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Effects of single and fractionated irradiation on natural killer cell populations: Radiobiological characteristics of viability and cytotoxicity in vitro

Tutkimustuotosvertaisarvioitu

Standard

Effects of single and fractionated irradiation on natural killer cell populations : Radiobiological characteristics of viability and cytotoxicity in vitro. / Hietanen, Tenho; Pitkänen, Maunu; Kapanen, Mika; Kellokumpu-Lehtinen, Pirkko Liisa.

julkaisussa: Anticancer Research, Vuosikerta 35, Nro 10, 01.10.2015, s. 5193-5200.

Tutkimustuotosvertaisarvioitu

Harvard

Hietanen, T, Pitkänen, M, Kapanen, M & Kellokumpu-Lehtinen, PL 2015, 'Effects of single and fractionated irradiation on natural killer cell populations: Radiobiological characteristics of viability and cytotoxicity in vitro', Anticancer Research, Vuosikerta. 35, Nro 10, Sivut 5193-5200.

APA

Hietanen, T., Pitkänen, M., Kapanen, M., & Kellokumpu-Lehtinen, P. L. (2015). Effects of single and fractionated irradiation on natural killer cell populations: Radiobiological characteristics of viability and cytotoxicity in vitro. Anticancer Research, 35(10), 5193-5200.

Vancouver

Hietanen T, Pitkänen M, Kapanen M, Kellokumpu-Lehtinen PL. Effects of single and fractionated irradiation on natural killer cell populations: Radiobiological characteristics of viability and cytotoxicity in vitro. Anticancer Research. 2015 loka 1;35(10):5193-5200.

Author

Hietanen, Tenho ; Pitkänen, Maunu ; Kapanen, Mika ; Kellokumpu-Lehtinen, Pirkko Liisa. / Effects of single and fractionated irradiation on natural killer cell populations : Radiobiological characteristics of viability and cytotoxicity in vitro. Julkaisussa: Anticancer Research. 2015 ; Vuosikerta 35, Nro 10. Sivut 5193-5200.

Bibtex - Lataa

@article{cb01c0ec528a44db890fbceb7b464ce2,
title = "Effects of single and fractionated irradiation on natural killer cell populations: Radiobiological characteristics of viability and cytotoxicity in vitro",
abstract = "Background: Natural killer (NK) cells are important in destroying tumor cells. However, they are damaged by radiation therapy. We studied the effects of single and fractionated irradiation on the viability and cytotoxicity of human non-selected NK cells and sub-groups with cluster of differentiation (CD) CD16+ and CD56+ in vitro. Only very few studies dealing with the standard radiobiological parameters for characterizing NK cells exist in the literature. Materials and Methods: NK cell populations were isolated from buffy coats using different methods and irradiated with single doses up to 80 Gy and fractionated doses of 10 or 30 Gy with different numbers of applications and at different intervals. The study end-points were viability using propidium iodide (PI), trypan blue and intracellular adenosine triphosphate (ATP) assays, and cytotoxicity using the 51Cr-release assay. The standard radiobiological parameters α and β of the linear-quadratic (L-Q) model and the mean inactivation dose D taken as the area under the curve (AUC) were calculated to characterize the radiosensitivity of different NK cell populations. Results: The AUC values of the 51Cr release data in the dose range of 0-40 Gy were as follows: for non-selected NK cells, 23.6- 20.9 Gy; for CD16+ and CD56+ cells, 14.5-13.2 Gy. The AUC values of ATP, trypan blue and propidium iodide methods equally well described the viability of irradiated NK cells. The α/β ratio for cytotoxicity and viability data in the L-Q model corresponded to the acutely responding tissues. Splitting a 30-Gy dose into two fractions applied at different intervals caused a significant rise in ATP levels and cytotoxicity. Dividing the total dose into four doses applied at fixed intervals also resulted in significant elevations of ATP content and cytotoxicity of NK cells at 10 Gy. Conclusion: According to the L-Q method, irradiated NK cells behaved similarly to acutely responding human tissues with respect to cytotoxicity and viability. The AUC proved very useful for comparing the effects of irradiation on NK cells.",
keywords = "Cytotoxicity, Enrichment methods, Irradiation, Natural killer cell subsets, Natural killer cells, Radiobiological models, Viability",
author = "Tenho Hietanen and Maunu Pitk{\"a}nen and Mika Kapanen and Kellokumpu-Lehtinen, {Pirkko Liisa}",
year = "2015",
month = "10",
day = "1",
language = "English",
volume = "35",
pages = "5193--5200",
journal = "Anticancer Research",
issn = "0250-7005",
publisher = "International Institute of Anticancer Research",
number = "10",

