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A mathematical model and iterative inversion for fluorescent optical projection tomography

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A mathematical model and iterative inversion for fluorescent optical projection tomography. / Koljonen, Ville; Koskela, Olli; Montonen, Toni; Rezaei, Atena; Belay, Birhanu; Figueiras, Edite; Hyttinen, Jari; Pursiainen, Sampsa.

julkaisussa: Physics in Medicine and Biology, Vuosikerta 64, Nro 4, 045017, 18.02.2019.

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Koljonen V, Koskela O, Montonen T, Rezaei A, Belay B, Figueiras E et al. A mathematical model and iterative inversion for fluorescent optical projection tomography. Physics in Medicine and Biology. 2019 helmi 18;64(4). 045017. https://doi.org/10.1088/1361-6560/aafd63

Author

Koljonen, Ville ; Koskela, Olli ; Montonen, Toni ; Rezaei, Atena ; Belay, Birhanu ; Figueiras, Edite ; Hyttinen, Jari ; Pursiainen, Sampsa. / A mathematical model and iterative inversion for fluorescent optical projection tomography. Julkaisussa: Physics in Medicine and Biology. 2019 ; Vuosikerta 64, Nro 4.

Bibtex - Lataa

@article{7c457881f90d418ba2243be3b794ff7c,
title = "A mathematical model and iterative inversion for fluorescent optical projection tomography",
abstract = "Solving the fluorophore distribution in a tomographic setting has been difficult because of the lack of physically meaningful and computationally applicable propagation models. This study concentrates on the direct modelling of fluorescence signals in optical projection tomography (OPT), and on the corresponding inverse problem. The reconstruction problem is solved using emission projections corresponding to a series of rotational imaging positions of the sample. Similarly to the bright field OPT bearing resemblance with the transmission x-ray computed tomography, the fluorescent mode OPT is analogous to x-ray fluorescence tomography (XFCT). As an improved direct model for the fluorescent OPT, we derive a weighted Radon transform based on the XFCT literature. Moreover, we propose a simple and fast iteration scheme for the slice-wise reconstruction of the sample. The developed methods are applied in both numerical experiments and inversion of fluorescent OPT data from a zebrafish embryo. The results demonstrate the importance of propagation modelling and our analysis provides a flexible modelling framework for fluorescent OPT that can easily be modified to adapt to different imaging setups.",
keywords = "beam modelling, fluorescence tomography, iterative reconstruction, optical projection tomography, weighted Radon transform",
author = "Ville Koljonen and Olli Koskela and Toni Montonen and Atena Rezaei and Birhanu Belay and Edite Figueiras and Jari Hyttinen and Sampsa Pursiainen",
note = "DUPL=48034291",
year = "2019",
month = "2",
day = "18",
doi = "10.1088/1361-6560/aafd63",
language = "English",
volume = "64",
journal = "Physics in Medicine and Biology",
issn = "0031-9155",
publisher = "IOP Publishing Ltd.",
number = "4",

}

RIS (suitable for import to EndNote) - Lataa

TY - JOUR

T1 - A mathematical model and iterative inversion for fluorescent optical projection tomography

AU - Koljonen, Ville

AU - Koskela, Olli

AU - Montonen, Toni

AU - Rezaei, Atena

AU - Belay, Birhanu

AU - Figueiras, Edite

AU - Hyttinen, Jari

AU - Pursiainen, Sampsa

N1 - DUPL=48034291

PY - 2019/2/18

Y1 - 2019/2/18

N2 - Solving the fluorophore distribution in a tomographic setting has been difficult because of the lack of physically meaningful and computationally applicable propagation models. This study concentrates on the direct modelling of fluorescence signals in optical projection tomography (OPT), and on the corresponding inverse problem. The reconstruction problem is solved using emission projections corresponding to a series of rotational imaging positions of the sample. Similarly to the bright field OPT bearing resemblance with the transmission x-ray computed tomography, the fluorescent mode OPT is analogous to x-ray fluorescence tomography (XFCT). As an improved direct model for the fluorescent OPT, we derive a weighted Radon transform based on the XFCT literature. Moreover, we propose a simple and fast iteration scheme for the slice-wise reconstruction of the sample. The developed methods are applied in both numerical experiments and inversion of fluorescent OPT data from a zebrafish embryo. The results demonstrate the importance of propagation modelling and our analysis provides a flexible modelling framework for fluorescent OPT that can easily be modified to adapt to different imaging setups.

AB - Solving the fluorophore distribution in a tomographic setting has been difficult because of the lack of physically meaningful and computationally applicable propagation models. This study concentrates on the direct modelling of fluorescence signals in optical projection tomography (OPT), and on the corresponding inverse problem. The reconstruction problem is solved using emission projections corresponding to a series of rotational imaging positions of the sample. Similarly to the bright field OPT bearing resemblance with the transmission x-ray computed tomography, the fluorescent mode OPT is analogous to x-ray fluorescence tomography (XFCT). As an improved direct model for the fluorescent OPT, we derive a weighted Radon transform based on the XFCT literature. Moreover, we propose a simple and fast iteration scheme for the slice-wise reconstruction of the sample. The developed methods are applied in both numerical experiments and inversion of fluorescent OPT data from a zebrafish embryo. The results demonstrate the importance of propagation modelling and our analysis provides a flexible modelling framework for fluorescent OPT that can easily be modified to adapt to different imaging setups.

KW - beam modelling

KW - fluorescence tomography

KW - iterative reconstruction

KW - optical projection tomography

KW - weighted Radon transform

U2 - 10.1088/1361-6560/aafd63

DO - 10.1088/1361-6560/aafd63

M3 - Article

VL - 64

JO - Physics in Medicine and Biology

JF - Physics in Medicine and Biology

SN - 0031-9155

IS - 4

M1 - 045017

ER -