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Bioactive glass ions induce efficient osteogenic differentiation of human adipose stem cells encapsulated in gellan gum and collagen type I hydrogels

Tutkimustuotosvertaisarvioitu

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Bioactive glass ions induce efficient osteogenic differentiation of human adipose stem cells encapsulated in gellan gum and collagen type I hydrogels. / Vuornos, Kaisa; Ojansivu, Miina; Koivisto, Janne T.; Häkkänen, Heikki; Belay, Birhanu; Montonen, Toni; Huhtala, Heini; Kääriäinen, Minna; Hupa, Leena; Kellomäki, Minna; Hyttinen, Jari; Ihalainen, Janne A.; Miettinen, Susanna.

julkaisussa: Materials Science and Engineering C, Vuosikerta 99, 01.06.2019, s. 905-918.

Tutkimustuotosvertaisarvioitu

Harvard

Vuornos, K, Ojansivu, M, Koivisto, JT, Häkkänen, H, Belay, B, Montonen, T, Huhtala, H, Kääriäinen, M, Hupa, L, Kellomäki, M, Hyttinen, J, Ihalainen, JA & Miettinen, S 2019, 'Bioactive glass ions induce efficient osteogenic differentiation of human adipose stem cells encapsulated in gellan gum and collagen type I hydrogels', Materials Science and Engineering C, Vuosikerta. 99, Sivut 905-918. https://doi.org/10.1016/j.msec.2019.02.035

APA

Vuornos, K., Ojansivu, M., Koivisto, J. T., Häkkänen, H., Belay, B., Montonen, T., ... Miettinen, S. (2019). Bioactive glass ions induce efficient osteogenic differentiation of human adipose stem cells encapsulated in gellan gum and collagen type I hydrogels. Materials Science and Engineering C, 99, 905-918. https://doi.org/10.1016/j.msec.2019.02.035

Vancouver

Author

Vuornos, Kaisa ; Ojansivu, Miina ; Koivisto, Janne T. ; Häkkänen, Heikki ; Belay, Birhanu ; Montonen, Toni ; Huhtala, Heini ; Kääriäinen, Minna ; Hupa, Leena ; Kellomäki, Minna ; Hyttinen, Jari ; Ihalainen, Janne A. ; Miettinen, Susanna. / Bioactive glass ions induce efficient osteogenic differentiation of human adipose stem cells encapsulated in gellan gum and collagen type I hydrogels. Julkaisussa: Materials Science and Engineering C. 2019 ; Vuosikerta 99. Sivut 905-918.

Bibtex - Lataa

@article{5bea8cb635f5494d935b37911ef89e67,
title = "Bioactive glass ions induce efficient osteogenic differentiation of human adipose stem cells encapsulated in gellan gum and collagen type I hydrogels",
abstract = "Background: Due to unmet need for bone augmentation, our aim was to promote osteogenic differentiation of human adipose stem cells (hASCs) encapsulated in gellan gum (GG) or collagen type I (COL) hydrogels with bioactive glass (experimental glass 2-06 of composition [wt-{\%}]: Na 2 O 12.1, K 2 O 14.0, CaO 19.8, P 2 O5 2.5, B 2 O 3 1.6, SiO 2 50.0) extract based osteogenic medium (BaG OM) for bone construct development. GG hydrogels were crosslinked with spermidine (GG-SPD) or BaG extract (GG-BaG). Methods: Mechanical properties of cell-free GG-SPD, GG-BaG, and COL hydrogels were tested in osteogenic medium (OM) or BaG OM at 0, 14, and 21 d. Hydrogel embedded hASCs were cultured in OM or BaG OM for 3, 14, and 21 d, and analyzed for viability, cell number, osteogenic gene expression, osteocalcin production, and mineralization. Hydroxyapatite-stained GG-SPD samples were imaged with Optical Projection Tomography (OPT) and Selective Plane Illumination Microscopy (SPIM) in OM and BaG OM at 21 d. Furthermore, Raman spectroscopy was used to study the calcium phosphate (CaP) content of hASC-secreted ECM in GG-SPD, GG-BaG, and COL at 21 d in BaG OM. Results: The results showed viable rounded cells in GG whereas hASCs were elongated in COL. Importantly, BaG OM induced significantly higher cell number and higher osteogenic gene expression in COL. In both hydrogels, BaG OM induced strong mineralization confirmed as CaP by Raman spectroscopy and significantly improved mechanical properties. GG-BaG hydrogels rescued hASC mineralization in OM. OPT and SPIM showed homogeneous 3D cell distribution with strong mineralization in BaG OM. Also, strong osteocalcin production was visible in COL. Conclusions: Overall, we showed efficacious osteogenesis of hASCs in 3D hydrogels with BaG OM with potential for bone-like grafts.",
keywords = "Adipose stem cell, Bioactive glass, Collagen type I hydrogel, Gellan gum hydrogel, Osteogenic differentiation",
author = "Kaisa Vuornos and Miina Ojansivu and Koivisto, {Janne T.} and Heikki H{\"a}kk{\"a}nen and Birhanu Belay and Toni Montonen and Heini Huhtala and Minna K{\"a}{\"a}ri{\"a}inen and Leena Hupa and Minna Kellom{\"a}ki and Jari Hyttinen and Ihalainen, {Janne A.} and Susanna Miettinen",
note = "DUPL=47148426",
year = "2019",
month = "6",
day = "1",
doi = "10.1016/j.msec.2019.02.035",
language = "English",
volume = "99",
pages = "905--918",
journal = "Materials Science and Engineering C: Materials for Biological Applications",
issn = "0928-4931",
publisher = "Elsevier",

}

RIS (suitable for import to EndNote) - Lataa

TY - JOUR

T1 - Bioactive glass ions induce efficient osteogenic differentiation of human adipose stem cells encapsulated in gellan gum and collagen type I hydrogels

AU - Vuornos, Kaisa

AU - Ojansivu, Miina

AU - Koivisto, Janne T.