}

RIS (suitable for import to EndNote) - Lataa

TY - JOUR

T1 - Effects of single and fractionated irradiation on natural killer cell populations

T2 - Radiobiological characteristics of viability and cytotoxicity in vitro

AU - Hietanen, Tenho

AU - Pitkänen, Maunu

AU - Kapanen, Mika

AU - Kellokumpu-Lehtinen, Pirkko Liisa

PY - 2015/10/1

Y1 - 2015/10/1

N2 - Background: Natural killer (NK) cells are important in destroying tumor cells. However, they are damaged by radiation therapy. We studied the effects of single and fractionated irradiation on the viability and cytotoxicity of human non-selected NK cells and sub-groups with cluster of differentiation (CD) CD16+ and CD56+ in vitro. Only very few studies dealing with the standard radiobiological parameters for characterizing NK cells exist in the literature. Materials and Methods: NK cell populations were isolated from buffy coats using different methods and irradiated with single doses up to 80 Gy and fractionated doses of 10 or 30 Gy with different numbers of applications and at different intervals. The study end-points were viability using propidium iodide (PI), trypan blue and intracellular adenosine triphosphate (ATP) assays, and cytotoxicity using the 51Cr-release assay. The standard radiobiological parameters α and β of the linear-quadratic (L-Q) model and the mean inactivation dose D taken as the area under the curve (AUC) were calculated to characterize the radiosensitivity of different NK cell populations. Results: The AUC values of the 51Cr release data in the dose range of 0-40 Gy were as follows: for non-selected NK cells, 23.6- 20.9 Gy; for CD16+ and CD56+ cells, 14.5-13.2 Gy. The AUC values of ATP, trypan blue and propidium iodide methods equally well described the viability of irradiated NK cells. The α/β ratio for cytotoxicity and viability data in the L-Q model corresponded to the acutely responding tissues. Splitting a 30-Gy dose into two fractions applied at different intervals caused a significant rise in ATP levels and cytotoxicity. Dividing the total dose into four doses applied at fixed intervals also resulted in significant elevations of ATP content and cytotoxicity of NK cells at 10 Gy. Conclusion: According to the L-Q method, irradiated NK cells behaved similarly to acutely responding human tissues with respect to cytotoxicity and viability. The AUC proved very useful for comparing the effects of irradiation on NK cells.

AB - Background: Natural killer (NK) cells are important in destroying tumor cells. However, they are damaged by radiation therapy. We studied the effects of single and fractionated irradiation on the viability and cytotoxicity of human non-selected NK cells and sub-groups with cluster of differentiation (CD) CD16+ and CD56+ in vitro. Only very few studies dealing with the standard radiobiological parameters for characterizing NK cells exist in the literature. Materials and Methods: NK cell populations were isolated from buffy coats using different methods and irradiated with single doses up to 80 Gy and fractionated doses of 10 or 30 Gy with different numbers of applications and at different intervals. The study end-points were viability using propidium iodide (PI), trypan blue and intracellular adenosine triphosphate (ATP) assays, and cytotoxicity using the 51Cr-release assay. The standard radiobiological parameters α and β of the linear-quadratic (L-Q) model and the mean inactivation dose D taken as the area under the curve (AUC) were calculated to characterize the radiosensitivity of different NK cell populations. Results: The AUC values of the 51Cr release data in the dose range of 0-40 Gy were as follows: for non-selected NK cells, 23.6- 20.9 Gy; for CD16+ and CD56+ cells, 14.5-13.2 Gy. The AUC values of ATP, trypan blue and propidium iodide methods equally well described the viability of irradiated NK cells. The α/β ratio for cytotoxicity and viability data in the L-Q model corresponded to the acutely responding tissues. Splitting a 30-Gy dose into two fractions applied at different intervals caused a significant rise in ATP levels and cytotoxicity. Dividing the total dose into four doses applied at fixed intervals also resulted in significant elevations of ATP content and cytotoxicity of NK cells at 10 Gy. Conclusion: According to the L-Q method, irradiated NK cells behaved similarly to acutely responding human tissues with respect to cytotoxicity and viability. The AUC proved very useful for comparing the effects of irradiation on NK cells.

KW - Cytotoxicity

KW - Enrichment methods

KW - Irradiation

KW - Natural killer cell subsets

KW - Natural killer cells

KW - Radiobiological models

KW - Viability

UR - http://www.scopus.com/inward/record.url?scp=84942743898&partnerID=8YFLogxK

M3 - Article

VL - 35

SP - 5193

EP - 5200

JO - Anticancer Research

JF - Anticancer Research

SN - 0250-7005

IS - 10

ER -