Behavior of 4-hydroxynonenal in phospholipid membranes
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Yksityiskohdat
| Alkuperäiskieli | Englanti |
|---|---|
| Sivut | 6411-6415 |
| Sivumäärä | 5 |
| Julkaisu | Journal of Physical Chemistry Part B |
| Vuosikerta | 116 |
| Numero | 22 |
| DOI - pysyväislinkit | |
| Tila | Julkaistu - 7 kesäkuuta 2012 |
| OKM-julkaisutyyppi | A1 Alkuperäisartikkeli |
Tiivistelmä
Under conditions of oxidative stress, 4-hydroxy-2-nonenal (4-HNE) is commonly present in vivo. This highly reactive and cytotoxic compound is generated by oxidation of lipids in membranes and can be easily transferred from a membrane to both cytosol and the extracellular space. Employing time-dependent fluorescence shift (TDFS) method and molecular dynamics simulations, we found that 4-HNE is stabilized in the carbonyl region of a 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) bilayer. 4-HNE is thus able to react with cell membrane proteins and lipids. Stabilization in the membrane is, however, moderate and a transfer of 4-HNE to either extra- or intracellular space occurs on a microsecond time scale. These molecular-level details of 4-HNE behavior in the lipid membrane rationalize the experimentally observed reactivity of 4-HNE with proteins inside and outside the cell. Furthermore, these results support the view that 4-HNE may play an active role in cell signaling pathways.