Bioprocessing of enhanced cellulase production from a mutant of Trichoderma asperellum RCK2011 and its application in hydrolysis of cellulose
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Bioprocessing of enhanced cellulase production from a mutant of Trichoderma asperellum RCK2011 and its application in hydrolysis of cellulose. / Raghuwanshi, Sanjeev; Deswal, Deepa; Karp, Matti; Kuhad, Ramesh Chander.
julkaisussa: Fuel, Vuosikerta 124, 15.05.2014, s. 183-189.Tutkimustuotos › › vertaisarvioitu
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TY - JOUR
T1 - Bioprocessing of enhanced cellulase production from a mutant of Trichoderma asperellum RCK2011 and its application in hydrolysis of cellulose
AU - Raghuwanshi, Sanjeev
AU - Deswal, Deepa
AU - Karp, Matti
AU - Kuhad, Ramesh Chander
N1 - Contribution: organisation=keb,FACT1=1<br/>Portfolio EDEND: 2014-03-15
PY - 2014/5/15
Y1 - 2014/5/15
N2 - A mutant strain of Trichoderma asperellum RCK2011 was developed through UV-irradiation for enhanced cellulase production and lower catabolite repression. The production of FPase, CMCase and β-glucosidase was optimized under solid state fermentation; up to 20 mM of glucose did not inhibit cellulase production. The mutant strain T. asperellum SR1-7 produced FPase (2.2 IU/gds), CMCase (13.2 IU/gds), and β-glucosidase (9.2 IU/gds) under optimized conditions, which is, 1.4, 1.3, 1.5-fold higher than the wild type. The wild as well as mutant strain produced the cellulases at pH range, 4.0-10.0. Saccharification of pretreated corn cob, wheat straw, and sugarcane bagasse by cellulase from mutant strain SR1-7 resulted in release of reducing sugar at the rate of 530.0 mg/g, 290.0 mg/g, and 335.0 mg/g of substrate, respectively; this is 1.6-fold higher than the wild type strain. © 2014 Published by Elsevier Ltd.
AB - A mutant strain of Trichoderma asperellum RCK2011 was developed through UV-irradiation for enhanced cellulase production and lower catabolite repression. The production of FPase, CMCase and β-glucosidase was optimized under solid state fermentation; up to 20 mM of glucose did not inhibit cellulase production. The mutant strain T. asperellum SR1-7 produced FPase (2.2 IU/gds), CMCase (13.2 IU/gds), and β-glucosidase (9.2 IU/gds) under optimized conditions, which is, 1.4, 1.3, 1.5-fold higher than the wild type. The wild as well as mutant strain produced the cellulases at pH range, 4.0-10.0. Saccharification of pretreated corn cob, wheat straw, and sugarcane bagasse by cellulase from mutant strain SR1-7 resulted in release of reducing sugar at the rate of 530.0 mg/g, 290.0 mg/g, and 335.0 mg/g of substrate, respectively; this is 1.6-fold higher than the wild type strain. © 2014 Published by Elsevier Ltd.
KW - Alkaline cellulase
KW - Catabolite repression
KW - Saccharification
KW - Solid state fermentation
UR - http://www.scopus.com/inward/record.url?scp=84894571819&partnerID=8YFLogxK
U2 - 10.1016/j.fuel.2014.01.107
DO - 10.1016/j.fuel.2014.01.107
M3 - Article
VL - 124
SP - 183
EP - 189
JO - Fuel
JF - Fuel
SN - 0016-2361
ER -