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Comparison of three in vitro implant leakage testing methods

Tutkimustuotosvertaisarvioitu

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Comparison of three in vitro implant leakage testing methods. / Al-Jadaa, Anas; Attin, Thomas; Peltomäki, Timo; Schmidlin, Patrick R.

julkaisussa: Clinical Oral Implants Research, Vuosikerta 26, Nro 4, 01.04.2015, s. e1-e7.

Tutkimustuotosvertaisarvioitu

Harvard

Al-Jadaa, A, Attin, T, Peltomäki, T & Schmidlin, PR 2015, 'Comparison of three in vitro implant leakage testing methods', Clinical Oral Implants Research, Vuosikerta. 26, Nro 4, Sivut e1-e7. https://doi.org/10.1111/clr.12314

APA

Al-Jadaa, A., Attin, T., Peltomäki, T., & Schmidlin, P. R. (2015). Comparison of three in vitro implant leakage testing methods. Clinical Oral Implants Research, 26(4), e1-e7. https://doi.org/10.1111/clr.12314

Vancouver

Al-Jadaa A, Attin T, Peltomäki T, Schmidlin PR. Comparison of three in vitro implant leakage testing methods. Clinical Oral Implants Research. 2015 huhti 1;26(4):e1-e7. https://doi.org/10.1111/clr.12314

Author

Al-Jadaa, Anas ; Attin, Thomas ; Peltomäki, Timo ; Schmidlin, Patrick R. / Comparison of three in vitro implant leakage testing methods. Julkaisussa: Clinical Oral Implants Research. 2015 ; Vuosikerta 26, Nro 4. Sivut e1-e7.

Bibtex - Lataa

@article{2cfe67316c6a4c31a7219c144e9db99f,
title = "Comparison of three in vitro implant leakage testing methods",
abstract = "Aims: To assess the accuracy and sensitivity in detecting implants leakage with a gas-enhanced permeation test (GEPT) and to compare with a molecular- and a bacterial-based leakage tests. Materials and methods: Three implants systems were tested (n = 20 per group): Nobel Biocare (NB), Astra Tech (AT) and Biomet 3i (B3i). Implants were mounted in PVC disks and were first tested for gas pressure change and infiltrated saline volume over 40 min. The same implants were then subjected to a molecular leakage evaluation using fluorescent Dextran for 28 days. After cleaning and sterilization, bacterial permeation (E. faecalis) was evaluated by selective media turbidity for another 28 days. Slopes in the pressure change and the perfused saline rate were used as a measure of leakage in the GEPT model and the times of positive events, that is, color change, after molecular and bacterial tests were recorded. Data were analyzed using Kolmogorov-Smirnov/Shapiro-Wilk, Kruskal-Wallis H and Spearman's Rho tests (P <0.05). Results: The gas and saline (ml) leakage values accounted for 0.85 ± 0.71 and 0.56 ± 0.50 ml (AT), 0.23 ± 0.030 and 0.12 ± 0.20 ml (NB) and 0.01 ± 0.01 and 0 ± 0 ml (B3i), respectively, and were significantly different from each other (P <0.001). Slope in the pressure change over time showed a significant positive correlation with the collected saline solution (r = 0.91; P <0.001). Molecular and bacterial leakage was positive at the same implants, which also showed increased leakage values in the GEPT setup. The development of positive events in the timeline of the bacterial leakage evaluation corresponded well to the GEPT leakage model. Conclusion: The GEPT proved to be a reliable method to quantify leakage. The B3i showed the best sealing among the tested systems.",
keywords = "Abutment, Implants, In vitro, Leakage, Sealability",
author = "Anas Al-Jadaa and Thomas Attin and Timo Peltom{\"a}ki and Schmidlin, {Patrick R.}",
year = "2015",
month = "4",
day = "1",
doi = "10.1111/clr.12314",
language = "English",
volume = "26",
pages = "e1--e7",
journal = "Clinical Oral Implants Research",
issn = "0905-7161",
publisher = "Wiley",
number = "4",

}

RIS (suitable for import to EndNote) - Lataa

TY - JOUR

T1 - Comparison of three in vitro implant leakage testing methods

AU - Al-Jadaa, Anas

AU - Attin, Thomas

AU - Peltomäki, Timo

AU - Schmidlin, Patrick R.

