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Changes in global gene expression of Vibrio parahaemolyticus induced by cold- and heat-stress

Tutkimustuotosvertaisarvioitu

Standard

Changes in global gene expression of Vibrio parahaemolyticus induced by cold- and heat-stress. / Urmersbach, Sara; Aho, Tommi; Alter, Thomas; Hassan, Syeda Sakira; Autio, Reija; Huehn, Stephan.

julkaisussa: BMC Microbiology, Vuosikerta 15, Nro 1, 229, 23.10.2015.

Tutkimustuotosvertaisarvioitu

Harvard

Urmersbach, S, Aho, T, Alter, T, Hassan, SS, Autio, R & Huehn, S 2015, 'Changes in global gene expression of Vibrio parahaemolyticus induced by cold- and heat-stress', BMC Microbiology, Vuosikerta. 15, Nro 1, 229. https://doi.org/10.1186/s12866-015-0565-7

APA

Urmersbach, S., Aho, T., Alter, T., Hassan, S. S., Autio, R., & Huehn, S. (2015). Changes in global gene expression of Vibrio parahaemolyticus induced by cold- and heat-stress. BMC Microbiology, 15(1), [229]. https://doi.org/10.1186/s12866-015-0565-7

Vancouver

Urmersbach S, Aho T, Alter T, Hassan SS, Autio R, Huehn S. Changes in global gene expression of Vibrio parahaemolyticus induced by cold- and heat-stress. BMC Microbiology. 2015 loka 23;15(1). 229. https://doi.org/10.1186/s12866-015-0565-7

Author

Urmersbach, Sara ; Aho, Tommi ; Alter, Thomas ; Hassan, Syeda Sakira ; Autio, Reija ; Huehn, Stephan. / Changes in global gene expression of Vibrio parahaemolyticus induced by cold- and heat-stress. Julkaisussa: BMC Microbiology. 2015 ; Vuosikerta 15, Nro 1.

Bibtex - Lataa

@article{cb6fcb8e2f3049e69bcca4eed34a6ef2,
title = "Changes in global gene expression of Vibrio parahaemolyticus induced by cold- and heat-stress",
abstract = "Background: Vibrio (V.) parahaemolyticus causes seafood-borne gastro-intestinal bacterial infections in humans worldwide. It is widely found in marine environments and is isolated frequently from seawater, estuarine waters, sediments and raw or insufficiently cooked seafood. Throughout the food chain, V. parahaemolyticus encounters different temperature conditions that might alter metabolism and pathogenicity of the bacterium. In this study, we performed gene expression profiling of V. parahaemolyticus RIMD 2210633 after exposure to 4, 15, 20, 37 and 42°C to describe the cold and heat shock response. Methods: Gene expression profiles of V. parahaemolyticus RIMD 2210633 after exposure to 4, 15, 20, 37 and 42°C were investigated via microarray. Gene expression values and RT-qPCR experiments were compared by plotting the log2 values. Moreover, volcano plots of microarray data were calculated to visualize the distribution of differentially expressed genes at individual temperatures and to assess hybridization qualities and comparability of data. Finally, enriched terms were searched in annotations as well as functional-related gene categories using the Database for Annotation, Visualization and Integrated Discovery. Results: Analysis of 37°C normalised transcriptomics data resulted in differential expression of 19 genes at 20°C, 193 genes at 4°C, 625 genes at 42°C and 638 genes at 15°C. Thus, the largest number of significantly expressed genes was observed at 15 and 42°C with 13.3 and 13 {\%}, respectively. Genes of many functional categories were highly regulated even at lower temperatures. Virulence associated genes (tdh1, tdh2, toxR, toxS, vopC, T6SS-1, T6SS-2) remained mostly unaffected by heat or cold stress. Conclusion: Along with folding and temperature shock depending systems, an overall temperature-dependent regulation of expression could be shown. Particularly the energy metabolism was affected by changed temperatures. Whole-genome gene expression studies of food related pathogens such as V. parahaemolyticus reveal how these pathogens react to stress impacts to predict its behaviour under conditions like storage and transport.",
keywords = "Gene expression, Thermal shock, Vibrio parahaemolyticus",
author = "Sara Urmersbach and Tommi Aho and Thomas Alter and Hassan, {Syeda Sakira} and Reija Autio and Stephan Huehn",
note = "ORG=keb,0.5 ORG=sgn,0.5",
year = "2015",
month = "10",
day = "23",
doi = "10.1186/s12866-015-0565-7",
language = "English",
volume = "15",
journal = "BMC Microbiology",
issn = "1471-2180",
publisher = "Springer Verlag",
number = "1",

}

RIS (suitable for import to EndNote) - Lataa

TY - JOUR

T1 - Changes in global gene expression of Vibrio parahaemolyticus induced by cold- and heat-stress

