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Analysis of ATP-Induced Ca2+ Responses at Single Cell Level in Retinal Pigment Epithelium Monolayers.

Tutkimustuotosvertaisarvioitu

Yksityiskohdat

AlkuperäiskieliEnglanti
OtsikkoRetinal Degenerative Diseases
AlaotsikkoMechanisms and Experimental Therapy
KustantajaSpringer
Sivut525-530
Sivumäärä6
ISBN (elektroninen)978-3-030-27378-1
ISBN (painettu)978-3-030-27377-4
DOI - pysyväislinkit
TilaJulkaistu - 29 joulukuuta 2019
OKM-julkaisutyyppiA4 Artikkeli konferenssijulkaisussa
TapahtumaInternational Symposium on Retinal Degeneration - Killarney, Irlanti
Kesto: 3 syyskuuta 20188 syyskuuta 2018
Konferenssinumero: XVIII

Julkaisusarja

NimiAdvances in Experimental Medicine and Biology
Vuosikerta1185
ISSN (painettu)0065-2598
ISSN (elektroninen)2214-8019

Conference

ConferenceInternational Symposium on Retinal Degeneration
LyhennettäRD2018
MaaIrlanti
KaupunkiKillarney
Ajanjakso3/09/188/09/18

Tiivistelmä

Calcium is one of the most important second messengers in cells and thus involved in a variety of physiological processes. In retinal pigment epithelium (RPE), Ca2+ and its ATP-dependent signaling pathways play important roles in the retina maintenance functions. Changes in intracellular Ca2+ concentration can be measured from living cells by Ca2+ imaging. Combining these measurements with quantitative analysis of Ca2+ response properties enables studies of signaling pathways affecting RPE functions. However, robust tools for response analysis from large cell populations are lacking. We developed MATLAB-based analysis tools for single cell level Ca2+ response data recorded from large fields of intact RPE monolayers. The analysis revealed significant heterogeneity in ATP-induced Ca2+ responses inside cell populations regarding magnitude and response kinetics. Further analysis including response grouping and parameter correlations allowed us to characterize the populations at the level of single cells.

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