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Characterization of endoglucanase rich Trichoderma reesei cellulase mixtures and their effect on alkaline solubility of dissolving pulp

Tutkimustuotosvertaisarvioitu

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Characterization of endoglucanase rich Trichoderma reesei cellulase mixtures and their effect on alkaline solubility of dissolving pulp. / Kamppuri, Taina; Vehviläinen, Marianna; Backfolk, Kaj; Heiskanen, Isto.

julkaisussa: Cellulose, Vuosikerta 23, Nro 6, 12.2016, s. 3901–3911.

Tutkimustuotosvertaisarvioitu

Harvard

Kamppuri, T, Vehviläinen, M, Backfolk, K & Heiskanen, I 2016, 'Characterization of endoglucanase rich Trichoderma reesei cellulase mixtures and their effect on alkaline solubility of dissolving pulp', Cellulose, Vuosikerta. 23, Nro 6, Sivut 3901–3911. https://doi.org/10.1007/s10570-016-1055-2

APA

Vancouver

Author

Kamppuri, Taina ; Vehviläinen, Marianna ; Backfolk, Kaj ; Heiskanen, Isto. / Characterization of endoglucanase rich Trichoderma reesei cellulase mixtures and their effect on alkaline solubility of dissolving pulp. Julkaisussa: Cellulose. 2016 ; Vuosikerta 23, Nro 6. Sivut 3901–3911.

Bibtex - Lataa

@article{eb026d827c6c46869c484bbb9ccf364a,
title = "Characterization of endoglucanase rich Trichoderma reesei cellulase mixtures and their effect on alkaline solubility of dissolving pulp",
abstract = "Dissolving grade pulps are used to manufacture regenerated cellulosic fibres. One promising process for the production of regenerated fibres utilises endoglucanse rich cellulases in the modification of dissolving pulp into alkaline soluble form. The aim of this paper was to characterise cellulases produced by Trichoderma reesei that are available in large quantities and study their effect on the dissolving grade softwood pulp, especially on its alkaline solubility. All the studied cellulases had endoglucanse activity and they decreased the intrinsic viscosity of the pulp. The degradation of cellulose into solubilised sugars increased with the cellulases containing also cellobiohydrolases. The monocomponent endoglucanases enhanced alkaline solubility of the pulp more than the multicomponent cellulases and produced alkaline solutions with higher fluidity. The studies showed that the type of the cellulases in the enzyme mixture has significant effect on the amount of solubilised sugars during the enzyme treatment and on the alkaline solubility of the pulp.",
keywords = "Alkaline solubility, Cellulase, Cellulose, Endoglucanase, Trichoderma reesei",
author = "Taina Kamppuri and Marianna Vehvil{\"a}inen and Kaj Backfolk and Isto Heiskanen",
year = "2016",
month = "12",
doi = "10.1007/s10570-016-1055-2",
language = "English",
volume = "23",
pages = "3901–3911",
journal = "Cellulose",
issn = "0969-0239",
publisher = "Springer Verlag",
number = "6",

}

RIS (suitable for import to EndNote) - Lataa

TY - JOUR

T1 - Characterization of endoglucanase rich Trichoderma reesei cellulase mixtures and their effect on alkaline solubility of dissolving pulp

AU - Kamppuri, Taina

AU - Vehviläinen, Marianna

AU - Backfolk, Kaj

AU - Heiskanen, Isto

PY - 2016/12

Y1 - 2016/12

N2 - Dissolving grade pulps are used to manufacture regenerated cellulosic fibres. One promising process for the production of regenerated fibres utilises endoglucanse rich cellulases in the modification of dissolving pulp into alkaline soluble form. The aim of this paper was to characterise cellulases produced by Trichoderma reesei that are available in large quantities and study their effect on the dissolving grade softwood pulp, especially on its alkaline solubility. All the studied cellulases had endoglucanse activity and they decreased the intrinsic viscosity of the pulp. The degradation of cellulose into solubilised sugars increased with the cellulases containing also cellobiohydrolases. The monocomponent endoglucanases enhanced alkaline solubility of the pulp more than the multicomponent cellulases and produced alkaline solutions with higher fluidity. The studies showed that the type of the cellulases in the enzyme mixture has significant effect on the amount of solubilised sugars during the enzyme treatment and on the alkaline solubility of the pulp.

AB - Dissolving grade pulps are used to manufacture regenerated cellulosic fibres. One promising process for the production of regenerated fibres utilises endoglucanse rich cellulases in the modification of dissolving pulp into alkaline soluble form. The aim of this paper was to characterise cellulases produced by Trichoderma reesei that are available in large quantities and study their effect on the dissolving grade softwood pulp, especially on its alkaline solubility. All the studied cellulases had endoglucanse activity and they decreased the intrinsic viscosity of the pulp. The degradation of cellulose into solubilised sugars increased with the cellulases containing also cellobiohydrolases. The monocomponent endoglucanases enhanced alkaline solubility of the pulp more than the multicomponent cellulases and produced alkaline solutions with higher fluidity. The studies showed that the type of the cellulases in the enzyme mixture has significant effect on the amount of solubilised sugars during the enzyme treatment and on the alkaline solubility of the pulp.

KW - Alkaline solubility

KW - Cellulase

KW - Cellulose

KW - Endoglucanase

KW - Trichoderma reesei

U2 - 10.1007/s10570-016-1055-2

DO - 10.1007/s10570-016-1055-2

M3 - Article

VL - 23

SP - 3901

EP - 3911

JO - Cellulose

JF - Cellulose

SN - 0969-0239

IS - 6

ER -