TUTCRIS - Tampereen teknillinen yliopisto

TUTCRIS

Differential mobility spectrometry classification of bacteria

Tutkimustuotosvertaisarvioitu

Yksityiskohdat

AlkuperäiskieliEnglanti
Sivut233-240
Sivumäärä8
JulkaisuFUTURE MICROBIOLOGY
Vuosikerta15
Numero4
DOI - pysyväislinkit
TilaJulkaistu - 1 maaliskuuta 2020
OKM-julkaisutyyppiA1 Alkuperäisartikkeli

Tiivistelmä

Aim: Rapid identification of bacteria would facilitate timely initiation of therapy and improve cost-effectiveness of treatment. Traditional methods (culture, PCR) require reagents, consumables and hours to days to complete the identification. In this study, we examined whether differential mobility spectrometry could classify most common bacterial species, genera and between Gram status within minutes. Materials & methods: Cultured bacterial sample gaseous headspaces were measured with differential mobility spectrometry and data analyzed using k-nearest-neighbor and leave-one-out cross-validation. Results: Differential mobility spectrometry achieved a correct classification rate 70.7% for all bacterial species. For bacterial genera, the rate was 77.6% and between Gram status, 89.1%. Conclusion: Largest difficulties arose in distinguishing bacteria of the same genus. Future improvement of the sensor characteristics may improve the classification accuracy.

!!ASJC Scopus subject areas

Tutkimusalat

Julkaisufoorumi-taso

Tilastokeskuksen tieteenalat