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Composite Hydrogels Using Bioinspired Approach with in Situ Fast Gelation and Self-Healing Ability as Future Injectable Biomaterial

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Composite Hydrogels Using Bioinspired Approach with in Situ Fast Gelation and Self-Healing Ability as Future Injectable Biomaterial. / Khan, Musammir; Koivisto, Janne; Hukka, Terttu; Hokka, Mikko; Kellomäki, Minna.

julkaisussa: ACS Applied Materials & Interfaces, Vuosikerta 10, Nro 14, 11.04.2018, s. 11950-11960.

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Khan, Musammir ; Koivisto, Janne ; Hukka, Terttu ; Hokka, Mikko ; Kellomäki, Minna. / Composite Hydrogels Using Bioinspired Approach with in Situ Fast Gelation and Self-Healing Ability as Future Injectable Biomaterial. Julkaisussa: ACS Applied Materials & Interfaces. 2018 ; Vuosikerta 10, Nro 14. Sivut 11950-11960.

Bibtex - Lataa

@article{90c5d7424e4541e5b5865bf71a3f7990,
title = "Composite Hydrogels Using Bioinspired Approach with in Situ Fast Gelation and Self-Healing Ability as Future Injectable Biomaterial",
abstract = "Biopolymers are attractive candidates to fabricate biocompatible hydrogels, but the low water solubility of most of them at physiological pH has hindered their applications. To prepare a water-soluble derivative of chitosan (WSC) biopolymer, it was grafted with a small anionic amino acid, l-glutamic acid, using a single-step 1-ethyl-3-[3-dimethylaminopropyl]carbodiimide coupling reaction. This resulted in a zwitterion-tethered structure onto the polymer backbone. The degree of substitution range was 13–16 ± 1.25{\%}, which was controlled by varying the feeding reagent ratios. Differential scanning calorimetry- and X-ray diffraction-based analysis confirmed a transition from amorphous into a moderately amorphous/crystalline morphology after amino acid grafting, which made the derivative water-soluble at physiological pH. Composite hydrogels gelated within 60 s when using this WSC together with benzaldehyde-terminated 4-arm poly(ethylene glycol) as cross-linker. The compressive modulus of these hydrogels could be easily tuned between 4.0 ± 1.0 and 31 ± 2.5 kPa, either by changing the cross-linker concentration or total solid content in the final gel. The gels were injectable at the lowest cross-linker as well as total solid content, due to the enhanced elastic behavior. These hydrogels showed biodegradability during a 1 month incubation period in phosphate-buffered saline with weight remaining of 60 ± 1.5 and 44 ± 1.45{\%} at pHs 7.4 and 6.5, respectively. The cytocompatibility of the gels was tested using the fibroblast cell line (i.e., WI-38), which showed good cell viability on the gel surface. Therefore, these hydrogels could be an important injectable biomaterial for delivery purpose in the future.",
author = "Musammir Khan and Janne Koivisto and Terttu Hukka and Mikko Hokka and Minna Kellom{\"a}ki",
year = "2018",
month = "4",
day = "11",
doi = "10.1021/acsami.8b01351",
language = "English",
volume = "10",
pages = "11950--11960",
journal = "ACS Applied Materials & Interfaces",
issn = "1944-8244",
publisher = "American Chemical Society ACS",
number = "14",

}

RIS (suitable for import to EndNote) - Lataa

TY - JOUR

T1 - Composite Hydrogels Using Bioinspired Approach with in Situ Fast Gelation and Self-Healing Ability as Future Injectable Biomaterial

AU - Khan, Musammir

AU - Koivisto, Janne

AU - Hukka, Terttu

AU - Hokka, Mikko

AU - Kellomäki, Minna

PY - 2018/4/11

Y1 - 2018/4/11

N2 - Biopolymers are attractive candidates to fabricate biocompatible hydrogels, but the low water solubility of most of them at physiological pH has hindered their applications. To prepare a water-soluble derivative of chitosan (WSC) biopolymer, it was grafted with a small anionic amino acid, l-glutamic acid, using a single-step 1-ethyl-3-[3-dimethylaminopropyl]carbodiimide coupling reaction. This resulted in a zwitterion-tethered structure onto the polymer backbone. The degree of substitution range was 13–16 ± 1.25%, which was controlled by varying the feeding reagent ratios. Differential scanning calorimetry- and X-ray diffraction-based analysis confirmed a transition from amorphous into a moderately amorphous/crystalline morphology after amino acid grafting, which made the derivative water-soluble at physiological pH. Composite hydrogels gelated within 60 s when using this WSC together with benzaldehyde-terminated 4-arm poly(ethylene glycol) as cross-linker. The compressive modulus of these hydrogels could be easily tuned between 4.0 ± 1.0 and 31 ± 2.5 kPa, either by changing the cross-linker concentration or total solid content in the final gel. The gels were injectable at the lowest cross-linker as well as total solid content, due to the enhanced elastic behavior. These hydrogels showed biodegradability during a 1 month incubation period in phosphate-buffered saline with weight remaining of 60 ± 1.5 and 44 ± 1.45% at pHs 7.4 and 6.5, respectively. The cytocompatibility of the gels was tested using the fibroblast cell line (i.e., WI-38), which showed good cell viability on the gel surface. Therefore, these hydrogels could be an important injectable biomaterial for delivery purpose in the future.

AB - Biopolymers are attractive candidates to fabricate biocompatible hydrogels, but the low water solubility of most of them at physiological pH has hindered their applications. To prepare a water-soluble derivative of chitosan (WSC) biopolymer, it was grafted with a small anionic amino acid, l-glutamic acid, using a single-step 1-ethyl-3-[3-dimethylaminopropyl]carbodiimide coupling reaction. This resulted in a zwitterion-tethered structure onto the polymer backbone. The degree of substitution range was 13–16 ± 1.25%, which was controlled by varying the feeding reagent ratios. Differential scanning calorimetry- and X-ray diffraction-based analysis confirmed a transition from amorphous into a moderately amorphous/crystalline morphology after amino acid grafting, which made the derivative water-soluble at physiological pH. Composite hydrogels gelated within 60 s when using this WSC together with benzaldehyde-terminated 4-arm poly(ethylene glycol) as cross-linker. The compressive modulus of these hydrogels could be easily tuned between 4.0 ± 1.0 and 31 ± 2.5 kPa, either by changing the cross-linker concentration or total solid content in the final gel. The gels were injectable at the lowest cross-linker as well as total solid content, due to the enhanced elastic behavior. These hydrogels showed biodegradability during a 1 month incubation period in phosphate-buffered saline with weight remaining of 60 ± 1.5 and 44 ± 1.45% at pHs 7.4 and 6.5, respectively. The cytocompatibility of the gels was tested using the fibroblast cell line (i.e., WI-38), which showed good cell viability on the gel surface. Therefore, these hydrogels could be an important injectable biomaterial for delivery purpose in the future.

U2 - 10.1021/acsami.8b01351

DO - 10.1021/acsami.8b01351

M3 - Article

VL - 10

SP - 11950

EP - 11960

JO - ACS Applied Materials & Interfaces

JF - ACS Applied Materials & Interfaces

SN - 1944-8244

IS - 14

ER -