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TUTCRIS

Carbon nanotube doped pericardial matrix derived electroconductive biohybrid hydrogel for cardiac tissue engineering

Tutkimustuotosvertaisarvioitu

Standard

Carbon nanotube doped pericardial matrix derived electroconductive biohybrid hydrogel for cardiac tissue engineering. / Roshanbinfar, Kaveh; Mohammadi, Zahra; Sheikh-Mahdi Mesgar, Abdorreza; Dehghan, Mohammad Mehdi; Oommen, Oommen P.; Hilborn, Jöns; Engel, Felix B.

julkaisussa: Biomaterials Science, Vuosikerta 7, Nro 9, 01.09.2019, s. 3906-3917.

Tutkimustuotosvertaisarvioitu

Harvard

Roshanbinfar, K, Mohammadi, Z, Sheikh-Mahdi Mesgar, A, Dehghan, MM, Oommen, OP, Hilborn, J & Engel, FB 2019, 'Carbon nanotube doped pericardial matrix derived electroconductive biohybrid hydrogel for cardiac tissue engineering', Biomaterials Science, Vuosikerta. 7, Nro 9, Sivut 3906-3917. https://doi.org/10.1039/c9bm00434c

APA

Roshanbinfar, K., Mohammadi, Z., Sheikh-Mahdi Mesgar, A., Dehghan, M. M., Oommen, O. P., Hilborn, J., & Engel, F. B. (2019). Carbon nanotube doped pericardial matrix derived electroconductive biohybrid hydrogel for cardiac tissue engineering. Biomaterials Science, 7(9), 3906-3917. https://doi.org/10.1039/c9bm00434c

Vancouver

Roshanbinfar K, Mohammadi Z, Sheikh-Mahdi Mesgar A, Dehghan MM, Oommen OP, Hilborn J et al. Carbon nanotube doped pericardial matrix derived electroconductive biohybrid hydrogel for cardiac tissue engineering. Biomaterials Science. 2019 syys 1;7(9):3906-3917. https://doi.org/10.1039/c9bm00434c

Author

Roshanbinfar, Kaveh ; Mohammadi, Zahra ; Sheikh-Mahdi Mesgar, Abdorreza ; Dehghan, Mohammad Mehdi ; Oommen, Oommen P. ; Hilborn, Jöns ; Engel, Felix B. / Carbon nanotube doped pericardial matrix derived electroconductive biohybrid hydrogel for cardiac tissue engineering. Julkaisussa: Biomaterials Science. 2019 ; Vuosikerta 7, Nro 9. Sivut 3906-3917.

Bibtex - Lataa

@article{9ba1bca8db2d42db9411df81fb417a96,
title = "Carbon nanotube doped pericardial matrix derived electroconductive biohybrid hydrogel for cardiac tissue engineering",
abstract = "Cardiovascular diseases represent a major socio-economic burden. In recent years, considerable effort has been invested in optimizing cell delivery strategies to advance cell transplantation therapies to restore heart function for example after an infarct. A particular issue is that the implantation of cells using a non-electroconductive matrix potentially causes arrhythmia. Here, we demonstrate that our hydrazide-functionalized nanotubes-pericardial matrix-derived electroconductive biohybrid hydrogel provides a suitable environment for maturation of human-induced pluripotent stem cell (hiPSC)-derived cardiomyocytes. hiPSC-derived cardiomyocytes exhibited an improved contraction amplitude (>500{\%}) on conductive hydrogels compared to cells cultured on Matrigel{\circledR}. This was accompanied by increased cellular alignment, enhanced connexin 43 expression, and improved sarcomere organization suggesting maturation of the hiPSC-derived cardiomyocytes. Sarcomeric length of these cells increased from 1.3 to 1.7 μm. Moreover, 3D cell-laden engineered tissues exhibited enhanced calcium handling as well as positive response to external electrical and pharmaceutical stimulation. Collectively, our data indicate that our biohybrid hydrogels consisting of solubilized nanostructured pericardial matrix and electroconductive positively charged hydrazide-conjugated carbon nanotubes provide a promising material for stem cell-based cardiac tissue engineering.",
author = "Kaveh Roshanbinfar and Zahra Mohammadi and {Sheikh-Mahdi Mesgar}, Abdorreza and Dehghan, {Mohammad Mehdi} and Oommen, {Oommen P.} and J{\"o}ns Hilborn and Engel, {Felix B.}",
year = "2019",
month = "9",
day = "1",
doi = "10.1039/c9bm00434c",
language = "English",
volume = "7",
pages = "3906--3917",
journal = "Biomaterials Science",
issn = "2047-4830",
publisher = "Royal Society of Chemistry",
number = "9",