AU - Urmersbach, Sara

AU - Aho, Tommi

AU - Alter, Thomas

AU - Hassan, Syeda Sakira

AU - Autio, Reija

AU - Huehn, Stephan

N1 - ORG=keb,0.5 ORG=sgn,0.5

PY - 2015/10/23

Y1 - 2015/10/23

N2 - Background: Vibrio (V.) parahaemolyticus causes seafood-borne gastro-intestinal bacterial infections in humans worldwide. It is widely found in marine environments and is isolated frequently from seawater, estuarine waters, sediments and raw or insufficiently cooked seafood. Throughout the food chain, V. parahaemolyticus encounters different temperature conditions that might alter metabolism and pathogenicity of the bacterium. In this study, we performed gene expression profiling of V. parahaemolyticus RIMD 2210633 after exposure to 4, 15, 20, 37 and 42°C to describe the cold and heat shock response. Methods: Gene expression profiles of V. parahaemolyticus RIMD 2210633 after exposure to 4, 15, 20, 37 and 42°C were investigated via microarray. Gene expression values and RT-qPCR experiments were compared by plotting the log2 values. Moreover, volcano plots of microarray data were calculated to visualize the distribution of differentially expressed genes at individual temperatures and to assess hybridization qualities and comparability of data. Finally, enriched terms were searched in annotations as well as functional-related gene categories using the Database for Annotation, Visualization and Integrated Discovery. Results: Analysis of 37°C normalised transcriptomics data resulted in differential expression of 19 genes at 20°C, 193 genes at 4°C, 625 genes at 42°C and 638 genes at 15°C. Thus, the largest number of significantly expressed genes was observed at 15 and 42°C with 13.3 and 13 %, respectively. Genes of many functional categories were highly regulated even at lower temperatures. Virulence associated genes (tdh1, tdh2, toxR, toxS, vopC, T6SS-1, T6SS-2) remained mostly unaffected by heat or cold stress. Conclusion: Along with folding and temperature shock depending systems, an overall temperature-dependent regulation of expression could be shown. Particularly the energy metabolism was affected by changed temperatures. Whole-genome gene expression studies of food related pathogens such as V. parahaemolyticus reveal how these pathogens react to stress impacts to predict its behaviour under conditions like storage and transport.

AB - Background: Vibrio (V.) parahaemolyticus causes seafood-borne gastro-intestinal bacterial infections in humans worldwide. It is widely found in marine environments and is isolated frequently from seawater, estuarine waters, sediments and raw or insufficiently cooked seafood. Throughout the food chain, V. parahaemolyticus encounters different temperature conditions that might alter metabolism and pathogenicity of the bacterium. In this study, we performed gene expression profiling of V. parahaemolyticus RIMD 2210633 after exposure to 4, 15, 20, 37 and 42°C to describe the cold and heat shock response. Methods: Gene expression profiles of V. parahaemolyticus RIMD 2210633 after exposure to 4, 15, 20, 37 and 42°C were investigated via microarray. Gene expression values and RT-qPCR experiments were compared by plotting the log2 values. Moreover, volcano plots of microarray data were calculated to visualize the distribution of differentially expressed genes at individual temperatures and to assess hybridization qualities and comparability of data. Finally, enriched terms were searched in annotations as well as functional-related gene categories using the Database for Annotation, Visualization and Integrated Discovery. Results: Analysis of 37°C normalised transcriptomics data resulted in differential expression of 19 genes at 20°C, 193 genes at 4°C, 625 genes at 42°C and 638 genes at 15°C. Thus, the largest number of significantly expressed genes was observed at 15 and 42°C with 13.3 and 13 %, respectively. Genes of many functional categories were highly regulated even at lower temperatures. Virulence associated genes (tdh1, tdh2, toxR, toxS, vopC, T6SS-1, T6SS-2) remained mostly unaffected by heat or cold stress. Conclusion: Along with folding and temperature shock depending systems, an overall temperature-dependent regulation of expression could be shown. Particularly the energy metabolism was affected by changed temperatures. Whole-genome gene expression studies of food related pathogens such as V. parahaemolyticus reveal how these pathogens react to stress impacts to predict its behaviour under conditions like storage and transport.

KW - Gene expression

KW - Thermal shock

KW - Vibrio parahaemolyticus

UR - http://www.scopus.com/inward/record.url?scp=84944883751&partnerID=8YFLogxK

U2 - 10.1186/s12866-015-0565-7

DO - 10.1186/s12866-015-0565-7

M3 - Article

VL - 15

JO - BMC Microbiology

JF - BMC Microbiology

SN - 1471-2180

IS - 1

M1 - 229

ER -