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Analysis of free, mono- and diacetylated polyamines from human urine by LC-MS/MS

Tutkimustuotosvertaisarvioitu

Standard

Analysis of free, mono- and diacetylated polyamines from human urine by LC-MS/MS. / Häkkinen, Merja R.; Roine, Antti; Auriola, Seppo; Tuokko, Antti; Veskimäe, Erik; Keinänen, Tuomo A.; Lehtimäki, Terho; Oksala, Niku; Vepsäläinen, Jouko.

julkaisussa: JOURNAL OF CHROMATOGRAPHY B: ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, Vuosikerta 941, 15.12.2013, s. 81-89.

Tutkimustuotosvertaisarvioitu

Harvard

Häkkinen, MR, Roine, A, Auriola, S, Tuokko, A, Veskimäe, E, Keinänen, TA, Lehtimäki, T, Oksala, N & Vepsäläinen, J 2013, 'Analysis of free, mono- and diacetylated polyamines from human urine by LC-MS/MS', JOURNAL OF CHROMATOGRAPHY B: ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, Vuosikerta. 941, Sivut 81-89. https://doi.org/10.1016/j.jchromb.2013.10.009

APA

Häkkinen, M. R., Roine, A., Auriola, S., Tuokko, A., Veskimäe, E., Keinänen, T. A., ... Vepsäläinen, J. (2013). Analysis of free, mono- and diacetylated polyamines from human urine by LC-MS/MS. JOURNAL OF CHROMATOGRAPHY B: ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 941, 81-89. https://doi.org/10.1016/j.jchromb.2013.10.009

Vancouver

Häkkinen MR, Roine A, Auriola S, Tuokko A, Veskimäe E, Keinänen TA et al. Analysis of free, mono- and diacetylated polyamines from human urine by LC-MS/MS. JOURNAL OF CHROMATOGRAPHY B: ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES. 2013 joulu 15;941:81-89. https://doi.org/10.1016/j.jchromb.2013.10.009

Author

Häkkinen, Merja R. ; Roine, Antti ; Auriola, Seppo ; Tuokko, Antti ; Veskimäe, Erik ; Keinänen, Tuomo A. ; Lehtimäki, Terho ; Oksala, Niku ; Vepsäläinen, Jouko. / Analysis of free, mono- and diacetylated polyamines from human urine by LC-MS/MS. Julkaisussa: JOURNAL OF CHROMATOGRAPHY B: ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES. 2013 ; Vuosikerta 941. Sivut 81-89.

Bibtex - Lataa

@article{b62ab9758c304578871b20c0905df4df,
title = "Analysis of free, mono- and diacetylated polyamines from human urine by LC-MS/MS",
abstract = "Polyamines are promising biochemical markers of cancer and many other pathophysiological conditions, and thus their concentrations in biological fluids are a matter of interest. However, since the concentrations of these compounds are low, their quantitation is typically based on methods requiring laborious sample preparation. Here we developed and validated an LC-MS/MS method to analyze simultaneously free (DAP, PUT, CAD, SPD, SPM) monoacetylated (AcPUT, AcCAD, N1AcSPD, N8AcSPD, N1AcSPM) and diacetylated (DiAcPUT, DiAcCAD, DiAcSPD, DiAcSPM) polyamines from human urine without the need for derivatization. Deuterium labeled polyamines were the internal standards for each analyte. Diluted urine samples spiked with internal standards were filtered through a strong anion exchange resin prior to LC-MS/MS analysis. The chromatographic separation of 14 polyamines was achieved in 12min on C18 column with 0.1{\%} HFBA (v/v) as the ion-pairing agent and a water-acetonitrile gradient. Ionization was performed with positive electrospray ionization (ESI) and detection was with a triple quadrupole mass spectrometer with selected reaction monitoring. Calibration curves ranged from up to 5 to 10,000nM. The accuracy and precision of the method were determined using urine based quality control samples, and matrix effects were examined by using standard addition methods. This novel method is suitable for elucidating differences in urinary polyamine excretion in cancer patients and healthy humans.",
keywords = "Cancer diagnostic markers, Liquid chromatography-tandem mass spectrometry, N-acetylated, Polyamines, Prostate cancer, Urine",
author = "H{\"a}kkinen, {Merja R.} and Antti Roine and Seppo Auriola and Antti Tuokko and Erik Veskim{\"a}e and Kein{\"a}nen, {Tuomo A.} and Terho Lehtim{\"a}ki and Niku Oksala and Jouko Veps{\"a}l{\"a}inen",
year = "2013",
month = "12",
day = "15",
doi = "10.1016/j.jchromb.2013.10.009",
language = "English",
volume = "941",
pages = "81--89",
journal = "JOURNAL OF CHROMATOGRAPHY B: ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES",
issn = "1570-0232",
publisher = "Elsevier",

