production",
abstract = "Induced pluripotent stem cells (iPSCs) are routinely produced from dermal fibroblasts, with potential applications ranging from in vitro disease models to drug discovery and regenerative medicine. The need of eliminating the remaining reprogramming factors after iPSC production spurred the development of non-integrating viruses such as Sendai and other methods to deliver episomal vectors, which are progressively lost upon cell division. We compared four widespread methods (Sendai virus, Nucleofector, Neon transfection system and Lipofectamine 3000) to generate integration-free iPSC lines from primary human dermal fibroblasts (hDF) of three patients. Furthermore, we performed extensive characterization of the iPSC lines. We were able to produce iPSC lines with all tested methods with variable efficiency. Sendai virus method achieved the overall highest reprogramming rate, followed by electroporation-based methods Nucleofector and Neon transfection systems. Chemical-based Lipofectamine 3000 delivery resulted in the lowest number of iPSC colonies. We found the reprogramming rate to be intrinsically dependent on the individual hDFs but the amenability of each hDF to reprogramming showed consistency between methods. Regardless of the reprogramming strategy, iPSCs obtained did not reveal any significant differences in their morphology, expression of pluripotency markers, EB formation, karyotype or gene expression profiles.",
keywords = "Electroporation, Episomal, Human dermal fibroblast, Induced pluripotent stem cells (iPSC), Plasmid, Reprogramming",
author = "Stefano Manzini and Viiri, {Leena E.} and Suvi Marttila and Katriina Aalto-Set{\"a}l{\"a}",
year = "2015",
month = "12",
day = "1",
doi = "10.1007/s12015-015-9619-3",
language = "English",
volume = "11",
pages = "900--908",
journal = "Stem Cell Reviews and Reports",
issn = "1550-8943",
publisher = "Springer Verlag",
number = "6",

}

RIS (suitable for import to EndNote) - Lataa

TY - JOUR

T1 - A Comparative View on Easy to Deploy non-Integrating Methods for Patient-Specific iPSC Production

AU - Manzini, Stefano

AU - Viiri, Leena E.

AU - Marttila, Suvi

AU - Aalto-Setälä, Katriina

PY - 2015/12/1

Y1 - 2015/12/1

N2 - Induced pluripotent stem cells (iPSCs) are routinely produced from dermal fibroblasts, with potential applications ranging from in vitro disease models to drug discovery and regenerative medicine. The need of eliminating the remaining reprogramming factors after iPSC production spurred the development of non-integrating viruses such as Sendai and other methods to deliver episomal vectors, which are progressively lost upon cell division. We compared four widespread methods (Sendai virus, Nucleofector, Neon transfection system and Lipofectamine 3000) to generate integration-free iPSC lines from primary human dermal fibroblasts (hDF) of three patients. Furthermore, we performed extensive characterization of the iPSC lines. We were able to produce iPSC lines with all tested methods with variable efficiency. Sendai virus method achieved the overall highest reprogramming rate, followed by electroporation-based methods Nucleofector and Neon transfection systems. Chemical-based Lipofectamine 3000 delivery resulted in the lowest number of iPSC colonies. We found the reprogramming rate to be intrinsically dependent on the individual hDFs but the amenability of each hDF to reprogramming showed consistency between methods. Regardless of the reprogramming strategy, iPSCs obtained did not reveal any significant differences in their morphology, expression of pluripotency markers, EB formation, karyotype or gene expression profiles.

AB - Induced pluripotent stem cells (iPSCs) are routinely produced from dermal fibroblasts, with potential applications ranging from in vitro disease models to drug discovery and regenerative medicine. The need of eliminating the remaining reprogramming factors after iPSC production spurred the development of non-integrating viruses such as Sendai and other methods to deliver episomal vectors, which are progressively lost upon cell division. We compared four widespread methods (Sendai virus, Nucleofector, Neon transfection system and Lipofectamine 3000) to generate integration-free iPSC lines from primary human dermal fibroblasts (hDF) of three patients. Furthermore, we performed extensive characterization of the iPSC lines. We were able to produce iPSC lines with all tested methods with variable efficiency. Sendai virus method achieved the overall highest reprogramming rate, followed by electroporation-based methods Nucleofector and Neon transfection systems. Chemical-based Lipofectamine 3000 delivery resulted in the lowest number of iPSC colonies. We found the reprogramming rate to be intrinsically dependent on the individual hDFs but the amenability of each hDF to reprogramming showed consistency between methods. Regardless of the reprogramming strategy, iPSCs obtained did not reveal any significant differences in their morphology, expression of pluripotency markers, EB formation, karyotype or gene expression profiles.

KW - Electroporation

KW - Episomal

KW - Human dermal fibroblast

KW - Induced pluripotent stem cells (iPSC)

KW - Plasmid

KW - Reprogramming

UR - http://www.scopus.com/inward/record.url?scp=84947616670&partnerID=8YFLogxK

U2 - 10.1007/s12015-015-9619-3

DO - 10.1007/s12015-015-9619-3

M3 - Article

VL - 11

SP - 900

EP - 908

JO - Stem Cell Reviews and Reports

JF - Stem Cell Reviews and Reports

SN - 1550-8943

IS - 6

ER -