Bioprocessing of enhanced cellulase production from a mutant of Trichoderma asperellum RCK2011 and its application in hydrolysis of cellulose

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Bioprocessing of enhanced cellulase production from a mutant of Trichoderma asperellum RCK2011 and its application in hydrolysis of cellulose. / Raghuwanshi, Sanjeev; Deswal, Deepa; Karp, Matti; Kuhad, Ramesh Chander.

julkaisussa: Fuel, Vuosikerta 124, 15.05.2014, s. 183-189.

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Bibtex - Lataa

@article{50e57c1d6eae47f3aad485e66cf52fa6,

title = "Bioprocessing of enhanced cellulase production from a mutant of Trichoderma asperellum RCK2011 and its application in hydrolysis of cellulose",

abstract = "A mutant strain of Trichoderma asperellum RCK2011 was developed through UV-irradiation for enhanced cellulase production and lower catabolite repression. The production of FPase, CMCase and β-glucosidase was optimized under solid state fermentation; up to 20 mM of glucose did not inhibit cellulase production. The mutant strain T. asperellum SR1-7 produced FPase (2.2 IU/gds), CMCase (13.2 IU/gds), and β-glucosidase (9.2 IU/gds) under optimized conditions, which is, 1.4, 1.3, 1.5-fold higher than the wild type. The wild as well as mutant strain produced the cellulases at pH range, 4.0-10.0. Saccharification of pretreated corn cob, wheat straw, and sugarcane bagasse by cellulase from mutant strain SR1-7 resulted in release of reducing sugar at the rate of 530.0 mg/g, 290.0 mg/g, and 335.0 mg/g of substrate, respectively; this is 1.6-fold higher than the wild type strain. {\circledC} 2014 Published by Elsevier Ltd.",

keywords = "Alkaline cellulase, Catabolite repression, Saccharification, Solid state fermentation",

author = "Sanjeev Raghuwanshi and Deepa Deswal and Matti Karp and Kuhad, {Ramesh Chander}",

note = "Contribution: organisation=keb,FACT1=1<br/>Portfolio EDEND: 2014-03-15",

year = "2014",

month = "5",

day = "15",

doi = "10.1016/j.fuel.2014.01.107",

language = "English",

volume = "124",

pages = "183--189",

journal = "Fuel",

issn = "0016-2361",

publisher = "ELSEVIER SCI LTD",

}

RIS (suitable for import to EndNote) - Lataa

TY - JOUR

T1 - Bioprocessing of enhanced cellulase production from a mutant of Trichoderma asperellum RCK2011 and its application in hydrolysis of cellulose

AU - Raghuwanshi, Sanjeev

AU - Deswal, Deepa

AU - Karp, Matti

AU - Kuhad, Ramesh Chander

N1 - Contribution: organisation=keb,FACT1=1<br/>Portfolio EDEND: 2014-03-15

PY - 2014/5/15

Y1 - 2014/5/15

N2 - A mutant strain of Trichoderma asperellum RCK2011 was developed through UV-irradiation for enhanced cellulase production and lower catabolite repression. The production of FPase, CMCase and β-glucosidase was optimized under solid state fermentation; up to 20 mM of glucose did not inhibit cellulase production. The mutant strain T. asperellum SR1-7 produced FPase (2.2 IU/gds), CMCase (13.2 IU/gds), and β-glucosidase (9.2 IU/gds) under optimized conditions, which is, 1.4, 1.3, 1.5-fold higher than the wild type. The wild as well as mutant strain produced the cellulases at pH range, 4.0-10.0. Saccharification of pretreated corn cob, wheat straw, and sugarcane bagasse by cellulase from mutant strain SR1-7 resulted in release of reducing sugar at the rate of 530.0 mg/g, 290.0 mg/g, and 335.0 mg/g of substrate, respectively; this is 1.6-fold higher than the wild type strain. © 2014 Published by Elsevier Ltd.

AB - A mutant strain of Trichoderma asperellum RCK2011 was developed through UV-irradiation for enhanced cellulase production and lower catabolite repression. The production of FPase, CMCase and β-glucosidase was optimized under solid state fermentation; up to 20 mM of glucose did not inhibit cellulase production. The mutant strain T. asperellum SR1-7 produced FPase (2.2 IU/gds), CMCase (13.2 IU/gds), and β-glucosidase (9.2 IU/gds) under optimized conditions, which is, 1.4, 1.3, 1.5-fold higher than the wild type. The wild as well as mutant strain produced the cellulases at pH range, 4.0-10.0. Saccharification of pretreated corn cob, wheat straw, and sugarcane bagasse by cellulase from mutant strain SR1-7 resulted in release of reducing sugar at the rate of 530.0 mg/g, 290.0 mg/g, and 335.0 mg/g of substrate, respectively; this is 1.6-fold higher than the wild type strain. © 2014 Published by Elsevier Ltd.

KW - Alkaline cellulase

KW - Catabolite repression

KW - Saccharification

KW - Solid state fermentation

UR - http://www.scopus.com/inward/record.url?scp=84894571819&partnerID=8YFLogxK

U2 - 10.1016/j.fuel.2014.01.107

DO - 10.1016/j.fuel.2014.01.107

M3 - Article

VL - 124

SP - 183

EP - 189

JO - Fuel

JF - Fuel

SN - 0016-2361

ER -

TUTCRIS