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Direct Laser Writing of Tubular Microtowers for 3D Culture of Human Pluripotent Stem Cell-Derived Neuronal Cells

Tutkimustuotosvertaisarvioitu

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Direct Laser Writing of Tubular Microtowers for 3D Culture of Human Pluripotent Stem Cell-Derived Neuronal Cells. / Turunen, Sanna; Joki, Tiina; Hiltunen, Maiju L; Ihalainen, Teemu O; Narkilahti, Susanna; Kellomäki, Minna.

julkaisussa: ACS Applied Materials and Interfaces, Vuosikerta 9, Nro 31, 09.08.2017, s. 25717-25730.

Tutkimustuotosvertaisarvioitu

Harvard

Turunen, S, Joki, T, Hiltunen, ML, Ihalainen, TO, Narkilahti, S & Kellomäki, M 2017, 'Direct Laser Writing of Tubular Microtowers for 3D Culture of Human Pluripotent Stem Cell-Derived Neuronal Cells', ACS Applied Materials and Interfaces, Vuosikerta. 9, Nro 31, Sivut 25717-25730. https://doi.org/10.1021/acsami.7b05536

APA

Vancouver

Author

Turunen, Sanna ; Joki, Tiina ; Hiltunen, Maiju L ; Ihalainen, Teemu O ; Narkilahti, Susanna ; Kellomäki, Minna. / Direct Laser Writing of Tubular Microtowers for 3D Culture of Human Pluripotent Stem Cell-Derived Neuronal Cells. Julkaisussa: ACS Applied Materials and Interfaces. 2017 ; Vuosikerta 9, Nro 31. Sivut 25717-25730.

Bibtex - Lataa

@article{53347928c599477ab9de369ec4d0fcc1,
title = "Direct Laser Writing of Tubular Microtowers for 3D Culture of Human Pluripotent Stem Cell-Derived Neuronal Cells",
abstract = "As the complex structure of nervous tissue cannot be mimicked in two-dimensional (2D) cultures, the development of three-dimensional (3D) neuronal cell culture platforms is a topical issue in the field of neuroscience and neural tissue engineering. Computer-assisted laser-based fabrication techniques such as direct laser writing by two-photon polymerization (2PP-DLW) offer a versatile tool to fabricate 3D cell culture platforms with highly ordered geometries in the size scale of natural 3D cell environments. In this study, we present the design and 2PP-DLW fabrication process of a novel 3D neuronal cell culture platform based on tubular microtowers. The platform facilitates efficient long-term 3D culturing of human neuronal cells and supports neurite orientation and 3D network formation. Microtower designs both with or without intraluminal guidance cues and/or openings in the tower wall are designed and successfully fabricated from Ormocomp. Three of the microtower designs are chosen for the final culture platform: a design with openings in the wall and intralumial guidance cues (webs and pillars), a design with openings but without intraluminal structures, and a plain cylinder design. The proposed culture platform offers a promising concept for future 3D cultures in the field of neuroscience.",
keywords = "Journal Article",
author = "Sanna Turunen and Tiina Joki and Hiltunen, {Maiju L} and Ihalainen, {Teemu O} and Susanna Narkilahti and Minna Kellom{\"a}ki",
year = "2017",
month = "8",
day = "9",
doi = "10.1021/acsami.7b05536",
language = "English",
volume = "9",
pages = "25717--25730",
journal = "ACS Applied Materials & Interfaces",
issn = "1944-8244",
publisher = "American Chemical Society ACS",
number = "31",

}

RIS (suitable for import to EndNote) - Lataa

TY - JOUR

T1 - Direct Laser Writing of Tubular Microtowers for 3D Culture of Human Pluripotent Stem Cell-Derived Neuronal Cells

AU - Turunen, Sanna

AU - Joki, Tiina

AU - Hiltunen, Maiju L

AU - Ihalainen, Teemu O

AU - Narkilahti, Susanna

AU - Kellomäki, Minna

PY - 2017/8/9

Y1 - 2017/8/9

N2 - As the complex structure of nervous tissue cannot be mimicked in two-dimensional (2D) cultures, the development of three-dimensional (3D) neuronal cell culture platforms is a topical issue in the field of neuroscience and neural tissue engineering. Computer-assisted laser-based fabrication techniques such as direct laser writing by two-photon polymerization (2PP-DLW) offer a versatile tool to fabricate 3D cell culture platforms with highly ordered geometries in the size scale of natural 3D cell environments. In this study, we present the design and 2PP-DLW fabrication process of a novel 3D neuronal cell culture platform based on tubular microtowers. The platform facilitates efficient long-term 3D culturing of human neuronal cells and supports neurite orientation and 3D network formation. Microtower designs both with or without intraluminal guidance cues and/or openings in the tower wall are designed and successfully fabricated from Ormocomp. Three of the microtower designs are chosen for the final culture platform: a design with openings in the wall and intralumial guidance cues (webs and pillars), a design with openings but without intraluminal structures, and a plain cylinder design. The proposed culture platform offers a promising concept for future 3D cultures in the field of neuroscience.

AB - As the complex structure of nervous tissue cannot be mimicked in two-dimensional (2D) cultures, the development of three-dimensional (3D) neuronal cell culture platforms is a topical issue in the field of neuroscience and neural tissue engineering. Computer-assisted laser-based fabrication techniques such as direct laser writing by two-photon polymerization (2PP-DLW) offer a versatile tool to fabricate 3D cell culture platforms with highly ordered geometries in the size scale of natural 3D cell environments. In this study, we present the design and 2PP-DLW fabrication process of a novel 3D neuronal cell culture platform based on tubular microtowers. The platform facilitates efficient long-term 3D culturing of human neuronal cells and supports neurite orientation and 3D network formation. Microtower designs both with or without intraluminal guidance cues and/or openings in the tower wall are designed and successfully fabricated from Ormocomp. Three of the microtower designs are chosen for the final culture platform: a design with openings in the wall and intralumial guidance cues (webs and pillars), a design with openings but without intraluminal structures, and a plain cylinder design. The proposed culture platform offers a promising concept for future 3D cultures in the field of neuroscience.

KW - Journal Article

U2 - 10.1021/acsami.7b05536

DO - 10.1021/acsami.7b05536

M3 - Article

VL - 9

SP - 25717

EP - 25730

JO - ACS Applied Materials & Interfaces

JF - ACS Applied Materials & Interfaces

SN - 1944-8244

IS - 31

ER -