AU - Häkkänen, Heikki

AU - Belay, Birhanu

AU - Montonen, Toni

AU - Huhtala, Heini

AU - Kääriäinen, Minna

AU - Hupa, Leena

AU - Kellomäki, Minna

AU - Hyttinen, Jari

AU - Ihalainen, Janne A.

AU - Miettinen, Susanna

N1 - DUPL=47148426

PY - 2019/6/1

Y1 - 2019/6/1

N2 - Background: Due to unmet need for bone augmentation, our aim was to promote osteogenic differentiation of human adipose stem cells (hASCs) encapsulated in gellan gum (GG) or collagen type I (COL) hydrogels with bioactive glass (experimental glass 2-06 of composition [wt-%]: Na 2 O 12.1, K 2 O 14.0, CaO 19.8, P 2 O5 2.5, B 2 O 3 1.6, SiO 2 50.0) extract based osteogenic medium (BaG OM) for bone construct development. GG hydrogels were crosslinked with spermidine (GG-SPD) or BaG extract (GG-BaG). Methods: Mechanical properties of cell-free GG-SPD, GG-BaG, and COL hydrogels were tested in osteogenic medium (OM) or BaG OM at 0, 14, and 21 d. Hydrogel embedded hASCs were cultured in OM or BaG OM for 3, 14, and 21 d, and analyzed for viability, cell number, osteogenic gene expression, osteocalcin production, and mineralization. Hydroxyapatite-stained GG-SPD samples were imaged with Optical Projection Tomography (OPT) and Selective Plane Illumination Microscopy (SPIM) in OM and BaG OM at 21 d. Furthermore, Raman spectroscopy was used to study the calcium phosphate (CaP) content of hASC-secreted ECM in GG-SPD, GG-BaG, and COL at 21 d in BaG OM. Results: The results showed viable rounded cells in GG whereas hASCs were elongated in COL. Importantly, BaG OM induced significantly higher cell number and higher osteogenic gene expression in COL. In both hydrogels, BaG OM induced strong mineralization confirmed as CaP by Raman spectroscopy and significantly improved mechanical properties. GG-BaG hydrogels rescued hASC mineralization in OM. OPT and SPIM showed homogeneous 3D cell distribution with strong mineralization in BaG OM. Also, strong osteocalcin production was visible in COL. Conclusions: Overall, we showed efficacious osteogenesis of hASCs in 3D hydrogels with BaG OM with potential for bone-like grafts.

AB - Background: Due to unmet need for bone augmentation, our aim was to promote osteogenic differentiation of human adipose stem cells (hASCs) encapsulated in gellan gum (GG) or collagen type I (COL) hydrogels with bioactive glass (experimental glass 2-06 of composition [wt-%]: Na 2 O 12.1, K 2 O 14.0, CaO 19.8, P 2 O5 2.5, B 2 O 3 1.6, SiO 2 50.0) extract based osteogenic medium (BaG OM) for bone construct development. GG hydrogels were crosslinked with spermidine (GG-SPD) or BaG extract (GG-BaG). Methods: Mechanical properties of cell-free GG-SPD, GG-BaG, and COL hydrogels were tested in osteogenic medium (OM) or BaG OM at 0, 14, and 21 d. Hydrogel embedded hASCs were cultured in OM or BaG OM for 3, 14, and 21 d, and analyzed for viability, cell number, osteogenic gene expression, osteocalcin production, and mineralization. Hydroxyapatite-stained GG-SPD samples were imaged with Optical Projection Tomography (OPT) and Selective Plane Illumination Microscopy (SPIM) in OM and BaG OM at 21 d. Furthermore, Raman spectroscopy was used to study the calcium phosphate (CaP) content of hASC-secreted ECM in GG-SPD, GG-BaG, and COL at 21 d in BaG OM. Results: The results showed viable rounded cells in GG whereas hASCs were elongated in COL. Importantly, BaG OM induced significantly higher cell number and higher osteogenic gene expression in COL. In both hydrogels, BaG OM induced strong mineralization confirmed as CaP by Raman spectroscopy and significantly improved mechanical properties. GG-BaG hydrogels rescued hASC mineralization in OM. OPT and SPIM showed homogeneous 3D cell distribution with strong mineralization in BaG OM. Also, strong osteocalcin production was visible in COL. Conclusions: Overall, we showed efficacious osteogenesis of hASCs in 3D hydrogels with BaG OM with potential for bone-like grafts.

KW - Adipose stem cell

KW - Bioactive glass

KW - Collagen type I hydrogel

KW - Gellan gum hydrogel

KW - Osteogenic differentiation

U2 - 10.1016/j.msec.2019.02.035

DO - 10.1016/j.msec.2019.02.035

M3 - Article

VL - 99

SP - 905

EP - 918

JO - Materials Science and Engineering C: Materials for Biological Applications

JF - Materials Science and Engineering C: Materials for Biological Applications

SN - 0928-4931

ER -