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Efficient preparation of shuffled DNA libraries through recombination (Gateway) cloning

Tutkimustuotosvertaisarvioitu

Standard

Efficient preparation of shuffled DNA libraries through recombination (Gateway) cloning. / Lehtonen, Soili I.; Taskinen, Barbara; Ojala, Elina; Kukkurainen, Sampo; Rahikainen, Rolle; Riihimäki, Tiina A.; Laitinen, Olli H.; Kulomaa, Markku S.; Hytönen, Vesa P.

julkaisussa: Protein Engineering Design and Selection, Vuosikerta 28, Nro 1, 2015, s. 23-28.

Tutkimustuotosvertaisarvioitu

Harvard

Lehtonen, SI, Taskinen, B, Ojala, E, Kukkurainen, S, Rahikainen, R, Riihimäki, TA, Laitinen, OH, Kulomaa, MS & Hytönen, VP 2015, 'Efficient preparation of shuffled DNA libraries through recombination (Gateway) cloning', Protein Engineering Design and Selection, Vuosikerta. 28, Nro 1, Sivut 23-28. https://doi.org/10.1093/protein/gzu050

APA

Lehtonen, S. I., Taskinen, B., Ojala, E., Kukkurainen, S., Rahikainen, R., Riihimäki, T. A., ... Hytönen, V. P. (2015). Efficient preparation of shuffled DNA libraries through recombination (Gateway) cloning. Protein Engineering Design and Selection, 28(1), 23-28. https://doi.org/10.1093/protein/gzu050

Vancouver

Lehtonen SI, Taskinen B, Ojala E, Kukkurainen S, Rahikainen R, Riihimäki TA et al. Efficient preparation of shuffled DNA libraries through recombination (Gateway) cloning. Protein Engineering Design and Selection. 2015;28(1):23-28. https://doi.org/10.1093/protein/gzu050

Author

Lehtonen, Soili I. ; Taskinen, Barbara ; Ojala, Elina ; Kukkurainen, Sampo ; Rahikainen, Rolle ; Riihimäki, Tiina A. ; Laitinen, Olli H. ; Kulomaa, Markku S. ; Hytönen, Vesa P. / Efficient preparation of shuffled DNA libraries through recombination (Gateway) cloning. Julkaisussa: Protein Engineering Design and Selection. 2015 ; Vuosikerta 28, Nro 1. Sivut 23-28.

Bibtex - Lataa

@article{991913a6d099449890dc83d292303d1d,
title = "Efficient preparation of shuffled DNA libraries through recombination (Gateway) cloning",
abstract = "Efficient and robust subcloning is essential for the construction of high-diversity DNA libraries in the field of directed evolution. We have developed a more efficient method for the subcloning of DNAshuffled libraries by employing recombination cloning (Gateway). The Gateway cloning procedure was performed directly after the gene reassembly reaction, without additional purification and amplification steps, thus simplifying the conventional DNA shuffling protocols. Recombination-based cloning, directly from the heterologous reassembly reaction, conserved the high quality of the library and reduced the time required for the library construction. The described method is generally compatible for the construction of DNA-shuffled gene libraries.",
keywords = "Directed evolution, DNA library, DNA shuffling, Phage display recombination cloning",
author = "Lehtonen, {Soili I.} and Barbara Taskinen and Elina Ojala and Sampo Kukkurainen and Rolle Rahikainen and Riihim{\"a}ki, {Tiina A.} and Laitinen, {Olli H.} and Kulomaa, {Markku S.} and Hyt{\"o}nen, {Vesa P.}",
year = "2015",
doi = "10.1093/protein/gzu050",
language = "English",
volume = "28",
pages = "23--28",
journal = "Protein Engineering Design and Selection",
issn = "1741-0126",
publisher = "Oxford University Press",
number = "1",

}

RIS (suitable for import to EndNote) - Lataa

TY - JOUR

T1 - Efficient preparation of shuffled DNA libraries through recombination (Gateway) cloning

AU - Lehtonen, Soili I.

AU - Taskinen, Barbara

AU - Ojala, Elina

AU - Kukkurainen, Sampo

AU - Rahikainen, Rolle

AU - Riihimäki, Tiina A.

AU - Laitinen, Olli H.

AU - Kulomaa, Markku S.

AU - Hytönen, Vesa P.

PY - 2015

Y1 - 2015

N2 - Efficient and robust subcloning is essential for the construction of high-diversity DNA libraries in the field of directed evolution. We have developed a more efficient method for the subcloning of DNAshuffled libraries by employing recombination cloning (Gateway). The Gateway cloning procedure was performed directly after the gene reassembly reaction, without additional purification and amplification steps, thus simplifying the conventional DNA shuffling protocols. Recombination-based cloning, directly from the heterologous reassembly reaction, conserved the high quality of the library and reduced the time required for the library construction. The described method is generally compatible for the construction of DNA-shuffled gene libraries.

AB - Efficient and robust subcloning is essential for the construction of high-diversity DNA libraries in the field of directed evolution. We have developed a more efficient method for the subcloning of DNAshuffled libraries by employing recombination cloning (Gateway). The Gateway cloning procedure was performed directly after the gene reassembly reaction, without additional purification and amplification steps, thus simplifying the conventional DNA shuffling protocols. Recombination-based cloning, directly from the heterologous reassembly reaction, conserved the high quality of the library and reduced the time required for the library construction. The described method is generally compatible for the construction of DNA-shuffled gene libraries.

KW - Directed evolution

KW - DNA library

KW - DNA shuffling

KW - Phage display recombination cloning

UR - http://www.scopus.com/inward/record.url?scp=84983121996&partnerID=8YFLogxK

U2 - 10.1093/protein/gzu050

DO - 10.1093/protein/gzu050

M3 - Article

VL - 28

SP - 23

EP - 28

JO - Protein Engineering Design and Selection

JF - Protein Engineering Design and Selection

SN - 1741-0126

IS - 1

ER -