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A comparison of immunogenicity of norovirus GII-4 virus-like particles and P-particles

Tutkimustuotosvertaisarvioitu

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A comparison of immunogenicity of norovirus GII-4 virus-like particles and P-particles. / Tamminen, Kirsi; Huhti, Leena; Koho, Tiia; Lappalainen, Suvi; Hytönen, Vesa P.; Vesikari, Timo; Blazevic, Vesna.

julkaisussa: IMMUNOLOGY, Vuosikerta 135, Nro 1, 01.2012, s. 89-99.

Tutkimustuotosvertaisarvioitu

Harvard

Tamminen, K, Huhti, L, Koho, T, Lappalainen, S, Hytönen, VP, Vesikari, T & Blazevic, V 2012, 'A comparison of immunogenicity of norovirus GII-4 virus-like particles and P-particles', IMMUNOLOGY, Vuosikerta. 135, Nro 1, Sivut 89-99. https://doi.org/10.1111/j.1365-2567.2011.03516.x

APA

Tamminen, K., Huhti, L., Koho, T., Lappalainen, S., Hytönen, V. P., Vesikari, T., & Blazevic, V. (2012). A comparison of immunogenicity of norovirus GII-4 virus-like particles and P-particles. IMMUNOLOGY, 135(1), 89-99. https://doi.org/10.1111/j.1365-2567.2011.03516.x

Vancouver

Tamminen K, Huhti L, Koho T, Lappalainen S, Hytönen VP, Vesikari T et al. A comparison of immunogenicity of norovirus GII-4 virus-like particles and P-particles. IMMUNOLOGY. 2012 tammi;135(1):89-99. https://doi.org/10.1111/j.1365-2567.2011.03516.x

Author

Tamminen, Kirsi ; Huhti, Leena ; Koho, Tiia ; Lappalainen, Suvi ; Hytönen, Vesa P. ; Vesikari, Timo ; Blazevic, Vesna. / A comparison of immunogenicity of norovirus GII-4 virus-like particles and P-particles. Julkaisussa: IMMUNOLOGY. 2012 ; Vuosikerta 135, Nro 1. Sivut 89-99.

Bibtex - Lataa

@article{74f71565e50442ddb36e1057ec76ebb2,
title = "A comparison of immunogenicity of norovirus GII-4 virus-like particles and P-particles",
abstract = "Norovirus (NoV) -derived virus-like particles (VLPs) resemble empty shells of the virus and NoV P-particles contain only protruding domains of the NoV capsid. Both NoV-derived subviral particles show similar functionality and antigenicity in vitro and are considered to be potential vaccine candidates against NoV gastroenteritis. BALB/c mice were immunized with baculovirus-produced GII-4 VLPs or the corresponding Escherichia coli-produced P-particles by the intramuscular or intradermal route and the NoV-specific antibody and T-cell immune responses were compared. Elevated antibody levels were induced with a single VLP immunization, whereas P-particle immunization required a boost. High avidity antibodies were raised only by VLP immunization. VLP immunization resulted in a balanced T helper type 1/type 2 immune response whereas P-particles induced a T helper type 2-biased response. Only VLP immunization primed T cells for interferon-γ production. Most importantly, cross-reactive B and T cells were induced solely by VLP immunization. In addition, VLP antiserum blocked the binding of heterotypic VLPs to human histo-blood group antigen receptor and saliva. The findings in this study are relevant for the development of NoV vaccines.",
keywords = "Histo-blood group antigen blocking, Immune response, Norovirus, P-particles, Virus-like particles",
author = "Kirsi Tamminen and Leena Huhti and Tiia Koho and Suvi Lappalainen and Hyt{\"o}nen, {Vesa P.} and Timo Vesikari and Vesna Blazevic",
year = "2012",
month = "1",
doi = "10.1111/j.1365-2567.2011.03516.x",
language = "English",
volume = "135",
pages = "89--99",
journal = "IMMUNOLOGY",
issn = "0019-2805",
publisher = "Wiley",
number = "1",

}

RIS (suitable for import to EndNote) - Lataa

TY - JOUR

T1 - A comparison of immunogenicity of norovirus GII-4 virus-like particles and P-particles

AU - Tamminen, Kirsi

AU - Huhti, Leena

AU - Koho, Tiia

AU - Lappalainen, Suvi

AU - Hytönen, Vesa P.

AU - Vesikari, Timo

AU - Blazevic, Vesna

PY - 2012/1

Y1 - 2012/1

N2 - Norovirus (NoV) -derived virus-like particles (VLPs) resemble empty shells of the virus and NoV P-particles contain only protruding domains of the NoV capsid. Both NoV-derived subviral particles show similar functionality and antigenicity in vitro and are considered to be potential vaccine candidates against NoV gastroenteritis. BALB/c mice were immunized with baculovirus-produced GII-4 VLPs or the corresponding Escherichia coli-produced P-particles by the intramuscular or intradermal route and the NoV-specific antibody and T-cell immune responses were compared. Elevated antibody levels were induced with a single VLP immunization, whereas P-particle immunization required a boost. High avidity antibodies were raised only by VLP immunization. VLP immunization resulted in a balanced T helper type 1/type 2 immune response whereas P-particles induced a T helper type 2-biased response. Only VLP immunization primed T cells for interferon-γ production. Most importantly, cross-reactive B and T cells were induced solely by VLP immunization. In addition, VLP antiserum blocked the binding of heterotypic VLPs to human histo-blood group antigen receptor and saliva. The findings in this study are relevant for the development of NoV vaccines.

AB - Norovirus (NoV) -derived virus-like particles (VLPs) resemble empty shells of the virus and NoV P-particles contain only protruding domains of the NoV capsid. Both NoV-derived subviral particles show similar functionality and antigenicity in vitro and are considered to be potential vaccine candidates against NoV gastroenteritis. BALB/c mice were immunized with baculovirus-produced GII-4 VLPs or the corresponding Escherichia coli-produced P-particles by the intramuscular or intradermal route and the NoV-specific antibody and T-cell immune responses were compared. Elevated antibody levels were induced with a single VLP immunization, whereas P-particle immunization required a boost. High avidity antibodies were raised only by VLP immunization. VLP immunization resulted in a balanced T helper type 1/type 2 immune response whereas P-particles induced a T helper type 2-biased response. Only VLP immunization primed T cells for interferon-γ production. Most importantly, cross-reactive B and T cells were induced solely by VLP immunization. In addition, VLP antiserum blocked the binding of heterotypic VLPs to human histo-blood group antigen receptor and saliva. The findings in this study are relevant for the development of NoV vaccines.

KW - Histo-blood group antigen blocking

KW - Immune response

KW - Norovirus

KW - P-particles

KW - Virus-like particles

UR - http://www.scopus.com/inward/record.url?scp=83055188637&partnerID=8YFLogxK

U2 - 10.1111/j.1365-2567.2011.03516.x

DO - 10.1111/j.1365-2567.2011.03516.x

M3 - Article

VL - 135

SP - 89

EP - 99

JO - IMMUNOLOGY

JF - IMMUNOLOGY

SN - 0019-2805

IS - 1

ER -