PY - 2015/4/1

Y1 - 2015/4/1

N2 - Aims: To assess the accuracy and sensitivity in detecting implants leakage with a gas-enhanced permeation test (GEPT) and to compare with a molecular- and a bacterial-based leakage tests. Materials and methods: Three implants systems were tested (n = 20 per group): Nobel Biocare (NB), Astra Tech (AT) and Biomet 3i (B3i). Implants were mounted in PVC disks and were first tested for gas pressure change and infiltrated saline volume over 40 min. The same implants were then subjected to a molecular leakage evaluation using fluorescent Dextran for 28 days. After cleaning and sterilization, bacterial permeation (E. faecalis) was evaluated by selective media turbidity for another 28 days. Slopes in the pressure change and the perfused saline rate were used as a measure of leakage in the GEPT model and the times of positive events, that is, color change, after molecular and bacterial tests were recorded. Data were analyzed using Kolmogorov-Smirnov/Shapiro-Wilk, Kruskal-Wallis H and Spearman's Rho tests (P <0.05). Results: The gas and saline (ml) leakage values accounted for 0.85 ± 0.71 and 0.56 ± 0.50 ml (AT), 0.23 ± 0.030 and 0.12 ± 0.20 ml (NB) and 0.01 ± 0.01 and 0 ± 0 ml (B3i), respectively, and were significantly different from each other (P <0.001). Slope in the pressure change over time showed a significant positive correlation with the collected saline solution (r = 0.91; P <0.001). Molecular and bacterial leakage was positive at the same implants, which also showed increased leakage values in the GEPT setup. The development of positive events in the timeline of the bacterial leakage evaluation corresponded well to the GEPT leakage model. Conclusion: The GEPT proved to be a reliable method to quantify leakage. The B3i showed the best sealing among the tested systems.

AB - Aims: To assess the accuracy and sensitivity in detecting implants leakage with a gas-enhanced permeation test (GEPT) and to compare with a molecular- and a bacterial-based leakage tests. Materials and methods: Three implants systems were tested (n = 20 per group): Nobel Biocare (NB), Astra Tech (AT) and Biomet 3i (B3i). Implants were mounted in PVC disks and were first tested for gas pressure change and infiltrated saline volume over 40 min. The same implants were then subjected to a molecular leakage evaluation using fluorescent Dextran for 28 days. After cleaning and sterilization, bacterial permeation (E. faecalis) was evaluated by selective media turbidity for another 28 days. Slopes in the pressure change and the perfused saline rate were used as a measure of leakage in the GEPT model and the times of positive events, that is, color change, after molecular and bacterial tests were recorded. Data were analyzed using Kolmogorov-Smirnov/Shapiro-Wilk, Kruskal-Wallis H and Spearman's Rho tests (P <0.05). Results: The gas and saline (ml) leakage values accounted for 0.85 ± 0.71 and 0.56 ± 0.50 ml (AT), 0.23 ± 0.030 and 0.12 ± 0.20 ml (NB) and 0.01 ± 0.01 and 0 ± 0 ml (B3i), respectively, and were significantly different from each other (P <0.001). Slope in the pressure change over time showed a significant positive correlation with the collected saline solution (r = 0.91; P <0.001). Molecular and bacterial leakage was positive at the same implants, which also showed increased leakage values in the GEPT setup. The development of positive events in the timeline of the bacterial leakage evaluation corresponded well to the GEPT leakage model. Conclusion: The GEPT proved to be a reliable method to quantify leakage. The B3i showed the best sealing among the tested systems.

KW - Abutment

KW - Implants

KW - In vitro

KW - Leakage

KW - Sealability

UR - http://www.scopus.com/inward/record.url?scp=84924253667&partnerID=8YFLogxK

U2 - 10.1111/clr.12314

DO - 10.1111/clr.12314

M3 - Article

VL - 26

SP - e1-e7

JO - Clinical Oral Implants Research

JF - Clinical Oral Implants Research

SN - 0905-7161

IS - 4

ER -