TUTCRIS - Tampereen teknillinen yliopisto

TUTCRIS

Chimeric avidin shows stability against harsh chemical conditions-biochemical analysis and 3D structure

Tutkimustuotosvertaisarvioitu

Yksityiskohdat

AlkuperäiskieliEnglanti
Sivut481-490
Sivumäärä10
JulkaisuBiotechnology and Bioengineering
Vuosikerta108
Numero3
DOI - pysyväislinkit
TilaJulkaistu - maaliskuuta 2011
OKM-julkaisutyyppiA1 Alkuperäisartikkeli

Tiivistelmä

Avidin and its bacterial analog streptavidin have been widely used in applications in life sciences. Recently, we described a highly thermostable engineered avidin, called chimeric avidin, which is a hybrid of avidin and avidin-related protein 4. Here, we report a protocol for pilot-scale production in E. coli and the X-ray structure of chimeric avidin. The ligand-binding properties of chimeric avidin were explored with isothermal titration calorimetry. We found chimeric avidin to be more stable against various harsh organic solvents at elevated temperatures compared to avidin and streptavidin. The properties of chimeric avidin make it a potential tool for new applications in biotechnology. Biotechnol. Bioeng. 2011; 108:481-490.