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An in vitro study of composites of poly(L-lactide-co-e-caprolactone), ß-tricalcium phosphate and ciprofloxacin intended for local treatment of osteomyelitis

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An in vitro study of composites of poly(L-lactide-co-e-caprolactone), ß-tricalcium phosphate and ciprofloxacin intended for local treatment of osteomyelitis. / Ahola, Niina; Männistö, Noora; Veiranto, Minna; Karp, Matti; Rich, Jaana; Efimov, Alexander; Seppälä, Jukka; Kellomäki, Minna.

julkaisussa: Biomatter, Vuosikerta 3, Nro 2, e23162, 2013, s. 1-13.

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Ahola, Niina ; Männistö, Noora ; Veiranto, Minna ; Karp, Matti ; Rich, Jaana ; Efimov, Alexander ; Seppälä, Jukka ; Kellomäki, Minna. / An in vitro study of composites of poly(L-lactide-co-e-caprolactone), ß-tricalcium phosphate and ciprofloxacin intended for local treatment of osteomyelitis. Julkaisussa: Biomatter. 2013 ; Vuosikerta 3, Nro 2. Sivut 1-13.

Bibtex - Lataa

@article{17d8cd46ad8d4b60aa415f41ee069b98,
title = "An in vitro study of composites of poly(L-lactide-co-e-caprolactone), {\ss}-tricalcium phosphate and ciprofloxacin intended for local treatment of osteomyelitis",
abstract = "Osteomyelitis is a bacterial disease that can become chronic, and treatment often includes a surgical operation to remove infected bone. The aim of this study was to develop and investigate in vitro bone filling composite materials that release ciprofloxacin to kill any remaining bacteria and contain bioceramic to help the bone to heal. Three composites of poly(Llactide-co-ε-caprolactone), β-tricalcium phosphate and ciprofloxacin were compounded using twin-screw extrusion and sterilized by gamma irradiation. Drug release and degradation of the composites were investigated in vitro for 52 weeks. The composite with 50 wt{\%} of β-TCP had the most promising ciprofloxacin release profile. The ceramic component accelerated the drug release that occurred in three phases obeying first-order kinetics. Inhibition zone testing using bioluminescence showed that the released ciprofloxacin had effect in eradicating a common osteomyelitis causing bacteria Pseudomonas aeruginosa. During the in vitro degradation test series, molar weight of the polymer matrix of the composites decreased rapidly. Additionally, 1H-NMR analysis showed that the polymer had blocky structure and the comonomer ratio changed during hydrolysis. The tested composites showed great potential to be developed into bone filler materials for the treatment of osteomyelitis or other bone related infections.",
author = "Niina Ahola and Noora M{\"a}nnist{\"o} and Minna Veiranto and Matti Karp and Jaana Rich and Alexander Efimov and Jukka Sepp{\"a}l{\"a} and Minna Kellom{\"a}ki",
note = "Contribution: organisation=elt,FACT1=0.7<br/>Contribution: organisation=keb,FACT2=0.3<br/>Portfolio EDEND: 2013-07-29<br/>Publisher name: Landes Bioscience",
year = "2013",
doi = "10.4161/biom.23162",
language = "English",
volume = "3",
pages = "1--13",
journal = "Biomatter",
issn = "2159-2527",
publisher = "Taylor & Francis",
number = "2",

}

RIS (suitable for import to EndNote) - Lataa

TY - JOUR

T1 - An in vitro study of composites of poly(L-lactide-co-e-caprolactone), ß-tricalcium phosphate and ciprofloxacin intended for local treatment of osteomyelitis

AU - Ahola, Niina

AU - Männistö, Noora

AU - Veiranto, Minna

AU - Karp, Matti

AU - Rich, Jaana

AU - Efimov, Alexander

AU - Seppälä, Jukka

AU - Kellomäki, Minna

N1 - Contribution: organisation=elt,FACT1=0.7<br/>Contribution: organisation=keb,FACT2=0.3<br/>Portfolio EDEND: 2013-07-29<br/>Publisher name: Landes Bioscience

PY - 2013

Y1 - 2013

N2 - Osteomyelitis is a bacterial disease that can become chronic, and treatment often includes a surgical operation to remove infected bone. The aim of this study was to develop and investigate in vitro bone filling composite materials that release ciprofloxacin to kill any remaining bacteria and contain bioceramic to help the bone to heal. Three composites of poly(Llactide-co-ε-caprolactone), β-tricalcium phosphate and ciprofloxacin were compounded using twin-screw extrusion and sterilized by gamma irradiation. Drug release and degradation of the composites were investigated in vitro for 52 weeks. The composite with 50 wt% of β-TCP had the most promising ciprofloxacin release profile. The ceramic component accelerated the drug release that occurred in three phases obeying first-order kinetics. Inhibition zone testing using bioluminescence showed that the released ciprofloxacin had effect in eradicating a common osteomyelitis causing bacteria Pseudomonas aeruginosa. During the in vitro degradation test series, molar weight of the polymer matrix of the composites decreased rapidly. Additionally, 1H-NMR analysis showed that the polymer had blocky structure and the comonomer ratio changed during hydrolysis. The tested composites showed great potential to be developed into bone filler materials for the treatment of osteomyelitis or other bone related infections.

AB - Osteomyelitis is a bacterial disease that can become chronic, and treatment often includes a surgical operation to remove infected bone. The aim of this study was to develop and investigate in vitro bone filling composite materials that release ciprofloxacin to kill any remaining bacteria and contain bioceramic to help the bone to heal. Three composites of poly(Llactide-co-ε-caprolactone), β-tricalcium phosphate and ciprofloxacin were compounded using twin-screw extrusion and sterilized by gamma irradiation. Drug release and degradation of the composites were investigated in vitro for 52 weeks. The composite with 50 wt% of β-TCP had the most promising ciprofloxacin release profile. The ceramic component accelerated the drug release that occurred in three phases obeying first-order kinetics. Inhibition zone testing using bioluminescence showed that the released ciprofloxacin had effect in eradicating a common osteomyelitis causing bacteria Pseudomonas aeruginosa. During the in vitro degradation test series, molar weight of the polymer matrix of the composites decreased rapidly. Additionally, 1H-NMR analysis showed that the polymer had blocky structure and the comonomer ratio changed during hydrolysis. The tested composites showed great potential to be developed into bone filler materials for the treatment of osteomyelitis or other bone related infections.

U2 - 10.4161/biom.23162

DO - 10.4161/biom.23162

M3 - Article

VL - 3

SP - 1

EP - 13

JO - Biomatter

JF - Biomatter

SN - 2159-2527

IS - 2

M1 - e23162

ER -