}

RIS (suitable for import to EndNote) - Lataa

TY - JOUR

T1 - Carbon nanotube doped pericardial matrix derived electroconductive biohybrid hydrogel for cardiac tissue engineering

AU - Roshanbinfar, Kaveh

AU - Mohammadi, Zahra

AU - Sheikh-Mahdi Mesgar, Abdorreza

AU - Dehghan, Mohammad Mehdi

AU - Oommen, Oommen P.

AU - Hilborn, Jöns

AU - Engel, Felix B.

PY - 2019/9/1

Y1 - 2019/9/1

N2 - Cardiovascular diseases represent a major socio-economic burden. In recent years, considerable effort has been invested in optimizing cell delivery strategies to advance cell transplantation therapies to restore heart function for example after an infarct. A particular issue is that the implantation of cells using a non-electroconductive matrix potentially causes arrhythmia. Here, we demonstrate that our hydrazide-functionalized nanotubes-pericardial matrix-derived electroconductive biohybrid hydrogel provides a suitable environment for maturation of human-induced pluripotent stem cell (hiPSC)-derived cardiomyocytes. hiPSC-derived cardiomyocytes exhibited an improved contraction amplitude (>500%) on conductive hydrogels compared to cells cultured on Matrigel®. This was accompanied by increased cellular alignment, enhanced connexin 43 expression, and improved sarcomere organization suggesting maturation of the hiPSC-derived cardiomyocytes. Sarcomeric length of these cells increased from 1.3 to 1.7 μm. Moreover, 3D cell-laden engineered tissues exhibited enhanced calcium handling as well as positive response to external electrical and pharmaceutical stimulation. Collectively, our data indicate that our biohybrid hydrogels consisting of solubilized nanostructured pericardial matrix and electroconductive positively charged hydrazide-conjugated carbon nanotubes provide a promising material for stem cell-based cardiac tissue engineering.

AB - Cardiovascular diseases represent a major socio-economic burden. In recent years, considerable effort has been invested in optimizing cell delivery strategies to advance cell transplantation therapies to restore heart function for example after an infarct. A particular issue is that the implantation of cells using a non-electroconductive matrix potentially causes arrhythmia. Here, we demonstrate that our hydrazide-functionalized nanotubes-pericardial matrix-derived electroconductive biohybrid hydrogel provides a suitable environment for maturation of human-induced pluripotent stem cell (hiPSC)-derived cardiomyocytes. hiPSC-derived cardiomyocytes exhibited an improved contraction amplitude (>500%) on conductive hydrogels compared to cells cultured on Matrigel®. This was accompanied by increased cellular alignment, enhanced connexin 43 expression, and improved sarcomere organization suggesting maturation of the hiPSC-derived cardiomyocytes. Sarcomeric length of these cells increased from 1.3 to 1.7 μm. Moreover, 3D cell-laden engineered tissues exhibited enhanced calcium handling as well as positive response to external electrical and pharmaceutical stimulation. Collectively, our data indicate that our biohybrid hydrogels consisting of solubilized nanostructured pericardial matrix and electroconductive positively charged hydrazide-conjugated carbon nanotubes provide a promising material for stem cell-based cardiac tissue engineering.

U2 - 10.1039/c9bm00434c

DO - 10.1039/c9bm00434c

M3 - Article

VL - 7

SP - 3906

EP - 3917

JO - Biomaterials Science

JF - Biomaterials Science

SN - 2047-4830

IS - 9

ER -