}

RIS (suitable for import to EndNote) - Lataa

TY - JOUR

T1 - Analysis of free, mono- and diacetylated polyamines from human urine by LC-MS/MS

AU - Häkkinen, Merja R.

AU - Roine, Antti

AU - Auriola, Seppo

AU - Tuokko, Antti

AU - Veskimäe, Erik

AU - Keinänen, Tuomo A.

AU - Lehtimäki, Terho

AU - Oksala, Niku

AU - Vepsäläinen, Jouko

PY - 2013/12/15

Y1 - 2013/12/15

N2 - Polyamines are promising biochemical markers of cancer and many other pathophysiological conditions, and thus their concentrations in biological fluids are a matter of interest. However, since the concentrations of these compounds are low, their quantitation is typically based on methods requiring laborious sample preparation. Here we developed and validated an LC-MS/MS method to analyze simultaneously free (DAP, PUT, CAD, SPD, SPM) monoacetylated (AcPUT, AcCAD, N1AcSPD, N8AcSPD, N1AcSPM) and diacetylated (DiAcPUT, DiAcCAD, DiAcSPD, DiAcSPM) polyamines from human urine without the need for derivatization. Deuterium labeled polyamines were the internal standards for each analyte. Diluted urine samples spiked with internal standards were filtered through a strong anion exchange resin prior to LC-MS/MS analysis. The chromatographic separation of 14 polyamines was achieved in 12min on C18 column with 0.1% HFBA (v/v) as the ion-pairing agent and a water-acetonitrile gradient. Ionization was performed with positive electrospray ionization (ESI) and detection was with a triple quadrupole mass spectrometer with selected reaction monitoring. Calibration curves ranged from up to 5 to 10,000nM. The accuracy and precision of the method were determined using urine based quality control samples, and matrix effects were examined by using standard addition methods. This novel method is suitable for elucidating differences in urinary polyamine excretion in cancer patients and healthy humans.

AB - Polyamines are promising biochemical markers of cancer and many other pathophysiological conditions, and thus their concentrations in biological fluids are a matter of interest. However, since the concentrations of these compounds are low, their quantitation is typically based on methods requiring laborious sample preparation. Here we developed and validated an LC-MS/MS method to analyze simultaneously free (DAP, PUT, CAD, SPD, SPM) monoacetylated (AcPUT, AcCAD, N1AcSPD, N8AcSPD, N1AcSPM) and diacetylated (DiAcPUT, DiAcCAD, DiAcSPD, DiAcSPM) polyamines from human urine without the need for derivatization. Deuterium labeled polyamines were the internal standards for each analyte. Diluted urine samples spiked with internal standards were filtered through a strong anion exchange resin prior to LC-MS/MS analysis. The chromatographic separation of 14 polyamines was achieved in 12min on C18 column with 0.1% HFBA (v/v) as the ion-pairing agent and a water-acetonitrile gradient. Ionization was performed with positive electrospray ionization (ESI) and detection was with a triple quadrupole mass spectrometer with selected reaction monitoring. Calibration curves ranged from up to 5 to 10,000nM. The accuracy and precision of the method were determined using urine based quality control samples, and matrix effects were examined by using standard addition methods. This novel method is suitable for elucidating differences in urinary polyamine excretion in cancer patients and healthy humans.

KW - Cancer diagnostic markers

KW - Liquid chromatography-tandem mass spectrometry

KW - N-acetylated

KW - Polyamines

KW - Prostate cancer

KW - Urine

UR - http://www.scopus.com/inward/record.url?scp=84887097655&partnerID=8YFLogxK

U2 - 10.1016/j.jchromb.2013.10.009

DO - 10.1016/j.jchromb.2013.10.009

M3 - Article

VL - 941

SP - 81

EP - 89

JO - JOURNAL OF CHROMATOGRAPHY B: ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES

JF - JOURNAL OF CHROMATOGRAPHY B: ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES

SN - 1570-0232

ER -